ENSG00000143756

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000366862 ENSG00000143756 HEK293_OSMI2_2hA HEK293_TMG_2hB FBXO28 protein_coding protein_coding 14.14904 6.96964 24.48581 0.7229855 0.2779185 1.81131 9.2290863 5.0094364 16.2341666 0.7790866 0.6659756 1.694324 0.68510000 0.71243333 0.66260000 -0.04983333 6.444453e-01 2.892371e-07 FALSE TRUE
ENST00000424254 ENSG00000143756 HEK293_OSMI2_2hA HEK293_TMG_2hB FBXO28 protein_coding protein_coding 14.14904 6.96964 24.48581 0.7229855 0.2779185 1.81131 1.5760418 0.1933025 2.0649024 0.1271838 0.4491428 3.351344 0.08146250 0.02973333 0.08390000 0.05416667 3.645576e-01 2.892371e-07 FALSE TRUE
ENST00000483773 ENSG00000143756 HEK293_OSMI2_2hA HEK293_TMG_2hB FBXO28 protein_coding retained_intron 14.14904 6.96964 24.48581 0.7229855 0.2779185 1.81131 0.6876496 0.8038915 0.9545483 0.1961835 0.2775671 0.245017 0.06841667 0.12236667 0.03923333 -0.08313333 1.266900e-01 2.892371e-07 FALSE TRUE
ENST00000523990 ENSG00000143756 HEK293_OSMI2_2hA HEK293_TMG_2hB FBXO28 protein_coding nonsense_mediated_decay 14.14904 6.96964 24.48581 0.7229855 0.2779185 1.81131 1.0609362 0.0191032 3.0490482 0.0191032 0.6382497 6.715761 0.05842500 0.00230000 0.12490000 0.12260000 2.892371e-07 2.892371e-07 FALSE TRUE
MSTRG.3112.4 ENSG00000143756 HEK293_OSMI2_2hA HEK293_TMG_2hB FBXO28 protein_coding   14.14904 6.96964 24.48581 0.7229855 0.2779185 1.81131 1.4030396 0.7501776 2.0621697 0.0984994 0.1410446 1.446734 0.08494583 0.10763333 0.08426667 -0.02336667 5.640702e-01 2.892371e-07 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000143756 E001 6.1645610 0.0988899452 9.317311e-01 9.609979e-01 1 224114111 224114118 8 + 0.789 0.823 0.137
ENSG00000143756 E002 35.3665242 0.0051354185 7.444086e-01 8.327732e-01 1 224114119 224114170 52 + 1.484 1.551 0.230
ENSG00000143756 E003 49.8410764 0.0020610087 4.135711e-01 5.570353e-01 1 224114171 224114217 47 + 1.649 1.646 -0.010
ENSG00000143756 E004 121.9743442 0.0028029728 1.266606e-02 3.512475e-02 1 224114218 224114396 179 + 2.046 1.977 -0.230
ENSG00000143756 E005 126.2926752 0.0067312996 5.847642e-02 1.240250e-01 1 224130472 224130581 110 + 2.059 1.996 -0.211
ENSG00000143756 E006 0.2924217 0.0274283517 8.369271e-01   1 224130582 224131021 440 + 0.128 0.001 -7.399
ENSG00000143756 E007 126.8165228 0.0003048656 2.316863e-02 5.810007e-02 1 224134074 224134212 139 + 2.055 2.020 -0.118
ENSG00000143756 E008 4.0137824 0.0064473876 4.984208e-06 3.548453e-05 1 224134213 224134365 153 + 0.342 1.037 3.045
ENSG00000143756 E009 152.0969684 0.0002092101 4.511634e-05 2.569723e-04 1 224153142 224153337 196 + 2.147 2.057 -0.300
ENSG00000143756 E010 206.1288453 0.0015350308 1.956045e-03 7.089745e-03 1 224157352 224157570 219 + 2.269 2.210 -0.196
ENSG00000143756 E011 308.0656448 0.0040194035 1.438733e-01 2.532836e-01 1 224157571 224158022 452 + 2.432 2.419 -0.043
ENSG00000143756 E012 753.4929818 0.0015115768 2.900063e-01 4.307665e-01 1 224158023 224160388 2366 + 2.811 2.838 0.092
ENSG00000143756 E013 461.5153353 0.0099879265 9.393070e-06 6.283146e-05 1 224160389 224162047 1659 + 2.531 2.772 0.803