ENSG00000145147

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000503823 ENSG00000145147 HEK293_OSMI2_2hA HEK293_TMG_2hB SLIT2 protein_coding protein_coding 37.63543 44.16679 32.38392 3.627377 0.6255307 -0.4475657 2.267128 0.000000 2.757133 0.0000000 0.51161715 8.1122482 0.06349167 0.0000000 0.08570000 0.08570000 3.434724e-19 5.041305e-69 FALSE TRUE
ENST00000503837 ENSG00000145147 HEK293_OSMI2_2hA HEK293_TMG_2hB SLIT2 protein_coding protein_coding 37.63543 44.16679 32.38392 3.627377 0.6255307 -0.4475657 5.414447 10.118059 1.395205 1.8100801 0.25253561 -2.8495049 0.13572500 0.2251667 0.04290000 -0.18226667 2.240885e-09 5.041305e-69 FALSE TRUE
ENST00000504154 ENSG00000145147 HEK293_OSMI2_2hA HEK293_TMG_2hB SLIT2 protein_coding protein_coding 37.63543 44.16679 32.38392 3.627377 0.6255307 -0.4475657 17.123726 17.374642 15.937653 2.4307317 0.46650264 -0.1244693 0.45987917 0.3896000 0.49260000 0.10300000 8.955047e-02 5.041305e-69 FALSE TRUE
ENST00000622093 ENSG00000145147 HEK293_OSMI2_2hA HEK293_TMG_2hB SLIT2 protein_coding protein_coding 37.63543 44.16679 32.38392 3.627377 0.6255307 -0.4475657 1.467124 3.830190 0.000000 1.3633966 0.00000000 -8.5850339 0.03609583 0.0934000 0.00000000 -0.09340000 3.028600e-08 5.041305e-69 FALSE TRUE
MSTRG.24678.11 ENSG00000145147 HEK293_OSMI2_2hA HEK293_TMG_2hB SLIT2 protein_coding   37.63543 44.16679 32.38392 3.627377 0.6255307 -0.4475657 1.348953 0.000000 2.227428 0.0000000 0.30594530 7.8056977 0.03578333 0.0000000 0.06846667 0.06846667 5.955249e-26 5.041305e-69 FALSE TRUE
MSTRG.24678.3 ENSG00000145147 HEK293_OSMI2_2hA HEK293_TMG_2hB SLIT2 protein_coding   37.63543 44.16679 32.38392 3.627377 0.6255307 -0.4475657 4.019755 5.715149 2.849598 0.3885798 0.13169371 -1.0015007 0.10479583 0.1301667 0.08796667 -0.04220000 1.513519e-02 5.041305e-69 FALSE TRUE
MSTRG.24678.7 ENSG00000145147 HEK293_OSMI2_2hA HEK293_TMG_2hB SLIT2 protein_coding   37.63543 44.16679 32.38392 3.627377 0.6255307 -0.4475657 1.695496 5.146607 0.000000 0.3323312 0.00000000 -9.0102781 0.04353333 0.1177333 0.00000000 -0.11773333 3.849087e-37 5.041305e-69 FALSE TRUE
MSTRG.24678.8 ENSG00000145147 HEK293_OSMI2_2hA HEK293_TMG_2hB SLIT2 protein_coding   37.63543 44.16679 32.38392 3.627377 0.6255307 -0.4475657 2.266769 0.000000 4.854903 0.0000000 0.07891315 8.9262673 0.06471250 0.0000000 0.14993333 0.14993333 5.041305e-69 5.041305e-69 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000145147 E001 87.6061915 0.0041684826 2.202281e-09 3.061908e-08 4 20251905 20252166 262 + 2.069 1.813 -0.861
ENSG00000145147 E002 575.7241203 0.0014375761 1.856679e-09 2.617325e-08 4 20252167 20253259 1093 + 2.805 2.704 -0.337
ENSG00000145147 E003 270.2864356 0.0001636527 8.871606e-09 1.104125e-07 4 20253260 20253563 304 + 2.473 2.377 -0.318
ENSG00000145147 E004 86.5370933 0.0003272536 2.946526e-04 1.358110e-03 4 20253564 20253589 26 + 1.990 1.882 -0.362
ENSG00000145147 E005 188.6916487 0.0002239687 3.454111e-02 8.078953e-02 4 20253590 20253815 226 + 2.277 2.252 -0.082
