ENSG00000144959

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000475381 ENSG00000144959 HEK293_OSMI2_2hA HEK293_TMG_2hB NCEH1 protein_coding protein_coding 2.764373 1.399286 4.101187 0.1986086 0.2261504 1.544591 0.7862679 0.34598264 0.60315546 0.10667967 0.24604777 0.784446 0.2838750 0.23686667 0.1534000 -0.08346667 0.57332284 0.03314866 FALSE TRUE
ENST00000538775 ENSG00000144959 HEK293_OSMI2_2hA HEK293_TMG_2hB NCEH1 protein_coding protein_coding 2.764373 1.399286 4.101187 0.1986086 0.2261504 1.544591 1.1465405 0.39404896 2.41929139 0.24536307 0.19698401 2.587934 0.3378458 0.29670000 0.5914333 0.29473333 0.41652642 0.03314866 FALSE TRUE
ENST00000543711 ENSG00000144959 HEK293_OSMI2_2hA HEK293_TMG_2hB NCEH1 protein_coding protein_coding 2.764373 1.399286 4.101187 0.1986086 0.2261504 1.544591 0.4770770 0.62969444 0.05397216 0.31580320 0.05397216 -3.321867 0.3009250 0.44186667 0.0122000 -0.42966667 0.20912531 0.03314866 FALSE TRUE
MSTRG.24077.4 ENSG00000144959 HEK293_OSMI2_2hA HEK293_TMG_2hB NCEH1 protein_coding   2.764373 1.399286 4.101187 0.1986086 0.2261504 1.544591 0.3544875 0.02955962 1.02476783 0.02955962 0.30064940 4.709135 0.0773625 0.02456667 0.2429667 0.21840000 0.03314866 0.03314866 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000144959 E001 0.8480291 0.0145898965 9.182196e-04 3.678373e-03 3 172630249 172630644 396 - 0.000 0.585 12.855
ENSG00000144959 E002 194.6969129 0.0079203263 1.557921e-10 2.659909e-09 3 172630645 172633774 3130 - 2.166 2.409 0.811
ENSG00000144959 E003 17.1277408 0.0010694412 4.017383e-04 1.781182e-03 3 172633775 172633872 98 - 1.275 1.029 -0.882
ENSG00000144959 E004 18.5410201 0.0011148127 1.193230e-03 4.617960e-03 3 172633873 172633998 126 - 1.296 1.093 -0.721
ENSG00000144959 E005 17.4389701 0.0011276920 9.045127e-02 1.758358e-01 3 172633999 172634092 94 - 1.240 1.183 -0.200
ENSG00000144959 E006 20.2777682 0.0009934022 8.921018e-03 2.608319e-02 3 172635916 172636087 172 - 1.324 1.199 -0.440
ENSG00000144959 E007 0.0000000       3 172636088 172636184 97 -      
ENSG00000144959 E008 14.4800257 0.0012048483 5.253456e-03 1.662077e-02 3 172645623 172645692 70 - 1.195 1.005 -0.688
ENSG00000144959 E009 0.5954526 0.0193874923 2.155086e-01 3.450305e-01 3 172647862 172647885 24 - 0.241 0.000 -12.682
ENSG00000144959 E010 19.6495113 0.0010318853 1.036127e-04 5.386845e-04 3 172647886 172648034 149 - 1.331 1.073 -0.918
ENSG00000144959 E011 12.7614938 0.0068072626 1.721108e-01 2.909394e-01 3 172648035 172648114 80 - 1.110 1.052 -0.213
ENSG00000144959 E012 16.9406201 0.0012572729 1.559396e-02 4.183313e-02 3 172710847 172711218 372 - 1.244 1.113 -0.468