ENSG00000144488

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000409506 ENSG00000144488 HEK293_OSMI2_2hA HEK293_TMG_2hB ESPNL protein_coding protein_coding 9.025397 14.78267 6.199684 1.089705 0.4462134 -1.25229 1.950518 2.434594 1.244937 0.2136792 0.5161054 -0.9619796 0.2152625 0.1663333 0.1914333 0.02510000 0.954131881 0.002782428 FALSE TRUE
ENST00000423032 ENSG00000144488 HEK293_OSMI2_2hA HEK293_TMG_2hB ESPNL protein_coding protein_coding 9.025397 14.78267 6.199684 1.089705 0.4462134 -1.25229 2.520884 3.195824 2.075203 0.3513849 0.1947380 -0.6205069 0.2917917 0.2150667 0.3337333 0.11866667 0.002782428 0.002782428 FALSE FALSE
ENST00000477241 ENSG00000144488 HEK293_OSMI2_2hA HEK293_TMG_2hB ESPNL protein_coding processed_transcript 9.025397 14.78267 6.199684 1.089705 0.4462134 -1.25229 1.133081 1.377450 0.723934 0.2181718 0.3307728 -0.9187134 0.1322167 0.0964000 0.1238667 0.02746667 0.971651665 0.002782428 FALSE TRUE
MSTRG.20131.4 ENSG00000144488 HEK293_OSMI2_2hA HEK293_TMG_2hB ESPNL protein_coding   9.025397 14.78267 6.199684 1.089705 0.4462134 -1.25229 3.410021 7.762084 2.155609 0.9475712 0.2780376 -1.8435290 0.3586292 0.5212333 0.3510000 -0.17023333 0.091451752 0.002782428 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000144488 E001 0.0000000       2 238100340 238100419 80 +      
ENSG00000144488 E002 0.0000000       2 238100420 238100713 294 +      
ENSG00000144488 E003 0.0000000       2 238101941 238102131 191 +      
ENSG00000144488 E004 0.2214452 0.0373603400 1.000000000   2 238104656 238104842 187 + 0.000 0.079 8.474
ENSG00000144488 E005 0.0000000       2 238107791 238107885 95 +      
ENSG00000144488 E006 0.1482932 0.0417594738 1.000000000   2 238107886 238107973 88 + 0.000 0.079 9.911
ENSG00000144488 E007 0.1482932 0.0417594738 1.000000000   2 238116903 238117034 132 + 0.000 0.079 9.911
ENSG00000144488 E008 0.1482932 0.0417594738 1.000000000   2 238125270 238125384 115 + 0.000 0.079 9.911
ENSG00000144488 E009 6.1360924 0.0854929581 0.291111088 0.431940356 2 238127360 238127389 30 + 0.563 0.831 1.122
ENSG00000144488 E010 6.3575376 0.1031304743 0.281782504 0.421698791 2 238127390 238127394 5 + 0.563 0.846 1.183
ENSG00000144488 E011 16.4876524 0.0046932718 0.133318354 0.238728072 2 238127395 238127453 59 + 1.229 1.140 -0.314
ENSG00000144488 E012 19.4464250 0.0009314250 0.002661228 0.009256277 2 238127454 238127528 75 + 1.377 1.186 -0.670
ENSG00000144488 E013 23.3904890 0.0009006728 0.010464088 0.029895344 2 238127529 238127621 93 + 1.414 1.273 -0.492
ENSG00000144488 E014 55.5998235 0.0033056644 0.004336150 0.014086296 2 238127622 238127734 113 + 1.750 1.647 -0.349
ENSG00000144488 E015 66.8259381 0.0156876553 0.628216814 0.743796637 2 238128707 238128904 198 + 1.742 1.763 0.073
ENSG00000144488 E016 7.6138220 0.0279158283 0.061566489 0.129269360 2 238128948 238129241 294 + 1.023 0.818 -0.778
ENSG00000144488 E017 744.7120526 0.0073453972 0.003112814 0.010597968 2 238130128 238133287 3160 + 2.724 2.823 0.331