ENSG00000145147 E006 206.6953900 0.0002081661 1.047330e-02 2.991581e-02 4 20253816 20253994 179 + 2.320 2.287 -0.111
ENSG00000145147 E007 1.8487963 0.0089808723 3.688947e-01 5.133128e-01 4 20254920 20255075 156 + 0.514 0.381 -0.691
ENSG00000145147 E008 201.5933274 0.0003092776 1.094022e-07 1.103499e-06 4 20256672 20256743 72 + 2.354 2.248 -0.355
ENSG00000145147 E009 222.6657152 0.0003598602 3.534072e-04 1.593373e-03 4 20257868 20257939 72 + 2.368 2.310 -0.194
ENSG00000145147 E010 214.4391257 0.0001929426 2.709765e-03 9.403002e-03 4 20268810 20268881 72 + 2.342 2.299 -0.144
ENSG00000145147 E011 81.5351002 0.0052299042 7.526107e-07 6.420780e-06 4 20281429 20281821 393 + 1.680 1.992 1.054
ENSG00000145147 E012 170.9346584 0.0054440261 3.952718e-05 2.281142e-04 4 20467752 20467823 72 + 2.309 2.159 -0.501
ENSG00000145147 E013 200.5726883 0.0032273164 3.036604e-03 1.037348e-02 4 20480716 20480787 72 + 2.335 2.260 -0.250
ENSG00000145147 E014 210.4981379 0.0008650474 1.806897e-04 8.809281e-04 4 20486200 20486271 72 + 2.352 2.280 -0.240
ENSG00000145147 E015 324.0451470 0.0019750048 2.135504e-08 2.476641e-07 4 20488819 20488982 164 + 2.569 2.444 -0.418
ENSG00000145147 E016 0.0000000       4 20489943 20489954 12 +      
ENSG00000145147 E017 33.3513754 0.0006129196 6.158077e-01 7.339876e-01 4 20490803 20490814 12 + 1.515 1.515 0.000
ENSG00000145147 E018 317.9671940 0.0006895852 5.611454e-10 8.715143e-09 4 20491761 20491899 139 + 2.551 2.440 -0.370
ENSG00000145147 E019 221.7352633 0.0003041042 4.218860e-05 2.418599e-04 4 20510495 20510566 72 + 2.371 2.301 -0.235
ENSG00000145147 E020 202.8429636 0.0002170465 1.012733e-03 4.002573e-03 4 20511066 20511137 72 + 2.323 2.271 -0.175
ENSG00000145147 E021 200.6250929 0.0002041887 8.360418e-02 1.651840e-01 4 20519382 20519453 72 + 2.295 2.282 -0.043
ENSG00000145147 E022 310.4710342 0.0005189159 6.090874e-02 1.281626e-01 4 20523760 20523903 144 + 2.482 2.472 -0.031
ENSG00000145147 E023 292.2460609 0.0020139697 3.117706e-01 4.542755e-01 4 20524014 20524177 164 + 2.449 2.455 0.017
ENSG00000145147 E024 82.6595764 0.0010356530 2.215205e-01 3.522237e-01 4 20525149 20525172 24 + 1.855 1.939 0.282
ENSG00000145147 E025 309.5086071 0.0011001151 1.153827e-01 2.134095e-01 4 20528949 20529099 151 + 2.430 2.504 0.249
ENSG00000145147 E026 253.2664559 0.0004570556 2.039076e-01 3.310822e-01 4 20531984 20532058 75 + 2.350 2.414 0.214
ENSG00000145147 E027 0.0000000       4 20533399 20533571 173 +      
ENSG00000145147 E028 303.0636740 0.0001805440 2.810807e-01 4.209510e-01 4 20533572 20533715 144 + 2.432 2.487 0.185
ENSG00000145147 E029 8.7286239 0.0265229308 7.226796e-01 8.167585e-01 4 20535489 20535742 254 + 0.912 0.992 0.298
ENSG00000145147 E030 54.7600476 0.0202655189 8.281597e-07 7.000373e-06 4 20535743 20536098 356 + 1.381 1.863 1.641
ENSG00000145147 E031 178.3466798 0.0004612997 1.844308e-03 6.740722e-03 4 20539441 20539584 144 + 2.269 2.213 -0.187
ENSG00000145147 E032 210.0908191 0.0026143154 1.394256e-02 3.809257e-02 4 20541453 20541619 167 + 2.336 2.288 -0.161
