ENSG00000145388

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000388822 ENSG00000145388 HEK293_OSMI2_2hA HEK293_TMG_2hB METTL14 protein_coding protein_coding 10.8969 2.46505 18.28433 0.1040058 0.60908 2.885867 2.3552292 0.6721683 4.3516137 0.09953307 0.28309032 2.676662 0.25852083 0.27586667 0.2378000 -0.03806667 7.554619e-01 2.071276e-08 FALSE TRUE
MSTRG.25363.1 ENSG00000145388 HEK293_OSMI2_2hA HEK293_TMG_2hB METTL14 protein_coding   10.8969 2.46505 18.28433 0.1040058 0.60908 2.885867 0.5299718 0.3145180 0.2732244 0.01682770 0.05260279 -0.196353 0.05460833 0.12813333 0.0151000 -0.11303333 2.071276e-08 2.071276e-08 FALSE TRUE
MSTRG.25363.2 ENSG00000145388 HEK293_OSMI2_2hA HEK293_TMG_2hB METTL14 protein_coding   10.8969 2.46505 18.28433 0.1040058 0.60908 2.885867 5.5784223 1.3195648 9.2478200 0.08078248 0.45846675 2.799718 0.50206250 0.53653333 0.5052667 -0.03126667 8.023727e-01 2.071276e-08 FALSE TRUE
MSTRG.25363.5 ENSG00000145388 HEK293_OSMI2_2hA HEK293_TMG_2hB METTL14 protein_coding   10.8969 2.46505 18.28433 0.1040058 0.60908 2.885867 1.6663202 0.1587989 4.1999469 0.15879886 0.38166321 4.640425 0.13548333 0.05946667 0.2302667 0.17080000 1.454422e-01 2.071276e-08 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000145388 E001 0.5891098 0.0183214599 6.030109e-01 7.238767e-01 4 118685384 118685387 4 + 0.194 0.001 -8.620
ENSG00000145388 E002 1.8791015 0.0085485406 4.140865e-01 5.574919e-01 4 118685388 118685391 4 + 0.356 0.512 0.825
ENSG00000145388 E003 1.8791015 0.0085485406 4.140865e-01 5.574919e-01 4 118685392 118685393 2 + 0.356 0.512 0.825
ENSG00000145388 E004 3.8869044 0.0044388632 9.725530e-01 9.868732e-01 4 118685394 118685399 6 + 0.598 0.602 0.017
ENSG00000145388 E005 9.8608962 0.0023077830 5.299529e-02 1.144776e-01 4 118685400 118685412 13 + 0.977 0.677 -1.176
ENSG00000145388 E006 12.9652339 0.0013705490 1.391279e-02 3.802877e-02 4 118685413 118685414 2 + 1.090 0.740 -1.328
ENSG00000145388 E007 59.9886902 0.0003911728 2.546139e-02 6.282753e-02 4 118685415 118685600 186 + 1.701 1.559 -0.483
ENSG00000145388 E008 0.0000000       4 118686550 118686699 150 +      
ENSG00000145388 E009 59.9208822 0.0003943864 4.522455e-01 5.931184e-01 4 118687923 118688011 89 + 1.688 1.641 -0.159
ENSG00000145388 E010 44.7966016 0.0016553360 3.738499e-01 5.182703e-01 4 118689370 118689404 35 + 1.567 1.501 -0.223
ENSG00000145388 E011 53.0207101 0.0017466969 4.717439e-02 1.041420e-01 4 118689405 118689457 53 + 1.650 1.511 -0.472
ENSG00000145388 E012 0.0000000       4 118689458 118689468 11 +      
ENSG00000145388 E013 32.8807360 0.0007307785 1.901497e-03 6.919441e-03 4 118691532 118691533 2 + 1.466 1.183 -0.987
ENSG00000145388 E014 34.8371568 0.0006622114 2.567251e-03 8.964629e-03 4 118691534 118691541 8 + 1.488 1.224 -0.920
ENSG00000145388 E015 54.1653891 0.0012386432 8.905460e-02 1.736842e-01 4 118691542 118691612 71 + 1.655 1.541 -0.390
ENSG00000145388 E016 62.1043994 0.0055997680 2.043481e-01 3.315985e-01 4 118691981 118692068 88 + 1.708 1.618 -0.305
ENSG00000145388 E017 62.4867427 0.0007508299 3.722730e-01 5.167232e-01 4 118694436 118694526 91 + 1.704 1.648 -0.188
ENSG00000145388 E018 53.2459577 0.0004956688 2.256689e-01 3.571770e-01 4 118697182 118697210 29 + 1.640 1.559 -0.274
ENSG00000145388 E019 74.1157760 0.0107081000 5.887524e-01 7.119296e-01 4 118697211 118697323 113 + 1.776 1.730 -0.156
ENSG00000145388 E020 0.8232405 0.1329228490 7.766013e-01 8.561244e-01 4 118697324 118697355 32 + 0.195 0.245 0.419
ENSG00000145388 E021 66.5095715 0.0070961063 1.669895e-01 2.842709e-01 4 118700550 118700642 93 + 1.738 1.635 -0.352
ENSG00000145388 E022 82.1672738 0.0071225878 6.498042e-01 7.609406e-01 4 118703935 118704051 117 + 1.818 1.781 -0.124
ENSG00000145388 E023 81.8814098 0.0029619513 9.671504e-01 9.834197e-01 4 118705611 118705821 211 + 1.813 1.806 -0.022
ENSG00000145388 E024 129.2302124 0.0003066805 5.569739e-02 1.191771e-01 4 118709998 118710349 352 + 1.993 2.063 0.236
ENSG00000145388 E025 70.4853627 0.0010683010 1.210920e-02 3.379767e-02 4 118710350 118710486 137 + 1.722 1.849 0.431
ENSG00000145388 E026 287.8941404 0.0002599887 4.024904e-12 8.934919e-11 4 118710487 118715433 4947 + 2.320 2.477 0.525