ENSG00000143751

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000272091 ENSG00000143751 HEK293_OSMI2_2hA HEK293_TMG_2hB SDE2 protein_coding protein_coding 21.46729 12.32348 36.70007 4.044785 1.473958 1.573596 15.024957 11.93093 23.82539 4.017589 0.6998149 0.99719 0.7681833 0.9641667 0.6505333 -0.3136333 2.713232e-09 5.207322e-43 FALSE TRUE
MSTRG.3147.4 ENSG00000143751 HEK293_OSMI2_2hA HEK293_TMG_2hB SDE2 protein_coding   21.46729 12.32348 36.70007 4.044785 1.473958 1.573596 6.176469 0.00000 12.71650 0.000000 1.1721435 10.31362 0.2155708 0.0000000 0.3452000 0.3452000 5.207322e-43 5.207322e-43 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000143751 E001 0.0000000       1 225956402 225956541 140 -      
ENSG00000143751 E002 21.8324150 0.0010549355 2.132793e-22 1.777612e-20 1 225982702 225982770 69 - 0.900 1.709 2.855
ENSG00000143751 E003 97.2312367 0.0123084198 8.524735e-14 2.447318e-12 1 225982771 225982880 110 - 1.683 2.268 1.964
ENSG00000143751 E004 93.1770694 0.0088180268 5.285456e-17 2.348297e-15 1 225982881 225982926 46 - 1.668 2.251 1.962
ENSG00000143751 E005 1012.5579548 0.0099541388 1.924047e-03 6.990351e-03 1 225982927 225984996 2070 - 2.919 3.090 0.570
ENSG00000143751 E006 373.1117044 0.0072073208 1.465512e-02 3.971632e-02 1 225984997 225985523 527 - 2.579 2.508 -0.237
ENSG00000143751 E007 0.8815316 0.0136968632 5.786706e-02 1.229751e-01 1 225987892 225987895 4 - 0.362 0.000 -10.677
ENSG00000143751 E008 498.3000196 0.0056139585 2.158191e-03 7.714685e-03 1 225987896 225988388 493 - 2.706 2.628 -0.260
ENSG00000143751 E009 182.0748519 0.0107004289 1.386877e-03 5.264057e-03 1 225991243 225991363 121 - 2.292 2.141 -0.506
ENSG00000143751 E010 210.2361395 0.0047387480 1.514402e-03 5.682630e-03 1 225992398 225992567 170 - 2.338 2.245 -0.309
ENSG00000143751 E011 160.4122503 0.0053331697 1.073673e-03 4.213547e-03 1 225992891 225993002 112 - 2.226 2.115 -0.373
ENSG00000143751 E012 179.3544202 0.0002528441 4.735268e-14 1.410285e-12 1 225995266 225995383 118 - 2.289 2.139 -0.501
ENSG00000143751 E013 120.2787172 0.0007606944 3.497557e-11 6.659983e-10 1 225999193 225999379 187 - 2.124 1.941 -0.613