ENSG00000144895

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000460851 ENSG00000144895 HEK293_OSMI2_2hA HEK293_TMG_2hB EIF2A protein_coding protein_coding 45.77425 11.39811 80.11667 0.2876874 3.852158 2.812223 13.078541 5.8339643 20.577833 0.4162478 0.9910568 1.816773 0.36151250 0.51133333 0.25813333 -0.25320000 1.006149e-06 7.106413e-33 FALSE TRUE
ENST00000462221 ENSG00000144895 HEK293_OSMI2_2hA HEK293_TMG_2hB EIF2A protein_coding nonsense_mediated_decay 45.77425 11.39811 80.11667 0.2876874 3.852158 2.812223 12.872422 2.8442635 23.046828 0.5149091 1.5433734 3.014004 0.26723333 0.25143333 0.28720000 0.03576667 7.685046e-01 7.106413e-33 FALSE TRUE
ENST00000472926 ENSG00000144895 HEK293_OSMI2_2hA HEK293_TMG_2hB EIF2A protein_coding retained_intron 45.77425 11.39811 80.11667 0.2876874 3.852158 2.812223 2.757289 0.6987857 5.042748 0.1775922 0.3528449 2.833647 0.06463333 0.06096667 0.06286667 0.00190000 9.545260e-01 7.106413e-33   FALSE
ENST00000474505 ENSG00000144895 HEK293_OSMI2_2hA HEK293_TMG_2hB EIF2A protein_coding retained_intron 45.77425 11.39811 80.11667 0.2876874 3.852158 2.812223 3.876714 1.3509474 6.815724 0.1586140 0.7659758 2.326371 0.09115833 0.11823333 0.08473333 -0.03350000 2.432926e-01 7.106413e-33 FALSE TRUE
ENST00000482093 ENSG00000144895 HEK293_OSMI2_2hA HEK293_TMG_2hB EIF2A protein_coding protein_coding 45.77425 11.39811 80.11667 0.2876874 3.852158 2.812223 6.217361 0.0000000 11.047614 0.0000000 0.6520393 10.110824 0.08461250 0.00000000 0.13786667 0.13786667 7.106413e-33 7.106413e-33 FALSE FALSE
ENST00000494558 ENSG00000144895 HEK293_OSMI2_2hA HEK293_TMG_2hB EIF2A protein_coding nonsense_mediated_decay 45.77425 11.39811 80.11667 0.2876874 3.852158 2.812223 5.445425 0.0000000 10.910083 0.0000000 1.2847681 10.092768 0.08737083 0.00000000 0.13556667 0.13556667 2.915799e-24 7.106413e-33 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000144895 E001 0.0000000       3 150546678 150546684 7 +      
ENSG00000144895 E002 1.5500344 0.0088206145 9.249198e-01 9.567149e-01 3 150546685 150546724 40 + 0.342 0.383 0.237
ENSG00000144895 E003 3.7686879 0.0041928717 3.613717e-01 5.057594e-01 3 150546725 150546766 42 + 0.633 0.494 -0.638
ENSG00000144895 E004 9.9244847 0.0016409600 1.013749e-02 2.910182e-02 3 150546767 150546785 19 + 1.006 0.656 -1.372
ENSG00000144895 E005 9.6246761 0.0017589064 3.950632e-03 1.300433e-02 3 150546786 150546786 1 + 1.000 0.583 -1.670
ENSG00000144895 E006 10.6910325 0.0015525261 1.507049e-02 4.064836e-02 3 150546787 150546787 1 + 1.031 0.719 -1.201
ENSG00000144895 E007 11.8046184 0.0014648129 1.630252e-02 4.339558e-02 3 150546788 150546789 2 + 1.066 0.774 -1.106
ENSG00000144895 E008 12.8366186 0.0013236573 4.430265e-02 9.893576e-02 3 150546790 150546793 4 + 1.088 0.867 -0.822
ENSG00000144895 E009 24.0849612 0.0007805139 4.177198e-04 1.842338e-03 3 150546794 150546799 6 + 1.360 1.037 -1.146
ENSG00000144895 E010 118.9089649 0.0005787712 1.940889e-12 4.537771e-11 3 150546800 150546813 14 + 2.033 1.732 -1.013
ENSG00000144895 E011 144.0690538 0.0007316360 1.419048e-14 4.575126e-13 3 150546814 150546830 17 + 2.116 1.810 -1.027
ENSG00000144895 E012 0.2955422 0.0292910833 2.875303e-01   3 150547226 150547286 61 + 0.061 0.232 2.234
