ENSG00000144677

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000273179 ENSG00000144677 HEK293_OSMI2_2hA HEK293_TMG_2hB CTDSPL protein_coding protein_coding 18.14494 22.36068 16.22678 1.904104 0.6670144 -0.4623437 3.4044435 2.165233 3.9104198 0.18494549 0.2970229 0.8498385 0.19288333 0.09686667 0.24090000 0.14403333 9.015070e-10 2.880234e-31 FALSE TRUE
ENST00000436654 ENSG00000144677 HEK293_OSMI2_2hA HEK293_TMG_2hB CTDSPL protein_coding protein_coding 18.14494 22.36068 16.22678 1.904104 0.6670144 -0.4623437 1.6827754 1.085698 1.4820585 1.08569812 0.2235740 0.4454537 0.09399167 0.04373333 0.09103333 0.04730000 2.024994e-01 2.880234e-31 FALSE FALSE
ENST00000443503 ENSG00000144677 HEK293_OSMI2_2hA HEK293_TMG_2hB CTDSPL protein_coding protein_coding 18.14494 22.36068 16.22678 1.904104 0.6670144 -0.4623437 7.0581284 8.542426 6.3108959 1.44866725 0.1510696 -0.4362046 0.39278750 0.37926667 0.39093333 0.01166667 9.596857e-01 2.880234e-31 FALSE TRUE
ENST00000447745 ENSG00000144677 HEK293_OSMI2_2hA HEK293_TMG_2hB CTDSPL protein_coding protein_coding 18.14494 22.36068 16.22678 1.904104 0.6670144 -0.4623437 2.0950831 3.634398 1.4598835 0.57800034 0.3283588 -1.3099788 0.10912500 0.16966667 0.08866667 -0.08100000 2.572021e-01 2.880234e-31 FALSE TRUE
MSTRG.22724.12 ENSG00000144677 HEK293_OSMI2_2hA HEK293_TMG_2hB CTDSPL protein_coding   18.14494 22.36068 16.22678 1.904104 0.6670144 -0.4623437 1.3075322 1.970362 0.9921338 0.41883530 0.5109055 -0.9826887 0.07192083 0.09036667 0.06110000 -0.02926667 7.623716e-01 2.880234e-31 TRUE TRUE
MSTRG.22724.6 ENSG00000144677 HEK293_OSMI2_2hA HEK293_TMG_2hB CTDSPL protein_coding   18.14494 22.36068 16.22678 1.904104 0.6670144 -0.4623437 0.4907882 3.404935 0.0000000 0.04063392 0.0000000 -8.4157144 0.02347500 0.15436667 0.00000000 -0.15436667 2.880234e-31 2.880234e-31 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000144677 E001 4.8487725 0.0035040079 9.738048e-05 5.097663e-04 3 37861880 37861919 40 + 0.995 0.478 -2.148
ENSG00000144677 E002 5.9475966 0.0027145993 6.219234e-06 4.333426e-05 3 37861920 37861959 40 + 1.083 0.517 -2.277
ENSG00000144677 E003 5.9475966 0.0027145993 6.219234e-06 4.333426e-05 3 37861960 37862039 80 + 1.083 0.517 -2.277
ENSG00000144677 E004 3.5430781 0.0044974781 4.580033e-10 7.222187e-09 3 37862040 37862135 96 + 0.995 0.000 -16.463
ENSG00000144677 E005 1.4790604 0.0090345663 7.914097e-05 4.238429e-04 3 37862136 37862140 5 + 0.672 0.000 -15.250
ENSG00000144677 E006 2.4410423 0.0061134127 1.704410e-03 6.296724e-03 3 37862141 37862163 23 + 0.764 0.269 -2.486
ENSG00000144677 E007 2.3239822 0.0067624350 1.718895e-02 4.536002e-02 3 37862164 37862164 1 + 0.705 0.331 -1.830
ENSG00000144677 E008 2.5454274 0.0059702881 3.715148e-02 8.576521e-02 3 37862165 37862165 1 + 0.705 0.386 -1.507
ENSG00000144677 E009 2.5454274 0.0059702881 3.715148e-02 8.576521e-02 3 37862166 37862166 1 + 0.705 0.386 -1.507
ENSG00000144677 E010 3.4258236 0.0077942743 1.919775e-01 3.162460e-01 3 37862167 37862168 2 + 0.736 0.554 -0.784
