ENSG00000144451

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000432529 ENSG00000144451 HEK293_OSMI2_2hA HEK293_TMG_2hB SPAG16 protein_coding protein_coding 11.85541 9.080336 17.40091 0.6389978 1.188327 0.9375861 9.2422011 8.226012 12.551936 0.6509223 0.6052349 0.609041 0.81511667 0.9069667 0.72353333 -0.18343333 2.168514e-02 5.766652e-20 FALSE TRUE
MSTRG.19899.6 ENSG00000144451 HEK293_OSMI2_2hA HEK293_TMG_2hB SPAG16 protein_coding   11.85541 9.080336 17.40091 0.6389978 1.188327 0.9375861 0.7500721 0.000000 2.034716 0.0000000 0.1030712 7.675757 0.04655833 0.0000000 0.11756667 0.11756667 5.766652e-20 5.766652e-20 FALSE TRUE
MSTRG.19899.7 ENSG00000144451 HEK293_OSMI2_2hA HEK293_TMG_2hB SPAG16 protein_coding   11.85541 9.080336 17.40091 0.6389978 1.188327 0.9375861 0.1742053 0.000000 1.174745 0.0000000 0.6387352 6.888432 0.00940000 0.0000000 0.06293333 0.06293333 2.477753e-01 5.766652e-20 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000144451 E001 0.0000000       2 213284389 213284391 3 +      
ENSG00000144451 E002 1.2962207 0.0229972416 9.978062e-01 1.000000e+00 2 213284392 213284415 24 + 0.352 0.357 0.033
ENSG00000144451 E003 1.8821083 0.0075561427 7.669384e-01 8.492961e-01 2 213284416 213284418 3 + 0.476 0.431 -0.231
ENSG00000144451 E004 2.7752931 0.0054615519 3.901432e-01 5.341543e-01 2 213284419 213284432 14 + 0.627 0.495 -0.610
ENSG00000144451 E005 2.7752931 0.0054615519 3.901432e-01 5.341543e-01 2 213284433 213284433 1 + 0.627 0.495 -0.610
ENSG00000144451 E006 4.0683933 0.0040841204 2.614301e-01 3.990781e-01 2 213284434 213284436 3 + 0.759 0.599 -0.672
ENSG00000144451 E007 5.3571373 0.0031612766 9.306307e-01 9.603622e-01 2 213284437 213284443 7 + 0.795 0.814 0.077
ENSG00000144451 E008 11.0286844 0.0015756730 2.569708e-01 3.940009e-01 2 213284444 213284454 11 + 1.121 1.015 -0.387
ENSG00000144451 E009 14.0430279 0.0013416007 1.412034e-01 2.496832e-01 2 213284455 213284463 9 + 1.223 1.096 -0.453
ENSG00000144451 E010 36.2231503 0.0006881513 7.637090e-02 1.536967e-01 2 213284464 213284480 17 + 1.601 1.505 -0.326
ENSG00000144451 E011 77.4003266 0.0005933547 1.808056e-02 4.730369e-02 2 213284481 213284619 139 + 1.922 1.836 -0.289
ENSG00000144451 E012 1.4425393 0.0096765512 3.340334e-01 4.777919e-01 2 213284620 213284751 132 + 0.439 0.267 -1.037
ENSG00000144451 E013 0.1451727 0.0431435926 7.987874e-01   2 213285886 213285918 33 + 0.097 0.000 -12.644
ENSG00000144451 E014 0.1472490 0.0455038667 7.980059e-01   2 213296040 213296063 24 + 0.097 0.000 -12.632
ENSG00000144451 E015 63.7333177 0.0007708990 1.424206e-01 2.513424e-01 2 213296064 213296110 47 + 1.828 1.772 -0.189
ENSG00000144451 E016 0.1515154 0.0434315083 7.983616e-01   2 213296111 213296128 18 + 0.097 0.000 -12.643
ENSG00000144451 E017 0.2987644 0.0277999602 3.853594e-01   2 213297022 213297139 118 + 0.176 0.000 -13.681
ENSG00000144451 E018 85.0317506 0.0003936683 7.135338e-01 8.100051e-01 2 213297262 213297357 96 + 1.931 1.926 -0.017
ENSG00000144451 E019 1.4308980 0.0112965587 8.035704e-01 8.749861e-01 2 213302513 213302582 70 + 0.397 0.357 -0.230
ENSG00000144451 E020 90.5852163 0.0003124920 8.238158e-01 8.889907e-01 2 213310059 213310177 119 + 1.957 1.957 0.002
ENSG00000144451 E021 109.2855034 0.0003705222 1.475915e-01 2.584173e-01 2 213317219 213317356 138 + 2.015 2.070 0.184
ENSG00000144451 E022 160.3385731 0.0021033183 6.377898e-07 5.525052e-06 2 213317357 213317978 622 + 2.133 2.290 0.524
ENSG00000144451 E023 4.3005389 0.0038354669 1.516358e-03 5.688957e-03 2 213318003 213318194 192 + 0.859 0.357 -2.289
ENSG00000144451 E024 5.8576951 0.0271870208 1.074414e-02 3.055414e-02 2 213340163 213340270 108 + 0.953 0.551 -1.640
ENSG00000144451 E025 0.1482932 0.0408039061 2.966670e-01   2 213343716 213343760 45 + 0.000 0.153 12.675
ENSG00000144451 E026 3.3258080 0.0045579918 5.139310e-02 1.116301e-01 2 213350528 213350645 118 + 0.739 0.431 -1.401
ENSG00000144451 E027 3.1721145 0.0053207366 3.960815e-01 5.398656e-01 2 213364076 213364145 70 + 0.676 0.550 -0.554
ENSG00000144451 E028 1.3566873 0.1364222426 9.518577e-01 9.738592e-01 2 213364146 213365418 1273 + 0.351 0.362 0.063
ENSG00000144451 E029 3.3505847 0.0048615440 3.182946e-01 4.612627e-01 2 213368814 213369051 238 + 0.698 0.550 -0.647
ENSG00000144451 E030 2.3292927 0.0107050067 7.470561e-01 8.346654e-01 2 213375010 213375119 110 + 0.543 0.494 -0.232
ENSG00000144451 E031 2.6874916 0.0160390822 8.724704e-01 9.219792e-01 2 213396507 213396662 156 + 0.573 0.550 -0.105
ENSG00000144451 E032 0.0000000       2 213422138 213422522 385 +      
ENSG00000144451 E033 0.0000000       2 213489963 213490090 128 +      
ENSG00000144451 E034 0.0000000       2 213862485 213862628 144 +      
ENSG00000144451 E035 0.0000000       2 213923863 213924004 142 +      
ENSG00000144451 E036 0.0000000       2 213929960 213930145 186 +      
ENSG00000144451 E037 0.2214452 0.0415536749 2.935905e-01   2 214013951 214014077 127 + 0.000 0.154 12.676
ENSG00000144451 E038 0.2214452 0.0415536749 2.935905e-01   2 214108196 214108261 66 + 0.000 0.154 12.676
ENSG00000144451 E039 0.0000000       2 214149140 214149266 127 +      
ENSG00000144451 E040 0.0000000       2 214238136 214241376 3241 +      
ENSG00000144451 E041 0.2214452 0.0415536749 2.935905e-01   2 214410140 214410501 362 + 0.000 0.154 12.676