ENSG00000143994

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000416071 ENSG00000143994 HEK293_OSMI2_2hA HEK293_TMG_2hB ABHD1 protein_coding protein_coding 0.9882409 1.846239 0.7831208 0.3845862 0.1068578 -1.22677 0.04658369 0.11588038 0.1050281 0.115880385 0.10502805 -0.1300677 0.04990417 0.04436667 0.1402333 0.09586667 0.9473425067 0.0007961201 FALSE FALSE
ENST00000420647 ENSG00000143994 HEK293_OSMI2_2hA HEK293_TMG_2hB ABHD1 protein_coding nonsense_mediated_decay 0.9882409 1.846239 0.7831208 0.3845862 0.1068578 -1.22677 0.37841399 0.65940453 0.3940219 0.144696260 0.03491693 -0.7284450 0.38511250 0.35730000 0.5121667 0.15486667 0.2659175429 0.0007961201 TRUE FALSE
ENST00000448950 ENSG00000143994 HEK293_OSMI2_2hA HEK293_TMG_2hB ABHD1 protein_coding nonsense_mediated_decay 0.9882409 1.846239 0.7831208 0.3845862 0.1068578 -1.22677 0.05385932 0.00000000 0.0000000 0.000000000 0.00000000 0.0000000 0.05415417 0.00000000 0.0000000 0.00000000   0.0007961201 TRUE TRUE
ENST00000489120 ENSG00000143994 HEK293_OSMI2_2hA HEK293_TMG_2hB ABHD1 protein_coding retained_intron 0.9882409 1.846239 0.7831208 0.3845862 0.1068578 -1.22677 0.13498783 0.10931848 0.1776708 0.022588586 0.11117360 0.6533864 0.14812083 0.05963333 0.2110000 0.15136667 0.5226134202 0.0007961201 TRUE TRUE
ENST00000496739 ENSG00000143994 HEK293_OSMI2_2hA HEK293_TMG_2hB ABHD1 protein_coding retained_intron 0.9882409 1.846239 0.7831208 0.3845862 0.1068578 -1.22677 0.07862552 0.31602751 0.0000000 0.202374997 0.00000000 -5.0269218 0.07512917 0.14886667 0.0000000 -0.14886667 0.2195657294 0.0007961201   FALSE
ENST00000621324 ENSG00000143994 HEK293_OSMI2_2hA HEK293_TMG_2hB ABHD1 protein_coding protein_coding 0.9882409 1.846239 0.7831208 0.3845862 0.1068578 -1.22677 0.01421656 0.09481174 0.0000000 0.094811739 0.00000000 -3.3897284 0.01343333 0.06243333 0.0000000 -0.06243333 0.9218666721 0.0007961201 FALSE TRUE
ENST00000622011 ENSG00000143994 HEK293_OSMI2_2hA HEK293_TMG_2hB ABHD1 protein_coding retained_intron 0.9882409 1.846239 0.7831208 0.3845862 0.1068578 -1.22677 0.10234564 0.17549129 0.1064002 0.046609591 0.01608471 -0.6722583 0.11471250 0.10123333 0.1365667 0.03533333 0.7785347817 0.0007961201 FALSE FALSE
MSTRG.18107.8 ENSG00000143994 HEK293_OSMI2_2hA HEK293_TMG_2hB ABHD1 protein_coding   0.9882409 1.846239 0.7831208 0.3845862 0.1068578 -1.22677 0.14429961 0.32232333 0.0000000 0.009737126 0.00000000 -5.0545157 0.13652500 0.19010000 0.0000000 -0.19010000 0.0007961201 0.0007961201 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000143994 E001 0.4031496 0.025070476 0.62433776 0.74067591 2 27123789 27123814 26 + 0.000 0.148 9.366
ENSG00000143994 E002 0.4031496 0.025070476 0.62433776 0.74067591 2 27123815 27123822 8 + 0.000 0.148 10.991
ENSG00000143994 E003 0.4031496 0.025070476 0.62433776 0.74067591 2 27123823 27123835 13 + 0.000 0.148 10.991
ENSG00000143994 E004 0.7728880 0.016329565 0.22725009 0.35911553 2 27123836 27123839 4 + 0.000 0.258 11.844
ENSG00000143994 E005 3.4935727 0.004913228 0.77636722 0.85598478 2 27123840 27123854 15 + 0.558 0.606 0.216
ENSG00000143994 E006 9.0852099 0.002146932 0.57529524 0.70092776 2 27123855 27123955 101 + 0.892 0.959 0.252
ENSG00000143994 E007 12.7293807 0.001560783 0.87904896 0.92632169 2 27123956 27124062 107 + 1.059 1.077 0.064
ENSG00000143994 E008 13.8049122 0.004760386 0.79892041 0.87169887 2 27124063 27126961 2899 + 1.132 1.111 -0.076
ENSG00000143994 E009 0.5503986 0.020059425 0.83046630 0.89364257 2 27128441 27128601 161 + 0.183 0.148 -0.367
ENSG00000143994 E010 6.1552300 0.098339440 0.01684145 0.04458085 2 27128945 27129127 183 + 1.040 0.660 -1.481
ENSG00000143994 E011 1.4477361 0.010814646 0.01621925 0.04320792 2 27129128 27129315 188 + 0.617 0.206 -2.370
ENSG00000143994 E012 2.1315749 0.007193553 0.09235338 0.17879222 2 27129316 27129361 46 + 0.183 0.509 2.090
ENSG00000143994 E013 6.5528034 0.003141244 0.25518798 0.39202243 2 27129514 27129626 113 + 0.920 0.783 -0.533
ENSG00000143994 E014 10.3042236 0.001882613 0.45798013 0.59831161 2 27129754 27129927 174 + 0.920 1.005 0.316
ENSG00000143994 E015 0.3697384 0.028001384 0.62319064 0.73982240 2 27130105 27130114 10 + 0.000 0.148 10.988
ENSG00000143994 E016 0.4782907 0.023359678 0.36816270 0.51256526 2 27130115 27130153 39 + 0.000 0.206 11.489
ENSG00000143994 E017 0.4782907 0.023359678 0.36816270 0.51256526 2 27130154 27130226 73 + 0.000 0.206 11.489
ENSG00000143994 E018 5.9634650 0.003256284 0.49487136 0.63178112 2 27130227 27130250 24 + 0.715 0.811 0.385
ENSG00000143994 E019 10.4418216 0.001883337 0.77112935 0.85224975 2 27130251 27130416 166 + 0.972 1.005 0.122
ENSG00000143994 E020 7.7313934 0.028789341 0.67937476 0.78358088 2 27130533 27130812 280 + 0.829 0.898 0.263