ENSG00000144485

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000272937 ENSG00000144485 HEK293_OSMI2_2hA HEK293_TMG_2hB HES6 protein_coding protein_coding 36.4523 74.32373 14.23263 4.878498 0.1610038 -2.383801 27.538024 59.353089 9.367397 2.398151 0.3840846 -2.662307 0.7415125 0.8014667 0.6586333 -0.14283333 0.02116504 0.00317019 FALSE TRUE
ENST00000409160 ENSG00000144485 HEK293_OSMI2_2hA HEK293_TMG_2hB HES6 protein_coding protein_coding 36.4523 74.32373 14.23263 4.878498 0.1610038 -2.383801 5.593338 8.817632 2.733154 1.082228 0.2989602 -1.686191 0.1650667 0.1177333 0.1916000 0.07386667 0.02286943 0.00317019 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000144485 E001 3.4947015 0.0305754041 4.092976e-02 9.275400e-02 2 238238267 238238269 3 - 0.001 0.532 10.737
ENSG00000144485 E002 11.3766244 0.0015743260 1.336703e-02 3.677401e-02 2 238238270 238238275 6 - 0.532 0.957 1.743
ENSG00000144485 E003 571.5636366 0.0061797101 3.164911e-02 7.516678e-02 2 238238276 238238604 329 - 2.492 2.580 0.294
ENSG00000144485 E004 356.0358542 0.0023359962 2.816419e-02 6.833705e-02 2 238238605 238238655 51 - 2.296 2.374 0.261
ENSG00000144485 E005 547.8228751 0.0008928495 2.245023e-03 7.983348e-03 2 238238656 238238775 120 - 2.482 2.559 0.257
ENSG00000144485 E006 485.1631422 0.0018950328 2.911022e-03 1.000280e-02 2 238238776 238238859 84 - 2.416 2.508 0.307
ENSG00000144485 E007 655.9957083 0.0011948571 2.873052e-01 4.278009e-01 2 238238860 238239156 297 - 2.604 2.631 0.088
ENSG00000144485 E008 143.8127365 0.0053196845 5.351809e-01 6.671124e-01 2 238239157 238239169 13 - 1.999 1.966 -0.110
ENSG00000144485 E009 129.3965533 0.0063909373 5.023513e-01 6.384179e-01 2 238239170 238239179 10 - 1.959 1.920 -0.129
ENSG00000144485 E010 150.3002171 0.0002791894 8.333687e-01 8.956110e-01 2 238239180 238239215 36 - 1.981 1.987 0.022
ENSG00000144485 E011 139.9478648 0.0007280844 8.563871e-01 9.111945e-01 2 238239216 238239251 36 - 1.951 1.957 0.021
ENSG00000144485 E012 55.3578869 0.0028863045 2.221142e-04 1.058455e-03 2 238239252 238239486 235 - 1.763 1.522 -0.820
ENSG00000144485 E013 139.3882050 0.0036351980 3.047052e-02 7.288572e-02 2 238239487 238239526 40 - 2.048 1.942 -0.357
ENSG00000144485 E014 158.2124224 0.0062581814 1.480476e-02 4.005536e-02 2 238239527 238239562 36 - 2.120 1.994 -0.422
ENSG00000144485 E015 129.1831802 0.0059927401 4.703900e-02 1.038915e-01 2 238239563 238239568 6 - 2.019 1.909 -0.369
ENSG00000144485 E016 183.2650103 0.0061272143 5.700163e-05 3.165686e-04 2 238239661 238239747 87 - 2.241 2.047 -0.650
ENSG00000144485 E017 11.2611870 0.0033186028 9.038798e-06 6.070738e-05 2 238239748 238239824 77 - 1.306 0.808 -1.827
ENSG00000144485 E018 162.1411203 0.0103414903 6.463896e-05 3.541152e-04 2 238239825 238240046 222 - 2.217 1.989 -0.764
ENSG00000144485 E019 0.2214452 0.0378862907 9.949663e-01   2 238240392 238240662 271 - 0.000 0.054 8.249