ENSG00000144218

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000317233 ENSG00000144218 HEK293_OSMI2_2hA HEK293_TMG_2hB AFF3 protein_coding protein_coding 2.537321 2.365196 4.252531 0.3696357 0.4086319 0.8436638 1.17847996 0.97953184 2.2255880 0.16102373 0.03279636 1.17583633 0.47020000 0.41313333 0.53366667 0.12053333 0.3497432042 0.0004268469 FALSE TRUE
ENST00000409236 ENSG00000144218 HEK293_OSMI2_2hA HEK293_TMG_2hB AFF3 protein_coding protein_coding 2.537321 2.365196 4.252531 0.3696357 0.4086319 0.8436638 0.18776763 0.28070966 0.2916954 0.01949448 0.08048594 0.05351365 0.07510417 0.12743333 0.06743333 -0.06000000 0.3646707392 0.0004268469 FALSE TRUE
ENST00000423966 ENSG00000144218 HEK293_OSMI2_2hA HEK293_TMG_2hB AFF3 protein_coding protein_coding 2.537321 2.365196 4.252531 0.3696357 0.4086319 0.8436638 0.22320640 0.16850830 0.5508115 0.08426413 0.40664594 1.65152486 0.06820833 0.06176667 0.11646667 0.05470000 0.9601710747 0.0004268469 FALSE FALSE
ENST00000671937 ENSG00000144218 HEK293_OSMI2_2hA HEK293_TMG_2hB AFF3 protein_coding nonsense_mediated_decay 2.537321 2.365196 4.252531 0.3696357 0.4086319 0.8436638 0.51709586 0.78728623 0.6110933 0.09783630 0.01628879 -0.36028765 0.21048750 0.33693333 0.14586667 -0.19106667 0.0004268469 0.0004268469 TRUE TRUE
ENST00000672047 ENSG00000144218 HEK293_OSMI2_2hA HEK293_TMG_2hB AFF3 protein_coding processed_transcript 2.537321 2.365196 4.252531 0.3696357 0.4086319 0.8436638 0.08880476 0.05020552 0.3264344 0.05020552 0.24595760 2.48235748 0.03769583 0.01900000 0.07856667 0.05956667 0.7554620953 0.0004268469   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000144218 E001 12.4152978 0.0271258684 1.890848e-03 6.886242e-03 2 99545419 99547255 1837 - 0.901 1.344 1.599
ENSG00000144218 E002 30.0852273 0.0232043311 7.228920e-08 7.543361e-07 2 99547256 99547574 319 - 1.154 1.755 2.079
ENSG00000144218 E003 277.3112306 0.0097938200 2.149214e-10 3.579447e-09 2 99547575 99550831 3257 - 2.275 2.606 1.102
ENSG00000144218 E004 28.6592732 0.0008077625 9.744240e-01 9.880309e-01 2 99550832 99551120 289 - 1.421 1.501 0.275
ENSG00000144218 E005 38.4981774 0.0075894335 2.539085e-01 3.905382e-01 2 99551121 99551595 475 - 1.573 1.580 0.027
ENSG00000144218 E006 23.6136964 0.0105993791 1.656453e-02 4.398241e-02 2 99554311 99554486 176 - 1.417 1.289 -0.448
ENSG00000144218 E007 8.4006362 0.0019876102 5.800879e-01 7.049187e-01 2 99554487 99554534 48 - 0.901 1.038 0.511
ENSG00000144218 E008 13.3733142 0.0018813860 9.741722e-01 9.878812e-01 2 99554683 99554732 50 - 1.118 1.194 0.271
ENSG00000144218 E009 15.7036921 0.0012125288 5.998918e-02 1.265847e-01 2 99558875 99558968 94 - 1.240 1.153 -0.310
ENSG00000144218 E010 13.6208843 0.0014268296 9.135951e-01 9.492769e-01 2 99560365 99560436 72 - 1.125 1.194 0.245
ENSG00000144218 E011 15.8560949 0.0022070720 6.454476e-01 7.575463e-01 2 99565487 99565623 137 - 1.193 1.232 0.139
ENSG00000144218 E012 11.2262335 0.0132561647 1.164574e-01 2.148980e-01 2 99568852 99568915 64 - 1.103 1.000 -0.374
