ENSG00000145375

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000274008 ENSG00000145375 HEK293_OSMI2_2hA HEK293_TMG_2hB SPATA5 protein_coding protein_coding 2.264063 0.6759222 3.059208 0.06692143 0.1948546 2.16175 0.7052523 0.41221158 0.89238294 0.08036841 0.49354207 1.095774 0.38133750 0.64490000 0.27330000 -0.37160000 0.5789564938 0.0002439006 FALSE TRUE
ENST00000422835 ENSG00000145375 HEK293_OSMI2_2hA HEK293_TMG_2hB SPATA5 protein_coding processed_transcript 2.264063 0.6759222 3.059208 0.06692143 0.1948546 2.16175 0.8317663 0.05376027 1.29433123 0.02844537 0.15827196 4.354509 0.25589583 0.07236667 0.42053333 0.34816667 0.0002439006 0.0002439006 FALSE TRUE
MSTRG.25428.4 ENSG00000145375 HEK293_OSMI2_2hA HEK293_TMG_2hB SPATA5 protein_coding   2.264063 0.6759222 3.059208 0.06692143 0.1948546 2.16175 0.5590972 0.20995033 0.73179231 0.11900512 0.45166773 1.753838 0.26045833 0.28273333 0.26020000 -0.02253333 0.9512095748 0.0002439006 FALSE TRUE
MSTRG.25428.6 ENSG00000145375 HEK293_OSMI2_2hA HEK293_TMG_2hB SPATA5 protein_coding   2.264063 0.6759222 3.059208 0.06692143 0.1948546 2.16175 0.1061767 0.00000000 0.06304301 0.00000000 0.06304301 2.868746 0.06327917 0.00000000 0.02313333 0.02313333 1.0000000000 0.0002439006 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000145375 E001 0.0000000       4 122923070 122923077 8 +      
ENSG00000145375 E002 0.0000000       4 122923078 122923088 11 +      
ENSG00000145375 E003 0.5922303 0.018097897 1.000000e+00 1.000000000 4 122923089 122923100 12 + 0.187 0.201 0.130
ENSG00000145375 E004 11.8079718 0.001823556 1.858691e-01 0.308590490 4 122923101 122923234 134 + 1.090 0.967 -0.454
ENSG00000145375 E005 10.9359154 0.001773741 9.148461e-01 0.950070545 4 122923235 122923283 49 + 1.029 1.042 0.048
ENSG00000145375 E006 8.1972678 0.002046700 9.924912e-01 0.999391672 4 122923284 122923305 22 + 0.913 0.938 0.094
ENSG00000145375 E007 4.6353545 0.003688412 5.674772e-01 0.694264487 4 122927634 122927636 3 + 0.725 0.657 -0.283
ENSG00000145375 E008 13.1593605 0.002421526 4.804675e-01 0.618754531 4 122927637 122927758 122 + 1.115 1.065 -0.179
ENSG00000145375 E009 12.0552378 0.008165659 7.893625e-01 0.865171804 4 122929040 122929197 158 + 1.071 1.065 -0.021
ENSG00000145375 E010 0.2966881 0.027154185 7.666461e-01   4 122933419 122933438 20 + 0.133 0.000 -8.617
ENSG00000145375 E011 9.1889104 0.028355974 5.228327e-01 0.656576005 4 122933439 122933497 59 + 0.982 0.907 -0.282
ENSG00000145375 E012 32.2178746 0.004987476 6.395038e-02 0.133271858 4 122934097 122934738 642 + 1.503 1.390 -0.391
ENSG00000145375 E013 0.3268771 0.028614438 4.067095e-01   4 122934739 122935389 651 + 0.072 0.201 1.717
ENSG00000145375 E014 12.0927812 0.014445094 1.849281e-01 0.307399392 4 122935714 122935843 130 + 1.108 0.966 -0.523
ENSG00000145375 E015 10.0070955 0.001959752 1.263692e-01 0.229084766 4 122936100 122936156 57 + 1.036 0.874 -0.606
ENSG00000145375 E016 14.5015320 0.007338762 8.331754e-02 0.164760893 4 122938126 122938249 124 + 1.186 1.018 -0.607
ENSG00000145375 E017 18.8373905 0.001041929 6.563614e-01 0.765972672 4 122947233 122947488 256 + 1.257 1.243 -0.047
ENSG00000145375 E018 0.1451727 0.042547696 1.000000e+00   4 122952199 122952270 72 + 0.072 0.000 -7.587
ENSG00000145375 E019 11.2295096 0.001686870 7.588263e-01 0.843329807 4 122979232 122979386 155 + 1.050 1.042 -0.030
ENSG00000145375 E020 0.0000000       4 122990774 122991109 336 +      
ENSG00000145375 E021 17.5365698 0.004214350 3.946022e-02 0.090031914 4 123028186 123028395 210 + 1.265 1.086 -0.638
ENSG00000145375 E022 11.7078391 0.019492232 4.256993e-01 0.568612485 4 123056387 123056440 54 + 1.077 0.994 -0.304
ENSG00000145375 E023 13.0708942 0.034304843 9.718829e-01 0.986434287 4 123057209 123057288 80 + 1.096 1.109 0.045
ENSG00000145375 E024 15.9459223 0.013502547 9.013104e-02 0.175326578 4 123090579 123090705 127 + 1.216 1.044 -0.616
ENSG00000145375 E025 2.1055306 0.176651187 7.921502e-01 0.867061330 4 123102184 123102293 110 + 0.446 0.519 0.360
ENSG00000145375 E026 13.3895922 0.001408031 6.074656e-01 0.727431236 4 123256016 123256180 165 + 1.115 1.087 -0.102
ENSG00000145375 E027 163.8299090 0.004553081 1.333405e-06 0.000010781 4 123313888 123319433 5546 + 2.114 2.283 0.565