ENSG00000144647

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000344697 ENSG00000144647 HEK293_OSMI2_2hA HEK293_TMG_2hB POMGNT2 protein_coding protein_coding 52.50168 78.84425 32.80896 3.490831 0.6476891 -1.264659 38.40441 47.83417 29.873586 5.996915 1.527044 -0.6789898 0.7414792 0.6030667 0.91150000 0.3084333 0.01127686 0.0001323266 FALSE TRUE
ENST00000689987 ENSG00000144647 HEK293_OSMI2_2hA HEK293_TMG_2hB POMGNT2 protein_coding protein_coding 52.50168 78.84425 32.80896 3.490831 0.6476891 -1.264659 12.54161 28.41456 1.781497 3.652551 1.781497 -3.9879005 0.2291583 0.3646333 0.05346667 -0.3111667 0.05496414 0.0001323266 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000144647 E001 0.8480291 0.0142216226 1.562410e-01 2.701069e-01 3 43079229 43079231 3 - 0.000 0.295 11.700
ENSG00000144647 E002 2.1346082 0.0781821442 1.631905e-02 4.343043e-02 3 43079232 43079233 2 - 0.000 0.542 13.508
ENSG00000144647 E003 3.0536138 0.1234989879 1.897372e-01 3.134331e-01 3 43079234 43079239 6 - 0.320 0.622 1.552
ENSG00000144647 E004 3.3481119 0.1758624184 6.211991e-01 7.381982e-01 3 43079240 43079249 10 - 0.501 0.619 0.541
ENSG00000144647 E005 15.6924040 0.0016273743 4.174104e-01 5.607701e-01 3 43079250 43079258 9 - 1.225 1.135 -0.319
ENSG00000144647 E006 27.7187302 0.0007292268 8.632207e-02 1.695151e-01 3 43079259 43079265 7 - 1.290 1.410 0.419
ENSG00000144647 E007 227.9551202 0.0006230209 6.834841e-11 1.240625e-09 3 43079266 43079374 109 - 2.133 2.320 0.626
ENSG00000144647 E008 3740.0566019 0.0005951819 5.381605e-01 6.696193e-01 3 43079375 43081536 2162 - 3.504 3.494 -0.035
ENSG00000144647 E009 6.0486209 0.0721315233 9.329113e-01 9.617254e-01 3 43085936 43086035 100 - 0.771 0.766 -0.018
ENSG00000144647 E010 5.3058253 0.0072380847 3.718372e-02 8.582186e-02 3 43086036 43089775 3740 - 0.935 0.635 -1.199
ENSG00000144647 E011 5.1628281 0.0640570225 3.103902e-01 4.527831e-01 3 43090488 43090492 5 - 0.844 0.662 -0.735
ENSG00000144647 E012 9.2456109 0.0770884750 2.310037e-01 3.636730e-01 3 43090493 43090609 117 - 1.075 0.875 -0.742
ENSG00000144647 E013 0.8814403 0.0140792629 1.556514e-01 2.692865e-01 3 43105175 43105380 206 - 0.000 0.295 12.569
ENSG00000144647 E014 0.3697384 0.0246775897 6.353878e-01 7.497123e-01 3 43105721 43105771 51 - 0.000 0.143 11.099
ENSG00000144647 E015 0.5180316 0.0223139266 3.772329e-01 5.215670e-01 3 43105772 43105835 64 - 0.000 0.200 11.707
ENSG00000144647 E016 219.1913089 0.0083357193 6.246517e-05 3.435575e-04 3 43105836 43106085 250 - 2.416 2.208 -0.693