ENSG00000144218 E013 15.0907087 0.0302268760 3.135573e-01 4.561667e-01 2 99578327 99578451 125 - 1.199 1.170 -0.101
ENSG00000144218 E014 0.0000000       2 99580601 99580728 128 -      
ENSG00000144218 E015 19.5541506 0.0321342826 4.237151e-01 5.667574e-01 2 99582798 99582999 202 - 1.303 1.296 -0.025
ENSG00000144218 E016 17.5161061 0.0010985674 4.730696e-01 6.120772e-01 2 99587154 99587278 125 - 1.246 1.266 0.072
ENSG00000144218 E017 36.1978637 0.0006354286 6.656548e-04 2.773030e-03 2 99593195 99593836 642 - 1.593 1.467 -0.430
ENSG00000144218 E018 19.8171217 0.0023497342 1.106889e-02 3.133124e-02 2 99593837 99594289 453 - 1.340 1.207 -0.464
ENSG00000144218 E019 14.9118289 0.0119477276 6.136590e-01 7.323019e-01 2 99601435 99601621 187 - 1.167 1.197 0.107
ENSG00000144218 E020 0.0000000       2 99630578 99630682 105 -      
ENSG00000144218 E021 0.2214452 0.0382418913 2.861415e-01   2 99630916 99631015 100 - 0.000 0.168 11.192
ENSG00000144218 E022 9.0046732 0.0042984061 1.287884e-01 2.324693e-01 2 99649626 99649666 41 - 1.009 0.908 -0.376
ENSG00000144218 E023 11.9564870 0.0014610262 6.925510e-02 1.421409e-01 2 99672538 99672589 52 - 1.125 1.019 -0.386
ENSG00000144218 E024 12.4284245 0.0014366377 3.361196e-02 7.899079e-02 2 99727077 99727128 52 - 1.154 1.019 -0.488
ENSG00000144218 E025 13.1144439 0.0013663738 8.808655e-02 1.722160e-01 2 99744104 99744140 37 - 1.160 1.074 -0.311
ENSG00000144218 E026 0.0000000       2 99751737 99752220 484 -      
ENSG00000144218 E027 15.8334257 0.0039014914 1.189641e-02 3.328418e-02 2 99752221 99752301 81 - 1.262 1.107 -0.551
ENSG00000144218 E028 0.0000000       2 99776569 99776708 140 -      
ENSG00000144218 E029 0.0000000       2 99777237 99777929 693 -      
ENSG00000144218 E030 0.1515154 0.0429272734 8.060539e-01   2 99825495 99825936 442 - 0.088 0.000 -10.492
ENSG00000144218 E031 9.7651975 0.0062731506 6.851726e-02 1.409151e-01 2 99837477 99837524 48 - 1.063 0.932 -0.484
ENSG00000144218 E032 0.2955422 0.0305934587 6.899544e-01   2 100006149 100006631 483 - 0.088 0.168 1.070
ENSG00000144218 E033 29.5913682 0.0008040798 6.900034e-03 2.096470e-02 2 100006632 100007017 386 - 1.499 1.399 -0.342
ENSG00000144218 E034 8.2441159 0.0084048972 2.727266e-01 4.116845e-01 2 100007148 100007151 4 - 0.968 0.908 -0.228
ENSG00000144218 E035 15.5874262 0.0011811092 8.182414e-02 1.624240e-01 2 100007152 100007212 61 - 1.229 1.153 -0.270
ENSG00000144218 E036 13.2269385 0.0023639116 7.850556e-02 1.570932e-01 2 100007213 100007248 36 - 1.167 1.074 -0.335
ENSG00000144218 E037 10.8791126 0.0086232443 1.067268e-02 3.038909e-02 2 100007249 100007278 30 - 1.125 0.908 -0.802
ENSG00000144218 E038 9.2442729 0.0037185318 6.303311e-03 1.941034e-02 2 100007279 100007292 14 - 1.071 0.824 -0.928
ENSG00000144218 E039 11.6555761 0.0015575996 8.003176e-03 2.378728e-02 2 100007293 100007336 44 - 1.154 0.955 -0.723