ENSG00000144895 E013 0.5985731 0.0230231632 7.553633e-01 8.407290e-01 3 150552354 150552355 2 + 0.161 0.232 0.649
ENSG00000144895 E014 204.5157695 0.0002839573 1.887119e-15 6.883114e-14 3 150552356 150552425 70 + 2.260 2.018 -0.810
ENSG00000144895 E015 0.4502799 0.0317191209 6.799261e-01 7.839829e-01 3 150552426 150552883 458 + 0.161 0.000 -11.769
ENSG00000144895 E016 0.1817044 0.0437299540 9.545481e-02   3 150557585 150557678 94 + 0.000 0.232 11.987
ENSG00000144895 E017 228.5318534 0.0001885668 2.761442e-17 1.272864e-15 3 150558388 150558462 75 + 2.308 2.068 -0.804
ENSG00000144895 E018 1.4767806 0.1146767633 2.254714e-01 3.569664e-01 3 150558463 150558729 267 + 0.278 0.575 1.619
ENSG00000144895 E019 124.6283173 0.0002390469 3.225218e-07 2.964475e-06 3 150562542 150562556 15 + 2.038 1.855 -0.615
ENSG00000144895 E020 240.9258047 0.0001662183 7.990090e-10 1.206646e-08 3 150562557 150562660 104 + 2.318 2.168 -0.500
ENSG00000144895 E021 0.1451727 0.0433578471 1.000000e+00   3 150562661 150562908 248 + 0.061 0.000 -10.056
ENSG00000144895 E022 238.8375260 0.0001782022 2.240567e-04 1.066504e-03 3 150563515 150563614 100 + 2.301 2.230 -0.238
ENSG00000144895 E023 179.8889426 0.0002012789 2.496874e-01 3.855482e-01 3 150564299 150564381 83 + 2.164 2.164 -0.001
ENSG00000144895 E024 40.4897481 0.0017265853 4.006648e-03 1.316054e-02 3 150564382 150564899 518 + 1.474 1.696 0.759
ENSG00000144895 E025 12.0781362 0.0030590092 1.532053e-01 2.660313e-01 3 150565191 150565226 36 + 1.055 0.907 -0.549
ENSG00000144895 E026 24.9260919 0.0007749943 7.374484e-02 1.495172e-01 3 150567399 150567692 294 + 1.345 1.219 -0.443
ENSG00000144895 E027 151.1284391 0.0002106996 6.817096e-01 7.853463e-01 3 150567693 150567766 74 + 2.085 2.108 0.077
ENSG00000144895 E028 128.0745917 0.0002463460 1.712541e-01 2.898170e-01 3 150567902 150567929 28 + 2.022 2.006 -0.055
ENSG00000144895 E029 174.1769469 0.0001972405 7.548115e-02 1.522841e-01 3 150567930 150568012 83 + 2.156 2.133 -0.075
ENSG00000144895 E030 96.0594012 0.0003376763 1.361811e-01 2.427352e-01 3 150568013 150568016 4 + 1.902 1.872 -0.102
ENSG00000144895 E031 114.5371184 0.0010441621 5.565508e-01 6.851637e-01 3 150568017 150568031 15 + 1.969 1.980 0.039
ENSG00000144895 E032 129.6262557 0.0021995910 1.706995e-01 2.890626e-01 3 150568032 150568046 15 + 2.028 2.003 -0.086
ENSG00000144895 E033 239.5327411 0.0022509692 5.468790e-01 6.769771e-01 3 150568176 150568292 117 + 2.285 2.298 0.044
ENSG00000144895 E034 670.0838319 0.0001277270 2.523583e-02 6.235933e-02 3 150571958 150572495 538 + 2.718 2.792 0.246
ENSG00000144895 E035 195.5212387 0.0013637459 1.246941e-02 3.466312e-02 3 150572496 150572529 34 + 2.173 2.296 0.413
ENSG00000144895 E036 314.8987424 0.0010682611 4.941610e-04 2.137692e-03 3 150575649 150575762 114 + 2.376 2.510 0.444
ENSG00000144895 E037 335.7939909 0.0032356955 2.301338e-06 1.766609e-05 3 150581618 150581746 129 + 2.387 2.584 0.657
ENSG00000144895 E038 257.6140848 0.0064629200 3.494692e-05 2.043455e-04 3 150583200 150583265 66 + 2.265 2.485 0.733
ENSG00000144895 E039 379.4692048 0.0041568602 4.356918e-18 2.219428e-16 3 150583846 150586016 2171 + 2.393 2.736 1.141