ENSG00000144744

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000361055 ENSG00000144744 HEK293_OSMI2_2hA HEK293_TMG_2hB UBA3 protein_coding protein_coding 32.9217 14.56134 52.98352 2.148867 2.464292 1.862683 26.6668476 11.757283 43.114900 2.2730001 2.2051665 1.8737399 0.781008333 0.7971667 0.81343333 0.01626667 9.397390e-01 5.203431e-06 FALSE TRUE
ENST00000464605 ENSG00000144744 HEK293_OSMI2_2hA HEK293_TMG_2hB UBA3 protein_coding retained_intron 32.9217 14.56134 52.98352 2.148867 2.464292 1.862683 1.8435553 2.075955 1.371496 0.1900918 0.2268795 -0.5944764 0.097854167 0.1526000 0.02563333 -0.12696667 5.203431e-06 5.203431e-06 FALSE TRUE
ENST00000630585 ENSG00000144744 HEK293_OSMI2_2hA HEK293_TMG_2hB UBA3 protein_coding protein_coding 32.9217 14.56134 52.98352 2.148867 2.464292 1.862683 0.4322278 0.000000 3.457822 0.0000000 1.7354466 8.4378862 0.007970833 0.0000000 0.06376667 0.06376667 3.042426e-01 5.203431e-06 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000144744 E001 206.0416107 0.0041054311 3.423357e-06 2.531005e-05 3 69054730 69055002 273 - 2.209 2.401 0.640
ENSG00000144744 E002 373.1675047 0.0012126023 2.310498e-07 2.188913e-06 3 69055003 69055297 295 - 2.487 2.617 0.433
ENSG00000144744 E003 335.7069441 0.0002073797 5.871083e-06 4.115363e-05 3 69055298 69055525 228 - 2.454 2.554 0.335
ENSG00000144744 E004 184.9527330 0.0002652106 3.126486e-02 7.441838e-02 3 69055851 69055905 55 - 2.207 2.274 0.224
ENSG00000144744 E005 196.5103890 0.0002116723 2.035711e-01 3.306833e-01 3 69056000 69056063 64 - 2.238 2.278 0.134
ENSG00000144744 E006 223.4403780 0.0023047209 3.789287e-01 5.232206e-01 3 69056183 69056283 101 - 2.295 2.325 0.099
ENSG00000144744 E007 184.3879978 0.0020344883 6.252852e-01 7.414540e-01 3 69056612 69056693 82 - 2.225 2.210 -0.048
ENSG00000144744 E008 122.1407010 0.0029853278 8.872837e-01 9.319770e-01 3 69056779 69056815 37 - 2.041 2.050 0.031
ENSG00000144744 E009 127.7435069 0.0018063165 7.435771e-01 8.321916e-01 3 69057256 69057309 54 - 2.065 2.054 -0.039
ENSG00000144744 E010 7.0834369 0.0081112306 7.818540e-03 2.332487e-02 3 69061583 69061813 231 - 0.945 0.522 -1.747
ENSG00000144744 E011 181.4197646 0.0034881695 5.050669e-02 1.100594e-01 3 69061814 69061927 114 - 2.235 2.151 -0.280
ENSG00000144744 E012 176.6810899 0.0026297123 5.409037e-02 1.164051e-01 3 69062077 69062179 103 - 2.222 2.148 -0.246
ENSG00000144744 E013 181.7745803 0.0003034765 5.844158e-04 2.476089e-03 3 69062982 69063117 136 - 2.241 2.138 -0.345
ENSG00000144744 E014 68.4393627 0.0003575548 1.840872e-02 4.802894e-02 3 69063118 69063137 20 - 1.822 1.708 -0.385
ENSG00000144744 E015 128.9875718 0.0002762233 8.225155e-03 2.435535e-02 3 69063439 69063503 65 - 2.090 1.998 -0.309
ENSG00000144744 E016 135.6392922 0.0002402634 1.303164e-03 4.987484e-03 3 69064068 69064111 44 - 2.116 2.005 -0.371
ENSG00000144744 E017 152.2596673 0.0002359634 2.147975e-03 7.683966e-03 3 69067928 69068008 81 - 2.163 2.063 -0.333
ENSG00000144744 E018 1.0330470 0.0117950577 1.075070e-01 2.018581e-01 3 69071093 69071534 442 - 0.356 0.000 -11.248
ENSG00000144744 E019 154.2784995 0.0035512154 1.723798e-02 4.547228e-02 3 69071535 69071617 83 - 2.171 2.062 -0.363
ENSG00000144744 E020 0.0000000       3 69075083 69075083 1 -      
ENSG00000144744 E021 1.4749078 0.0090010759 4.029310e-02 9.157598e-02 3 69075084 69075429 346 - 0.450 0.000 -11.762
ENSG00000144744 E022 153.7866434 0.0083815105 1.811727e-01 3.026900e-01 3 69075430 69075510 81 - 2.162 2.088 -0.249
ENSG00000144744 E023 0.7374030 0.0154907848 8.732482e-01 9.225798e-01 3 69076406 69076508 103 - 0.237 0.199 -0.321
ENSG00000144744 E024 0.4438354 0.0285348621 1.239800e-01 2.256432e-01 3 69077337 69077338 2 - 0.073 0.335 2.676
ENSG00000144744 E025 1.6930144 0.1076816442 1.191444e-01 2.187159e-01 3 69077339 69077797 459 - 0.280 0.596 1.696
ENSG00000144744 E026 212.8830868 0.0066173977 5.898044e-03 1.834335e-02 3 69077798 69077918 121 - 2.315 2.185 -0.434
ENSG00000144744 E027 19.0953585 0.0399748917 1.462155e-10 2.510657e-09 3 69079744 69080111 368 - 0.888 1.654 2.712
ENSG00000144744 E028 126.7870557 0.0013886320 4.889703e-01 6.264680e-01 3 69080112 69080153 42 - 2.068 2.046 -0.072
ENSG00000144744 E029 103.1669559 0.0007039405 1.758925e-03 6.468342e-03 3 69080334 69080408 75 - 2.005 1.878 -0.428