ENSG00000145220

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000343470 ENSG00000145220 HEK293_OSMI2_2hA HEK293_TMG_2hB LYAR protein_coding protein_coding 58.29409 46.54492 86.58651 2.764652 4.663396 0.8953753 54.278082 38.774269 83.37739 0.7442754 4.419966 1.104358 0.91667917 0.8381000 0.9630333 0.1249333 0.005922244 0.005922244 FALSE TRUE
ENST00000452476 ENSG00000145220 HEK293_OSMI2_2hA HEK293_TMG_2hB LYAR protein_coding protein_coding 58.29409 46.54492 86.58651 2.764652 4.663396 0.8953753 1.733557 5.219949 0.00000 2.6266425 0.000000 -9.030653 0.03433333 0.1066667 0.0000000 -0.1066667 0.107372864 0.005922244 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000145220 E001 0.9963223 0.0131508595 1.383119e-03 5.251373e-03 4 4267701 4267703 3 - 0.000 0.547 11.875
ENSG00000145220 E002 1.2918645 0.0203160221 4.801861e-03 1.537899e-02 4 4267704 4267714 11 - 0.097 0.595 3.545
ENSG00000145220 E003 538.5786538 0.0017280431 1.531084e-11 3.093306e-10 4 4267715 4268023 309 - 2.650 2.818 0.560
ENSG00000145220 E004 505.7298984 0.0007057279 1.012595e-05 6.720607e-05 4 4268530 4268615 86 - 2.659 2.755 0.318
ENSG00000145220 E005 16.8604014 0.0097548652 6.352163e-03 1.953791e-02 4 4268616 4268932 317 - 1.124 1.394 0.949
ENSG00000145220 E006 461.5686453 0.0017571808 3.692678e-02 8.532030e-02 4 4273583 4273669 87 - 2.635 2.697 0.206
ENSG00000145220 E007 1065.0033245 0.0007100918 1.956115e-01 3.207319e-01 4 4274367 4274769 403 - 3.011 3.039 0.093
ENSG00000145220 E008 457.7773408 0.0024059711 3.477395e-01 4.919390e-01 4 4279447 4279530 84 - 2.663 2.644 -0.063
ENSG00000145220 E009 481.2864148 0.0002616881 6.956815e-01 7.962179e-01 4 4279642 4279749 108 - 2.670 2.687 0.055
ENSG00000145220 E010 524.4040947 0.0008138436 2.122956e-02 5.407129e-02 4 4281783 4281897 115 - 2.730 2.694 -0.120
ENSG00000145220 E011 521.3776705 0.0016931804 1.913978e-06 1.494754e-05 4 4283621 4283795 175 - 2.754 2.648 -0.351
ENSG00000145220 E012 260.7330765 0.0002304749 5.590941e-19 3.175021e-17 4 4286519 4286572 54 - 2.485 2.285 -0.668
ENSG00000145220 E013 1.1176488 0.2225863748 1.833194e-01 3.053743e-01 4 4289603 4289985 383 - 0.177 0.484 2.024
ENSG00000145220 E014 2.0388325 0.0106644415 2.630434e-01 4.009106e-01 4 4289986 4290035 50 - 0.399 0.596 0.963
ENSG00000145220 E015 218.4557730 0.0001779624 4.928791e-15 1.696792e-13 4 4290036 4290193 158 - 2.406 2.218 -0.628