ENSG00000145293

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000273920 ENSG00000145293 HEK293_OSMI2_2hA HEK293_TMG_2hB ENOPH1 protein_coding protein_coding 28.37521 13.44092 47.12379 1.837203 1.505866 1.809057 21.391021 9.4855334 36.008160 1.4672964 1.0489888 1.9234035 0.74661250 0.70363333 0.76430000 0.06066667 0.4167742935 0.0003258753 FALSE TRUE
ENST00000505846 ENSG00000145293 HEK293_OSMI2_2hA HEK293_TMG_2hB ENOPH1 protein_coding protein_coding 28.37521 13.44092 47.12379 1.837203 1.505866 1.809057 3.252809 3.0470181 4.230620 0.4153354 0.8354251 0.4721501 0.12173750 0.22720000 0.08923333 -0.13796667 0.0003258753 0.0003258753 FALSE TRUE
MSTRG.25002.1 ENSG00000145293 HEK293_OSMI2_2hA HEK293_TMG_2hB ENOPH1 protein_coding   28.37521 13.44092 47.12379 1.837203 1.505866 1.809057 2.061168 0.6252493 3.506237 0.1227708 0.7232621 2.4686377 0.08773333 0.04706667 0.07543333 0.02836667 0.5436681652 0.0003258753 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000145293 E001 4.262885 0.0359982694 9.453303e-01 9.696800e-01 4 82430527 82430589 63 + 0.670 0.668 -0.009
ENSG00000145293 E002 55.175511 0.0050509352 2.085137e-04 1.000438e-03 4 82430590 82430647 58 + 1.743 1.511 -0.790
ENSG00000145293 E003 59.536542 0.0034145108 1.091928e-04 5.643194e-04 4 82430648 82430661 14 + 1.774 1.550 -0.759
ENSG00000145293 E004 190.836749 0.0010355531 2.491600e-04 1.171630e-03 4 82430662 82430884 223 + 2.251 2.152 -0.329
ENSG00000145293 E005 93.595239 0.0003693014 1.978372e-02 5.102954e-02 4 82430885 82430913 29 + 1.939 1.864 -0.255
ENSG00000145293 E006 184.816939 0.0002066312 1.630570e-04 8.050312e-04 4 82447920 82448021 102 + 2.235 2.145 -0.301
ENSG00000145293 E007 245.882993 0.0002594925 2.277458e-04 1.082297e-03 4 82451043 82451245 203 + 2.354 2.281 -0.243
ENSG00000145293 E008 303.677899 0.0001956887 5.723882e-01 6.985133e-01 4 82454722 82454854 133 + 2.427 2.439 0.040
ENSG00000145293 E009 309.032846 0.0002179730 1.414310e-01 2.499830e-01 4 82456915 82457038 124 + 2.438 2.429 -0.030
ENSG00000145293 E010 876.503822 0.0010107270 3.080403e-17 1.411984e-15 4 82459981 82461177 1197 + 2.848 2.988 0.468