ENSG00000144021

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000272402 ENSG00000144021 HEK293_OSMI2_2hA HEK293_TMG_2hB CIAO1 protein_coding retained_intron 69.35324 84.23952 62.30559 5.552251 0.4461202 -0.4350753 7.668243 3.75373 9.711735 0.1680032 0.1188845 1.3690508 0.1198208 0.04476667 0.1558667 0.11110000 1.746164e-33 1.746164e-33   FALSE
ENST00000488633 ENSG00000144021 HEK293_OSMI2_2hA HEK293_TMG_2hB CIAO1 protein_coding protein_coding 69.35324 84.23952 62.30559 5.552251 0.4461202 -0.4350753 42.838314 57.12476 37.787615 0.5687424 0.1151488 -0.5960734 0.6148417 0.68526667 0.6065333 -0.07873333 5.355724e-01 1.746164e-33 FALSE TRUE
ENST00000491394 ENSG00000144021 HEK293_OSMI2_2hA HEK293_TMG_2hB CIAO1 protein_coding retained_intron 69.35324 84.23952 62.30559 5.552251 0.4461202 -0.4350753 15.206337 17.68507 11.890551 4.6096538 0.4692887 -0.5723187 0.2151125 0.20433333 0.1907333 -0.01360000 9.925460e-01 1.746164e-33 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000144021 E001 10.158294 0.0250001805 3.173420e-02 7.533393e-02 2 96266159 96266224 66 + 0.806 1.062 0.963
ENSG00000144021 E002 9.113611 0.0177657363 6.362815e-02 1.327405e-01 2 96266225 96266231 7 + 0.806 1.012 0.780
ENSG00000144021 E003 23.010663 0.0343619380 2.601938e-01 3.976712e-01 2 96266232 96266240 9 + 1.269 1.362 0.326
ENSG00000144021 E004 27.731830 0.0465515158 2.519433e-01 3.882584e-01 2 96266241 96266245 5 + 1.328 1.447 0.413
ENSG00000144021 E005 631.495092 0.0056438956 4.144405e-02 9.370839e-02 2 96266246 96266489 244 + 2.740 2.773 0.110
ENSG00000144021 E006 7.685442 0.0343014990 8.211758e-02 1.628657e-01 2 96267290 96267320 31 + 1.072 0.794 -1.049
ENSG00000144021 E007 967.409096 0.0007426895 5.988581e-04 2.529190e-03 2 96267321 96267469 149 + 2.940 2.950 0.035
ENSG00000144021 E008 1108.754370 0.0003680688 8.034379e-06 5.453366e-05 2 96267625 96267736 112 + 2.997 3.011 0.045
ENSG00000144021 E009 49.369033 0.0041921026 5.848742e-05 3.239181e-04 2 96267737 96267835 99 + 1.839 1.556 -0.960
ENSG00000144021 E010 1067.087897 0.0009397969 5.333299e-11 9.852536e-10 2 96267836 96267924 89 + 2.942 3.011 0.230
ENSG00000144021 E011 14.650880 0.0476992097 6.597231e-04 2.751284e-03 2 96267925 96267979 55 + 1.440 0.927 -1.833
ENSG00000144021 E012 1636.092930 0.0010397881 1.274068e-16 5.411847e-15 2 96268457 96268658 202 + 3.113 3.201 0.292
ENSG00000144021 E013 253.301668 0.0001949419 2.631598e-98 7.669974e-95 2 96268659 96269267 609 + 2.660 2.133 -1.757
ENSG00000144021 E014 1043.347193 0.0007768590 1.903294e-26 2.347483e-24 2 96269268 96269355 88 + 2.881 3.018 0.453
ENSG00000144021 E015 3591.826600 0.0044439144 1.010418e-10 1.781485e-09 2 96271111 96274173 3063 + 3.640 3.453 -0.624