ENSG00000145147 E033 181.3174549 0.0016377235 6.650536e-02 1.375643e-01 4 20542494 20542618 125 + 2.258 2.233 -0.085
ENSG00000145147 E034 74.1727180 0.0038233022 7.286513e-01 8.210605e-01 4 20542619 20542626 8 + 1.822 1.875 0.178
ENSG00000145147 E035 127.4004463 0.0006172724 6.083393e-01 7.281047e-01 4 20546031 20546099 69 + 2.079 2.094 0.050
ENSG00000145147 E036 157.0647867 0.0009247881 1.138538e-01 2.111384e-01 4 20548488 20548559 72 + 2.189 2.172 -0.058
ENSG00000145147 E037 151.0155784 0.0003653871 1.932881e-01 3.179047e-01 4 20549057 20549128 72 + 2.167 2.159 -0.025
ENSG00000145147 E038 148.6536518 0.0009730278 5.607155e-02 1.198288e-01 4 20550827 20550898 72 + 2.174 2.145 -0.099
ENSG00000145147 E039 0.2214452 0.0382660320 6.769258e-01   4 20552428 20552721 294 + 0.000 0.108 8.905
ENSG00000145147 E040 152.5132256 0.0002157341 4.804419e-03 1.538614e-02 4 20553805 20553852 48 + 2.199 2.148 -0.171
ENSG00000145147 E041 203.6859652 0.0003299798 1.047571e-01 1.977512e-01 4 20553853 20553968 116 + 2.299 2.289 -0.035
ENSG00000145147 E042 0.0000000       4 20554308 20554340 33 +      
ENSG00000145147 E043 218.8150241 0.0003089715 1.120930e-01 2.086082e-01 4 20567262 20567386 125 + 2.328 2.320 -0.027
ENSG00000145147 E044 201.0225637 0.0002037885 1.246594e-01 2.266466e-01 4 20567518 20567615 98 + 2.293 2.285 -0.026
ENSG00000145147 E045 245.6744638 0.0002635826 2.810284e-01 4.208961e-01 4 20568865 20569004 140 + 2.368 2.375 0.021
ENSG00000145147 E046 5.7241626 0.0179047066 2.161057e-02 5.486983e-02 4 20569005 20569264 260 + 0.561 0.932 1.514
ENSG00000145147 E047 0.1817044 0.0391760342 6.780395e-01   4 20573234 20573260 27 + 0.000 0.107 8.902
ENSG00000145147 E048 193.8986082 0.0014658726 8.927390e-01 9.354613e-01 4 20589644 20589737 94 + 2.253 2.283 0.098
ENSG00000145147 E049 239.5285547 0.0011839602 4.093653e-01 5.529805e-01 4 20595697 20595834 138 + 2.356 2.366 0.036
ENSG00000145147 E050 319.5394767 0.0004990230 5.357540e-01 6.675501e-01 4 20596415 20596655 241 + 2.457 2.505 0.159
ENSG00000145147 E051 216.6319222 0.0001786505 4.193152e-01 5.625602e-01 4 20598265 20598352 88 + 2.286 2.338 0.175
ENSG00000145147 E052 156.2381020 0.0002245306 2.901714e-01 4.309611e-01 4 20598353 20598395 43 + 2.139 2.202 0.212
ENSG00000145147 E053 271.2419553 0.0002802656 6.234942e-02 1.305992e-01 4 20610013 20610167 155 + 2.369 2.445 0.253
ENSG00000145147 E054 2.9830061 0.0070562494 2.195892e-01 3.498886e-01 4 20614863 20616909 2047 + 0.679 0.511 -0.750
ENSG00000145147 E055 146.8352534 0.0012801911 1.094370e-02 3.102740e-02 4 20616910 20616947 38 + 2.075 2.196 0.406
ENSG00000145147 E056 346.2401716 0.0003033259 3.734228e-02 8.611438e-02 4 20616948 20617198 251 + 2.475 2.551 0.254
ENSG00000145147 E057 333.3190568 0.0005955397 1.656866e-03 6.143879e-03 4 20617439 20617650 212 + 2.438 2.544 0.353
ENSG00000145147 E058 1526.4716282 0.0062859905 3.900201e-17 1.762178e-15 4 20618768 20620403 1636 + 2.927 3.273 1.148
ENSG00000145147 E059 150.5234094 0.0068446301 3.591777e-18 1.846544e-16 4 20620404 20620561 158 + 1.794 2.299 1.695