ENSG00000144355

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000361609 ENSG00000144355 HEK293_OSMI2_2hA HEK293_TMG_2hB DLX1 protein_coding protein_coding 8.4845 7.939436 8.552664 0.5463743 0.3647279 0.1072072 0.7137212 0.5427884 1.2459612 0.16103373 0.1086178 1.1839927 0.08030000 0.07116667 0.1461 0.07493333 1.955359e-01 2.912562e-08 FALSE TRUE
ENST00000361725 ENSG00000144355 HEK293_OSMI2_2hA HEK293_TMG_2hB DLX1 protein_coding protein_coding 8.4845 7.939436 8.552664 0.5463743 0.3647279 0.1072072 6.1387950 5.7634548 6.4197542 0.38401224 0.1655554 0.1553287 0.72249583 0.72663333 0.7517 0.02506667 7.881934e-01 2.912562e-08 FALSE TRUE
ENST00000475989 ENSG00000144355 HEK293_OSMI2_2hA HEK293_TMG_2hB DLX1 protein_coding retained_intron 8.4845 7.939436 8.552664 0.5463743 0.3647279 0.1072072 0.4512749 0.1726013 0.5856137 0.09633494 0.1155110 1.7056803 0.05275417 0.02046667 0.0676 0.04713333 7.784341e-02 2.912562e-08   FALSE
MSTRG.19483.2 ENSG00000144355 HEK293_OSMI2_2hA HEK293_TMG_2hB DLX1 protein_coding   8.4845 7.939436 8.552664 0.5463743 0.3647279 0.1072072 0.5285523 0.8999215 0.0000000 0.32470177 0.0000000 -6.5076703 0.06543750 0.10900000 0.0000 -0.10900000 2.912562e-08 2.912562e-08 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000144355 E001 1.259788 0.2007706716 6.797911e-01 7.838716e-01 2 172084667 172084739 73 + 0.366 0.301 -0.409
ENSG00000144355 E002 1.556476 0.2091272833 3.743603e-01 5.187794e-01 2 172084740 172084748 9 + 0.475 0.301 -0.992
ENSG00000144355 E003 9.605378 0.0448937385 9.623432e-01 9.802461e-01 2 172084749 172084881 133 + 1.012 1.018 0.023
ENSG00000144355 E004 15.587165 0.0011703322 5.340191e-02 1.152088e-01 2 172085312 172085506 195 + 1.111 1.292 0.642
ENSG00000144355 E005 18.141673 0.0010460461 3.507937e-01 4.949712e-01 2 172085507 172085535 29 + 1.312 1.255 -0.201
ENSG00000144355 E006 22.694904 0.0009006728 1.906304e-02 4.947407e-02 2 172085536 172085556 21 + 1.449 1.299 -0.521
ENSG00000144355 E007 31.346232 0.0007750321 3.864033e-02 8.852269e-02 2 172085557 172085610 54 + 1.569 1.460 -0.372
ENSG00000144355 E008 22.868493 0.0086020239 1.085941e-01 2.034617e-01 2 172085611 172085618 8 + 1.439 1.324 -0.398
ENSG00000144355 E009 102.310749 0.0003106871 1.010995e-02 2.903230e-02 2 172085619 172085990 372 + 2.051 1.984 -0.224
ENSG00000144355 E010 5.488583 0.0032607730 1.250369e-03 4.810990e-03 2 172086267 172086653 387 + 0.997 0.589 -1.632
ENSG00000144355 E011 77.250043 0.0058578508 6.601652e-01 7.688629e-01 2 172086654 172086853 200 + 1.898 1.892 -0.020
ENSG00000144355 E012 10.653830 0.0175977659 8.003606e-02 1.596113e-01 2 172086854 172087138 285 + 1.153 0.958 -0.713
ENSG00000144355 E013 9.010861 0.0019594622 2.929384e-01 4.339715e-01 2 172087367 172087568 202 + 1.051 0.954 -0.357
ENSG00000144355 E014 348.431999 0.0016546069 1.906932e-06 1.490118e-05 2 172088003 172089677 1675 + 2.489 2.582 0.308