ENSG00000144224

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000272638 ENSG00000144224 HEK293_OSMI2_2hA HEK293_TMG_2hB UBXN4 protein_coding protein_coding 29.07293 8.086918 46.44695 0.4366361 0.678931 2.52045 19.1318711 4.9085064 31.4502905 0.4935568 0.96282705 2.6772398 0.61094167 0.60603333 0.67683333 0.070800000 5.239650e-01 3.919211e-07 FALSE  
ENST00000416538 ENSG00000144224 HEK293_OSMI2_2hA HEK293_TMG_2hB UBXN4 protein_coding nonsense_mediated_decay 29.07293 8.086918 46.44695 0.4366361 0.678931 2.52045 1.4907011 0.3541021 2.0638069 0.1184494 0.06238682 2.5098667 0.05223333 0.04263333 0.04440000 0.001766667 9.252114e-01 3.919211e-07 TRUE  
ENST00000426921 ENSG00000144224 HEK293_OSMI2_2hA HEK293_TMG_2hB UBXN4 protein_coding nonsense_mediated_decay 29.07293 8.086918 46.44695 0.4366361 0.678931 2.52045 2.0466963 0.0000000 3.5165763 0.0000000 0.79745125 8.4621245 0.04238333 0.00000000 0.07596667 0.075966667 3.919211e-07 3.919211e-07 TRUE  
ENST00000490163 ENSG00000144224 HEK293_OSMI2_2hA HEK293_TMG_2hB UBXN4 protein_coding processed_transcript 29.07293 8.086918 46.44695 0.4366361 0.678931 2.52045 1.1972615 0.9714979 0.8631803 0.5196073 0.24757543 -0.1687056 0.09918333 0.12070000 0.01870000 -0.102000000 6.174822e-01 3.919211e-07 FALSE  
MSTRG.19269.1 ENSG00000144224 HEK293_OSMI2_2hA HEK293_TMG_2hB UBXN4 protein_coding   29.07293 8.086918 46.44695 0.4366361 0.678931 2.52045 3.2561804 0.8041575 5.8957943 0.1489551 0.79346650 2.8587513 0.10643333 0.09923333 0.12700000 0.027766667 6.935911e-01 3.919211e-07 FALSE  
MSTRG.19269.2 ENSG00000144224 HEK293_OSMI2_2hA HEK293_TMG_2hB UBXN4 protein_coding   29.07293 8.086918 46.44695 0.4366361 0.678931 2.52045 0.8483845 0.6402716 0.9068487 0.3305478 0.17667622 0.4956412 0.04395833 0.07790000 0.01943333 -0.058466667 7.539686e-01 3.919211e-07 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000144224 E001 10.6322620 0.0015788727 1.626277e-01 0.2785779339 2 135741698 135741712 15 + 0.967 0.823 -0.551
ENSG00000144224 E002 11.2043062 0.0018235212 1.147831e-01 0.2125123105 2 135741713 135741733 21 + 0.991 0.823 -0.640
ENSG00000144224 E003 17.8914854 0.0010241841 1.399347e-01 0.2479350988 2 135741734 135741785 52 + 1.170 1.060 -0.395
ENSG00000144224 E004 46.8037889 0.0005589990 6.391419e-01 0.7525884030 2 135741786 135741854 69 + 1.544 1.572 0.094
ENSG00000144224 E005 43.7451950 0.0027197888 7.780984e-01 0.8572262435 2 135741855 135741867 13 + 1.514 1.553 0.135
ENSG00000144224 E006 126.6648203 0.0029815433 1.036943e-02 0.0296723537 2 135741868 135742011 144 + 1.987 1.918 -0.232
ENSG00000144224 E007 5.7281511 0.0058185567 3.761864e-02 0.0866156890 2 135742523 135742753 231 + 0.645 0.994 1.376
ENSG00000144224 E008 1.1093195 0.0113729318 9.326254e-01 0.9615614748 2 135747658 135747765 108 + 0.252 0.257 0.035
ENSG00000144224 E009 3.1293419 0.0359353166 8.730423e-01 0.9224034831 2 135748111 135748266 156 + 0.527 0.533 0.029
ENSG00000144224 E010 132.4620662 0.0002464838 3.401349e-03 0.0114417287 2 135748267 135748369 103 + 2.006 1.941 -0.217
ENSG00000144224 E011 78.3622637 0.0004993552 1.614780e-02 0.0430536768 2 135753539 135753567 29 + 1.783 1.708 -0.254
ENSG00000144224 E012 23.6482866 0.0008250001 6.610787e-01 0.7696193182 2 135753568 135754151 584 + 1.244 1.342 0.342
ENSG00000144224 E013 6.5720933 0.0548889418 6.625606e-01 0.7708466614 2 135754152 135754158 7 + 0.738 0.876 0.545
ENSG00000144224 E014 144.7148057 0.0002240031 7.536625e-05 0.0004060200 2 135754159 135754277 119 + 2.050 1.945 -0.351
ENSG00000144224 E015 197.4461516 0.0015484098 7.055042e-05 0.0003827591 2 135755517 135755691 175 + 2.181 2.084 -0.324
ENSG00000144224 E016 169.2241292 0.0002560655 2.115025e-04 0.0010131360 2 135761818 135761911 94 + 2.112 2.031 -0.272
ENSG00000144224 E017 120.3736467 0.0041488272 3.386061e-04 0.0015349696 2 135769769 135769823 55 + 1.976 1.833 -0.480
ENSG00000144224 E018 193.5103248 0.0014209499 1.515946e-02 0.0408487692 2 135770571 135770735 165 + 2.164 2.131 -0.110
ENSG00000144224 E019 88.8507673 0.0018551346 3.734676e-02 0.0861215691 2 135772420 135772446 27 + 1.832 1.778 -0.184
ENSG00000144224 E020 126.9099740 0.0021289760 4.404432e-01 0.5821211231 2 135772447 135772547 101 + 1.973 1.998 0.084
ENSG00000144224 E021 103.0017888 0.0034564587 1.482119e-01 0.2592473839 2 135776249 135776351 103 + 1.890 1.872 -0.060
ENSG00000144224 E022 147.6547588 0.0015974244 3.553548e-01 0.4997018994 2 135778948 135779079 132 + 2.036 2.058 0.076
ENSG00000144224 E023 0.1451727 0.0425337727 1.000000e+00   2 135780163 135780182 20 + 0.054 0.000 -7.902
ENSG00000144224 E024 222.3883826 0.0036772773 1.556664e-01 0.2693101689 2 135780183 135780385 203 + 2.216 2.222 0.019
ENSG00000144224 E025 1.1929815 0.0181066154 2.041906e-01 0.3314110897 2 135781523 135781561 39 + 0.312 0.000 -10.910
ENSG00000144224 E026 209.2439300 0.0002473380 4.548418e-01 0.5954229250 2 135782749 135782895 147 + 2.185 2.221 0.123
ENSG00000144224 E027 435.4579291 0.0001569852 3.165439e-02 0.0751765066 2 135782896 135783508 613 + 2.489 2.595 0.355
ENSG00000144224 E028 853.7196722 0.0174009145 2.689022e-04 0.0012531726 2 135783509 135785056 1548 + 2.750 2.998 0.825