ENSG00000145414

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000274054 ENSG00000145414 HEK293_OSMI2_2hA HEK293_TMG_2hB NAF1 protein_coding protein_coding 21.85532 18.87638 26.96543 0.8479586 0.9461227 0.5142999 12.3106716 12.52940488 13.263898 0.75793834 0.9823650 0.08212325 0.56480000 0.66363333 0.49073333 -0.17290000 0.001140996 0.001140996 FALSE TRUE
ENST00000422287 ENSG00000145414 HEK293_OSMI2_2hA HEK293_TMG_2hB NAF1 protein_coding protein_coding 21.85532 18.87638 26.96543 0.8479586 0.9461227 0.5142999 2.7734811 1.33502711 4.306741 0.41418699 0.4812868 1.68230735 0.11907083 0.07006667 0.15960000 0.08953333 0.042944351 0.001140996 FALSE TRUE
ENST00000502973 ENSG00000145414 HEK293_OSMI2_2hA HEK293_TMG_2hB NAF1 protein_coding retained_intron 21.85532 18.87638 26.96543 0.8479586 0.9461227 0.5142999 1.6050090 1.63923519 2.673144 0.11779360 0.3885892 0.70212731 0.07457917 0.08740000 0.09836667 0.01096667 0.805390449 0.001140996 TRUE TRUE
MSTRG.25674.2 ENSG00000145414 HEK293_OSMI2_2hA HEK293_TMG_2hB NAF1 protein_coding   21.85532 18.87638 26.96543 0.8479586 0.9461227 0.5142999 1.2920492 1.27264532 1.209955 0.26274186 0.1254274 -0.07229419 0.06508750 0.06640000 0.04476667 -0.02163333 0.340946514 0.001140996 TRUE TRUE
MSTRG.25674.7 ENSG00000145414 HEK293_OSMI2_2hA HEK293_TMG_2hB NAF1 protein_coding   21.85532 18.87638 26.96543 0.8479586 0.9461227 0.5142999 0.4694877 0.09275163 1.606929 0.09275163 1.3658359 3.97602269 0.02170833 0.00540000 0.06233333 0.05693333 0.607932330 0.001140996 FALSE TRUE
MSTRG.25674.9 ENSG00000145414 HEK293_OSMI2_2hA HEK293_TMG_2hB NAF1 protein_coding   21.85532 18.87638 26.96543 0.8479586 0.9461227 0.5142999 1.3979574 1.39975688 1.201225 0.12464994 0.1731054 -0.21897945 0.06790000 0.07470000 0.04466667 -0.03003333 0.168030560 0.001140996 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000145414 E001 0.147249 0.0440836968 5.541393e-01   4 163084107 163084163 57 - 0.117 0.000 -9.318
ENSG00000145414 E002 9.982561 0.0017839745 3.822776e-04 1.706200e-03 4 163104062 163104150 89 - 0.818 1.186 1.364
ENSG00000145414 E003 15.354219 0.0011580923 3.246131e-03 1.099063e-02 4 163104151 163104316 166 - 1.073 1.319 0.871
ENSG00000145414 E004 3.306227 0.1340394885 6.140012e-02 1.289887e-01 4 163110073 163110226 154 - 0.798 0.378 -1.932
ENSG00000145414 E005 2.880812 0.0056002239 3.273692e-02 7.727372e-02 4 163110227 163110290 64 - 0.727 0.375 -1.663
ENSG00000145414 E006 2.245705 0.1643864951 6.601451e-01 7.688445e-01 4 163119612 163119671 60 - 0.541 0.444 -0.475
ENSG00000145414 E007 45.444878 0.0117101988 5.814289e-05 3.223210e-04 4 163126708 163126881 174 - 1.804 1.491 -1.062
ENSG00000145414 E008 46.772345 0.0005028785 1.705800e-10 2.893095e-09 4 163126882 163127061 180 - 1.828 1.483 -1.173
ENSG00000145414 E009 32.071376 0.0006337786 2.465985e-11 4.820196e-10 4 163127062 163127115 54 - 1.696 1.258 -1.508
ENSG00000145414 E010 66.210629 0.0004086434 3.679262e-11 6.976531e-10 4 163128669 163128851 183 - 1.665 1.945 0.946
ENSG00000145414 E011 59.013257 0.0023237075 4.852739e-04 2.103842e-03 4 163128852 163128896 45 - 1.687 1.853 0.559
ENSG00000145414 E012 89.824832 0.0003712201 6.958707e-06 4.795585e-05 4 163128897 163129101 205 - 1.873 2.030 0.528
ENSG00000145414 E013 45.065527 0.0005468273 5.463501e-03 1.719113e-02 4 163129102 163129119 18 - 1.592 1.727 0.457
ENSG00000145414 E014 83.057438 0.0016591393 1.962751e-03 7.109563e-03 4 163129120 163129178 59 - 1.862 1.983 0.404
ENSG00000145414 E015 197.553851 0.0003790647 6.494603e-04 2.714041e-03 4 163129179 163129348 170 - 2.260 2.336 0.252
ENSG00000145414 E016 184.986041 0.0002244177 2.096485e-02 5.351651e-02 4 163133154 163133256 103 - 2.248 2.296 0.162
ENSG00000145414 E017 3.555761 0.0043965770 6.175740e-04 2.597925e-03 4 163133257 163133642 386 - 0.857 0.307 -2.594
ENSG00000145414 E018 8.392793 0.0019517317 1.029969e-05 6.828028e-05 4 163135026 163136320 1295 - 1.174 0.645 -2.027
ENSG00000145414 E019 157.495063 0.0005822962 4.293739e-01 5.721024e-01 4 163137199 163137250 52 - 2.199 2.211 0.042
ENSG00000145414 E020 182.024241 0.0002396333 8.807529e-01 9.275343e-01 4 163140223 163140383 161 - 2.274 2.260 -0.047
ENSG00000145414 E021 132.739612 0.0006716781 6.211506e-01 7.381638e-01 4 163145782 163145864 83 - 2.142 2.115 -0.087
ENSG00000145414 E022 169.372202 0.0014231633 1.682002e-02 4.453585e-02 4 163148341 163148434 94 - 2.276 2.191 -0.284
ENSG00000145414 E023 39.772292 0.0076951860 8.739217e-01 9.229672e-01 4 163156964 163157454 491 - 1.612 1.614 0.007
ENSG00000145414 E024 21.574711 0.0008561397 2.389787e-01 3.729451e-01 4 163158225 163158327 103 - 1.401 1.304 -0.335
ENSG00000145414 E025 209.720293 0.0004145708 2.371746e-02 5.922939e-02 4 163164217 163164391 175 - 2.358 2.291 -0.223
ENSG00000145414 E026 304.568480 0.0065285046 1.287209e-01 2.323740e-01 4 163166363 163166978 616 - 2.523 2.446 -0.258