ENSG00000143952

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000272322 ENSG00000143952 HEK293_OSMI2_2hA HEK293_TMG_2hB VPS54 protein_coding protein_coding 9.178196 3.630814 13.55969 0.08331356 0.2894257 1.898055 2.6359913 1.0792152 3.6553973 0.1754316 0.3485183 1.7506805 0.26550833 0.2995667 0.26920000 -0.03036667 8.929931e-01 3.56406e-08 FALSE TRUE
ENST00000409558 ENSG00000143952 HEK293_OSMI2_2hA HEK293_TMG_2hB VPS54 protein_coding protein_coding 9.178196 3.630814 13.55969 0.08331356 0.2894257 1.898055 1.6180587 1.0521689 2.3788364 0.1650379 0.3972133 1.1692948 0.21529167 0.2895667 0.17680000 -0.11276667 2.426231e-01 3.56406e-08 FALSE TRUE
ENST00000416400 ENSG00000143952 HEK293_OSMI2_2hA HEK293_TMG_2hB VPS54 protein_coding nonsense_mediated_decay 9.178196 3.630814 13.55969 0.08331356 0.2894257 1.898055 1.2527012 1.2724342 0.8588592 0.3439171 0.2502517 -0.5616905 0.22173333 0.3473667 0.06416667 -0.28320000 3.490067e-03 3.56406e-08 TRUE TRUE
MSTRG.18514.3 ENSG00000143952 HEK293_OSMI2_2hA HEK293_TMG_2hB VPS54 protein_coding   9.178196 3.630814 13.55969 0.08331356 0.2894257 1.898055 0.8681136 0.0000000 1.8593943 0.0000000 0.3921857 7.5464271 0.07428750 0.0000000 0.13763333 0.13763333 7.354380e-07 3.56406e-08 FALSE TRUE
MSTRG.18514.5 ENSG00000143952 HEK293_OSMI2_2hA HEK293_TMG_2hB VPS54 protein_coding   9.178196 3.630814 13.55969 0.08331356 0.2894257 1.898055 1.4311532 0.0000000 2.7385177 0.0000000 0.6211333 8.1025099 0.10747500 0.0000000 0.20020000 0.20020000 3.564060e-08 3.56406e-08 FALSE TRUE
MSTRG.18514.7 ENSG00000143952 HEK293_OSMI2_2hA HEK293_TMG_2hB VPS54 protein_coding   9.178196 3.630814 13.55969 0.08331356 0.2894257 1.898055 1.0580896 0.1245286 1.8186180 0.1245286 0.3134453 3.7647686 0.08230833 0.0348000 0.13323333 0.09843333 1.864699e-01 3.56406e-08 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000143952 E001 0.5869198 0.1769632664 4.607783e-01 6.007658e-01 2 63892146 63892149 4 - 0.217 0.001 -8.194
ENSG00000143952 E002 67.2277562 0.0217791673 4.101119e-06 2.974406e-05 2 63892150 63892900 751 - 1.637 2.029 1.324
ENSG00000143952 E003 30.1340328 0.0006837633 1.000405e-04 5.220322e-04 2 63892901 63893040 140 - 1.337 1.611 0.942
ENSG00000143952 E004 22.0045470 0.0008423829 1.553669e-04 7.709420e-04 2 63893041 63893051 11 - 1.197 1.501 1.058
ENSG00000143952 E005 25.5351108 0.0008029799 3.207820e-05 1.894433e-04 2 63893052 63893071 20 - 1.256 1.567 1.078
ENSG00000143952 E006 173.9287023 0.0002908740 1.497373e-12 3.564178e-11 2 63893072 63893535 464 - 2.109 2.321 0.708
ENSG00000143952 E007 74.6626818 0.0003936064 5.353135e-02 1.154289e-01 2 63897496 63897590 95 - 1.781 1.878 0.328
ENSG00000143952 E008 67.0710348 0.0003561848 3.318446e-01 4.754724e-01 2 63899474 63899581 108 - 1.744 1.800 0.189
ENSG00000143952 E009 62.4244331 0.0003837453 7.502992e-02 1.515783e-01 2 63912345 63912425 81 - 1.748 1.657 -0.307
