ENSG00000144827

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000273359 ENSG00000144827 HEK293_OSMI2_2hA HEK293_TMG_2hB ABHD10 protein_coding protein_coding 20.22926 10.63867 30.26159 2.649218 0.6539698 1.507291 17.245819 9.457951 25.326829 2.454518 0.7346510 1.420112 0.85716667 0.8866333 0.83673333 -0.04990000 3.642863e-01 1.69902e-14 FALSE  
ENST00000497293 ENSG00000144827 HEK293_OSMI2_2hA HEK293_TMG_2hB ABHD10 protein_coding retained_intron 20.22926 10.63867 30.26159 2.649218 0.6539698 1.507291 1.330624 0.000000 2.413649 0.000000 0.2391069 7.921037 0.05742083 0.0000000 0.07993333 0.07993333 1.699020e-14 1.69902e-14 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000144827 E001 5.019869 0.0031968082 3.635142e-01 5.079150e-01 3 111979010 111979017 8 + 0.742 0.721 -0.087
ENSG00000144827 E002 6.215041 0.0026755042 9.980888e-02 1.901918e-01 3 111979018 111979025 8 + 0.840 0.721 -0.474
ENSG00000144827 E003 45.138781 0.0005192222 2.880297e-04 1.331244e-03 3 111979026 111979051 26 + 1.629 1.545 -0.287
ENSG00000144827 E004 45.286030 0.0005170764 2.533148e-04 1.189071e-03 3 111979052 111979052 1 + 1.631 1.545 -0.293
ENSG00000144827 E005 144.787750 0.0003597865 2.744237e-05 1.647351e-04 3 111979053 111979203 151 + 2.110 2.108 -0.006
ENSG00000144827 E006 227.627846 0.0002982638 1.012813e-11 2.106889e-10 3 111981784 111981967 184 + 2.316 2.273 -0.141
ENSG00000144827 E007 7.228107 0.0040812601 1.121633e-06 9.216260e-06 3 111981968 111982321 354 + 0.971 0.206 -3.778
ENSG00000144827 E008 189.887547 0.0002310125 2.993843e-10 4.873053e-09 3 111986264 111986375 112 + 2.237 2.193 -0.145
ENSG00000144827 E009 187.712205 0.0009801985 1.850610e-05 1.157148e-04 3 111986914 111987051 138 + 2.221 2.223 0.006
ENSG00000144827 E010 5.922596 0.0026944877 1.721472e-04 8.444529e-04 3 111990700 111990751 52 + 0.881 0.346 -2.441
ENSG00000144827 E011 877.841229 0.0089620518 4.508405e-09 5.925104e-08 3 111991377 111993368 1992 + 2.800 3.069 0.893