ENSG00000144677 E011 3.5730726 0.0129845086 1.392087e-01 2.469767e-01 3 37862169 37862169 1 + 0.764 0.554 -0.899
ENSG00000144677 E012 4.6092506 0.0283446144 3.136625e-01 4.562813e-01 3 37862170 37862177 8 + 0.816 0.675 -0.573
ENSG00000144677 E013 26.9963845 0.0381526460 3.598209e-01 5.041304e-01 3 37862178 37862278 101 + 1.485 1.406 -0.273
ENSG00000144677 E014 0.5483223 0.0237580741 7.487585e-01 8.359020e-01 3 37898001 37898228 228 + 0.137 0.197 0.633
ENSG00000144677 E015 1.3963540 0.0091486399 4.346449e-01 5.769612e-01 3 37911673 37911827 155 + 0.455 0.331 -0.693
ENSG00000144677 E016 0.1472490 0.0440964048 3.761813e-01   3 37944714 37944804 91 + 0.137 0.000 -11.515
ENSG00000144677 E017 79.1743016 0.0013047300 3.027726e-01 4.446876e-01 3 37947057 37947211 155 + 1.914 1.889 -0.083
ENSG00000144677 E018 0.0000000       3 37954417 37954909 493 +      
ENSG00000144677 E019 0.7749789 0.0144658555 9.162592e-01 9.510644e-01 3 37954991 37955157 167 + 0.241 0.269 0.213
ENSG00000144677 E020 32.2563347 0.0008122409 8.164039e-01 8.838268e-01 3 37957111 37957143 33 + 1.496 1.530 0.117
ENSG00000144677 E021 65.9114187 0.0003740212 7.581661e-01 8.428186e-01 3 37964571 37964578 8 + 1.818 1.825 0.023
ENSG00000144677 E022 100.5336713 0.0003135859 9.751619e-01 9.885303e-01 3 37964579 37964636 58 + 1.988 2.009 0.070
ENSG00000144677 E023 89.8952788 0.0008897019 2.784389e-01 4.181218e-01 3 37964637 37964672 36 + 1.912 1.973 0.205
ENSG00000144677 E024 112.8634106 0.0009674836 3.152758e-01 4.580266e-01 3 37967826 37967882 57 + 2.015 2.068 0.180
ENSG00000144677 E025 2.8635907 0.0053980231 6.224077e-03 1.920412e-02 3 37968216 37968432 217 + 0.241 0.723 2.535
ENSG00000144677 E026 136.4833430 0.0052725549 4.851424e-01 6.230856e-01 3 37971407 37971499 93 + 2.103 2.144 0.137
ENSG00000144677 E027 127.1352182 0.0063861830 6.699535e-01 7.765772e-01 3 37975709 37975772 64 + 2.079 2.109 0.098
ENSG00000144677 E028 162.6311390 0.0002847352 2.462503e-01 3.815534e-01 3 37975773 37975894 122 + 2.175 2.228 0.175
ENSG00000144677 E029 123.6542832 0.0002866193 5.083416e-01 6.437677e-01 3 37980742 37980863 122 + 2.092 2.090 -0.004
ENSG00000144677 E030 69.6341688 0.0003810392 5.237368e-03 1.657707e-02 3 37980864 37980985 122 + 1.902 1.806 -0.325
ENSG00000144677 E031 209.4546716 0.0002149851 1.085886e-09 1.599920e-08 3 37980986 37981824 839 + 2.390 2.264 -0.423
ENSG00000144677 E032 351.6781650 0.0001586780 7.795340e-03 2.326261e-02 3 37981825 37982565 741 + 2.560 2.531 -0.096
ENSG00000144677 E033 99.0338381 0.0028849417 2.893474e-01 4.300334e-01 3 37982566 37982643 78 + 2.010 1.982 -0.093
ENSG00000144677 E034 636.4837699 0.0060018312 4.142489e-01 5.576548e-01 3 37982644 37984247 1604 + 2.767 2.813 0.153
ENSG00000144677 E035 166.2731171 0.0077211439 4.320403e-07 3.870100e-06 3 37984248 37984401 154 + 2.027 2.306 0.933
ENSG00000144677 E036 35.3166967 0.0049231009 9.191546e-08 9.393703e-07 3 37984402 37984469 68 + 1.265 1.678 1.422
ENSG00000144677 E037 0.4355181 0.6418896746 2.172004e-01 3.470076e-01 3 37984995 37985104 110 + 0.323 0.000 -11.931