ENSG00000144566

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000273047 ENSG00000144566 HEK293_OSMI2_2hA HEK293_TMG_2hB RAB5A protein_coding protein_coding 28.31001 12.00492 45.99016 0.5080256 1.215613 1.936812 10.552774 5.195857 17.21051 0.1074119 0.5926126 1.7259202 0.4009458 0.4350333 0.3745333 -0.0605000 4.057730e-01 3.547801e-32 FALSE TRUE
ENST00000473608 ENSG00000144566 HEK293_OSMI2_2hA HEK293_TMG_2hB RAB5A protein_coding retained_intron 28.31001 12.00492 45.99016 0.5080256 1.215613 1.936812 8.347507 6.095276 10.40529 0.8267761 0.3862358 0.7705749 0.3454750 0.5040000 0.2261333 -0.2778667 3.057915e-06 3.547801e-32 FALSE TRUE
MSTRG.22564.4 ENSG00000144566 HEK293_OSMI2_2hA HEK293_TMG_2hB RAB5A protein_coding   28.31001 12.00492 45.99016 0.5080256 1.215613 1.936812 7.219033 0.000000 15.09276 0.0000000 1.5448570 10.5605964 0.1672833 0.0000000 0.3275667 0.3275667 3.547801e-32 3.547801e-32 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000144566 E001 14.7413491 0.0011828254 4.175725e-01 5.609094e-01 3 19947097 19947249 153 + 1.136 1.131 -0.019
ENSG00000144566 E002 44.1304853 0.0017611542 8.541048e-02 1.680699e-01 3 19947250 19947378 129 + 1.596 1.565 -0.104
ENSG00000144566 E003 44.5110555 0.0004701679 2.917157e-01 4.326136e-01 3 19947379 19947398 20 + 1.590 1.607 0.058
ENSG00000144566 E004 43.1617181 0.0005182051 9.980188e-02 1.901842e-01 3 19947399 19947404 6 + 1.586 1.565 -0.073
ENSG00000144566 E005 43.7729766 0.0005541386 1.029901e-01 1.950298e-01 3 19947405 19947409 5 + 1.592 1.572 -0.068
ENSG00000144566 E006 87.6275470 0.0003228039 1.554725e-02 4.172871e-02 3 19947410 19947521 112 + 1.888 1.863 -0.083
ENSG00000144566 E007 1.9292491 0.0355957106 2.209914e-01 3.516185e-01 3 19947522 19947863 342 + 0.339 0.616 1.400
ENSG00000144566 E008 176.0236140 0.0002268503 8.159043e-08 8.431738e-07 3 19950806 19951000 195 + 2.204 2.118 -0.288
ENSG00000144566 E009 130.6597799 0.0002355593 1.455895e-06 1.167866e-05 3 19951001 19951061 61 + 2.078 1.983 -0.318
ENSG00000144566 E010 2.8192858 0.0526935189 3.805069e-01 5.247878e-01 3 19951062 19951225 164 + 0.457 0.680 1.024
ENSG00000144566 E011 5.0211183 0.0244048857 2.747976e-02 6.696800e-02 3 19970548 19970717 170 + 0.795 0.458 -1.487
ENSG00000144566 E012 0.2214452 0.0403166821 1.492135e-01   3 19975516 19975586 71 + 0.000 0.212 12.189
ENSG00000144566 E013 0.5169874 0.0202980173 1.275275e-01 2.307098e-01 3 19975587 19975600 14 + 0.068 0.353 2.888
ENSG00000144566 E014 118.0038240 0.0002793916 6.029261e-05 3.328648e-04 3 19975601 19975642 42 + 2.029 1.957 -0.242
ENSG00000144566 E015 199.6132681 0.0002169823 3.453908e-03 1.159198e-02 3 19975643 19975752 110 + 2.238 2.237 -0.004
ENSG00000144566 E016 203.0016294 0.0004996943 5.619747e-03 1.760412e-02 3 19976047 19976169 123 + 2.243 2.244 0.001
ENSG00000144566 E017 159.4458199 0.0077802827 7.115675e-02 1.452586e-01 3 19978310 19978403 94 + 2.142 2.129 -0.042
ENSG00000144566 E018 3.2203642 0.0047530498 7.959448e-02 1.589013e-01 3 19978404 19978891 488 + 0.625 0.353 -1.359
ENSG00000144566 E019 2.3679894 0.0061735958 8.553173e-01 9.105558e-01 3 19979004 19979097 94 + 0.457 0.545 0.429
ENSG00000144566 E020 0.4396707 0.0316465574 4.475615e-01 5.889364e-01 3 19983223 19983254 32 + 0.178 0.000 -10.269
ENSG00000144566 E021 798.7911439 0.0057855322 2.644505e-10 4.336480e-09 3 19983708 19985175 1468 + 2.767 3.006 0.794