ENSG00000143753

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000323699 ENSG00000143753 HEK293_OSMI2_2hA HEK293_TMG_2hB DEGS1 protein_coding protein_coding 126.5029 91.87883 182.6328 11.82611 3.621485 0.9910632 104.29739 84.167429 147.34119 11.0015255 2.805717 0.8077533 0.8357875 0.9160333 0.8071 -0.1089333 0.0002209452 0.0002209452 FALSE TRUE
MSTRG.3113.4 ENSG00000143753 HEK293_OSMI2_2hA HEK293_TMG_2hB DEGS1 protein_coding   126.5029 91.87883 182.6328 11.82611 3.621485 0.9910632 15.25465 6.357689 24.09431 0.7841777 2.663861 1.9204495 0.1153208 0.0698000 0.1322 0.0624000 0.0128806985 0.0002209452 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000143753 E001 0.3030308 0.4114229889 4.867607e-01   1 224165879 224165919 41 + 0.183 0.000 -9.185
ENSG00000143753 E002 0.0000000       1 224175756 224175947 192 +      
ENSG00000143753 E003 367.5828606 0.0050550148 2.762566e-04 1.283059e-03 1 224183240 224183265 26 + 2.625 2.470 -0.519
ENSG00000143753 E004 771.3027962 0.0050966765 1.051889e-03 4.139018e-03 1 224183266 224183418 153 + 2.938 2.806 -0.440
ENSG00000143753 E005 2.7508526 0.1308383000 7.337916e-01 8.249636e-01 1 224183675 224183831 157 + 0.614 0.532 -0.371
ENSG00000143753 E006 2810.0881100 0.0004977545 8.514613e-08 8.761912e-07 1 224189577 224190135 559 + 3.473 3.412 -0.204
ENSG00000143753 E007 1772.6048456 0.0006031344 1.636920e-01 2.799430e-01 1 224190136 224190319 184 + 3.258 3.235 -0.075
ENSG00000143753 E008 31.5739930 0.0044994201 6.029213e-02 1.271089e-01 1 224191092 224191156 65 + 1.456 1.587 0.449
ENSG00000143753 E009 1738.2665281 0.0014055653 2.378772e-01 3.716712e-01 1 224192332 224192518 187 + 3.232 3.247 0.050
ENSG00000143753 E010 1716.8283504 0.0005665987 1.166659e-04 5.985973e-04 1 224192519 224192822 304 + 3.214 3.260 0.154
ENSG00000143753 E011 613.3609004 0.0019808220 6.757304e-04 2.810368e-03 1 224192823 224192895 73 + 2.751 2.833 0.272
ENSG00000143753 E012 960.8158634 0.0016463270 6.096995e-12 1.314208e-10 1 224192896 224193441 546 + 2.914 3.058 0.476
ENSG00000143753 E013 10.9432896 0.0031416090 5.145393e-03 1.632495e-02 1 224200963 224201163 201 + 0.945 1.226 1.021