ENSG00000143952 E010 65.0495107 0.0008563787 9.336006e-01 9.621690e-01 2 63912540 63912661 122 - 1.745 1.757 0.040
ENSG00000143952 E011 45.5633770 0.0010197554 4.824359e-02 1.060660e-01 2 63913223 63913310 88 - 1.562 1.688 0.426
ENSG00000143952 E012 1.1854928 0.0222963881 5.761567e-01 7.016434e-01 2 63913825 63913925 101 - 0.329 0.218 -0.798
ENSG00000143952 E013 46.1677284 0.0005206255 1.150958e-01 2.129808e-01 2 63914182 63914287 106 - 1.576 1.676 0.341
ENSG00000143952 E014 41.5670306 0.0005720943 1.919668e-01 3.162382e-01 2 63916900 63916963 64 - 1.536 1.625 0.302
ENSG00000143952 E015 49.1021818 0.0004979832 8.089412e-02 1.609363e-01 2 63919883 63919995 113 - 1.597 1.705 0.365
ENSG00000143952 E016 68.3405139 0.0009513118 5.947409e-01 7.169337e-01 2 63920446 63920627 182 - 1.756 1.791 0.117
ENSG00000143952 E017 54.2698799 0.0031809981 2.526070e-01 3.889848e-01 2 63921206 63921335 130 - 1.646 1.723 0.260
ENSG00000143952 E018 98.2061956 0.0002937919 8.921905e-01 9.351219e-01 2 63933673 63934013 341 - 1.919 1.921 0.006
ENSG00000143952 E019 50.4104680 0.0004988211 1.188832e-01 2.183450e-01 2 63942465 63942561 97 - 1.656 1.567 -0.300
ENSG00000143952 E020 40.0451997 0.0005025696 6.808113e-03 2.072430e-02 2 63944600 63944655 56 - 1.576 1.390 -0.639
ENSG00000143952 E021 48.0081937 0.0004534416 1.431150e-04 7.170207e-04 2 63947383 63947490 108 - 1.662 1.412 -0.854
ENSG00000143952 E022 50.1882115 0.0004643708 6.503709e-05 3.560903e-04 2 63949037 63949163 127 - 1.681 1.423 -0.883
ENSG00000143952 E023 113.0678527 0.0003582449 2.431399e-10 4.013080e-09 2 63962058 63962443 386 - 2.030 1.753 -0.934
ENSG00000143952 E024 37.5679170 0.0005882821 3.337372e-03 1.125490e-02 2 63965835 63965875 41 - 1.553 1.341 -0.730
ENSG00000143952 E025 48.9115563 0.0004862562 9.799149e-04 3.891212e-03 2 63965876 63965966 91 - 1.663 1.454 -0.715
ENSG00000143952 E026 32.4338688 0.0116918854 6.968351e-03 2.114175e-02 2 63968957 63968991 35 - 1.496 1.241 -0.885
ENSG00000143952 E027 37.1439696 0.0294227469 1.041863e-01 1.968738e-01 2 63972166 63972244 79 - 1.538 1.374 -0.567
ENSG00000143952 E028 0.0000000       2 63974986 63975602 617 -      
ENSG00000143952 E029 35.1247522 0.0053622529 1.013909e-01 1.925828e-01 2 63981646 63981690 45 - 1.505 1.380 -0.430
ENSG00000143952 E030 50.9444363 0.0004389851 2.753281e-03 9.531481e-03 2 63981691 63981845 155 - 1.675 1.492 -0.625
ENSG00000143952 E031 21.8617626 0.0037506889 2.214217e-02 5.597352e-02 2 63981846 63981851 6 - 1.328 1.106 -0.785
ENSG00000143952 E032 12.1513628 0.0019034024 5.879125e-01 7.111642e-01 2 63981852 63981887 36 - 1.062 1.006 -0.204
ENSG00000143952 E033 42.6594902 0.0005307227 1.485841e-03 5.590629e-03 2 63983864 63984019 156 - 1.606 1.390 -0.743
ENSG00000143952 E034 36.3655114 0.0286945606 4.803043e-04 2.084634e-03 2 64018938 64019428 491 - 1.563 1.154 -1.423