ENSG00000144218 E040 7.7729250 0.0026571529 9.492595e-02 1.827682e-01 2 100007337 100007363 27 - 0.979 0.853 -0.476
ENSG00000144218 E041 5.6808965 0.0029179817 2.203230e-01 3.508025e-01 2 100007364 100007364 1 - 0.849 0.757 -0.362
ENSG00000144218 E042 8.7265644 0.0020260581 1.186131e-02 3.319825e-02 2 100007365 100007398 34 - 1.045 0.824 -0.835
ENSG00000144218 E043 11.9291455 0.0017393962 4.645712e-04 2.024326e-03 2 100007399 100007460 62 - 1.180 0.881 -1.097
ENSG00000144218 E044 0.0000000       2 100007461 100007951 491 -      
ENSG00000144218 E045 12.4494715 0.0018789216 1.658788e-05 1.047997e-04 2 100008812 100008856 45 - 1.217 0.824 -1.451
ENSG00000144218 E046 10.2037626 0.0019047067 4.076702e-04 1.803851e-03 2 100008857 100008886 30 - 1.125 0.792 -1.249
ENSG00000144218 E047 7.6906255 0.0287339658 1.331002e-02 3.664169e-02 2 100008887 100008898 12 - 1.008 0.721 -1.110
ENSG00000144218 E048 9.0117340 0.0031352236 2.908938e-01 4.317023e-01 2 100008899 100008932 34 - 0.999 0.956 -0.161
ENSG00000144218 E049 2.7626182 0.0055179323 3.848943e-01 5.290463e-01 2 100011497 100011571 75 - 0.480 0.679 0.902
ENSG00000144218 E050 0.6558305 0.0381957449 5.984773e-01 7.200587e-01 2 100063126 100063249 124 - 0.161 0.289 1.074
ENSG00000144218 E051 9.6601765 0.0269928219 6.584591e-01 7.675979e-01 2 100104402 100104513 112 - 1.008 1.020 0.043
ENSG00000144218 E052 3.9293975 0.1327753033 9.503103e-01 9.729013e-01 2 100104514 100104518 5 - 0.661 0.688 0.115
ENSG00000144218 E053 0.1817044 0.0389007726 2.872190e-01   2 100104519 100104580 62 - 0.000 0.168 11.186
ENSG00000144218 E054 3.0876093 0.1374986216 5.426811e-01 6.733910e-01 2 100104668 100104716 49 - 0.617 0.535 -0.370
ENSG00000144218 E055 0.1451727 0.0424484655 8.067969e-01   2 100105195 100105334 140 - 0.088 0.000 -10.494
ENSG00000144218 E056 0.5181333 0.0202660680 9.238605e-01 9.560524e-01 2 100105335 100105503 169 - 0.161 0.168 0.074
ENSG00000144218 E057 0.1482932 0.0430957717 2.871036e-01   2 100105504 100105583 80 - 0.000 0.168 11.158
ENSG00000144218 E058 0.0000000       2 100105584 100105671 88 -      
ENSG00000144218 E059 0.0000000       2 100105672 100105716 45 -      
ENSG00000144218 E060 0.0000000       2 100105717 100105779 63 -      
ENSG00000144218 E061 0.0000000       2 100105780 100106039 260 -      
ENSG00000144218 E062 0.0000000       2 100106040 100106128 89 -      
ENSG00000144218 E063 0.0000000       2 100109845 100110333 489 -      
ENSG00000144218 E064 0.0000000       2 100112386 100112524 139 -      
ENSG00000144218 E065 0.0000000       2 100129224 100129306 83 -      
ENSG00000144218 E066 0.0000000       2 100137574 100137577 4 -      
ENSG00000144218 E067 0.0000000       2 100137578 100137937 360 -      
ENSG00000144218 E068 0.0000000       2 100142484 100142595 112 -      
ENSG00000144218 E069 0.0000000       2 100142596 100142710 115 -      
ENSG00000144218 E070 0.0000000       2 100142711 100142788 78 -      
ENSG00000144218 E071 0.0000000       2 100192142 100192428 287 -