ENSG00000143727

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000272065 ENSG00000143727 HEK293_OSMI2_2hA HEK293_TMG_2hB ACP1 protein_coding protein_coding 84.21586 64.52143 110.7148 8.736943 3.421707 0.7789051 53.50988 44.477496 66.04956 7.2977934 3.304785 0.5703676 0.6371708 0.6845000 0.5960000 -0.0885000000 0.04682225 6.478931e-05 FALSE  
ENST00000272067 ENSG00000143727 HEK293_OSMI2_2hA HEK293_TMG_2hB ACP1 protein_coding protein_coding 84.21586 64.52143 110.7148 8.736943 3.421707 0.7789051 13.22095 9.593489 20.68121 1.1099242 1.128404 1.1073873 0.1538875 0.1495000 0.1865667 0.0370666667 0.04052359 6.478931e-05 FALSE  
ENST00000413140 ENSG00000143727 HEK293_OSMI2_2hA HEK293_TMG_2hB ACP1 protein_coding nonsense_mediated_decay 84.21586 64.52143 110.7148 8.736943 3.421707 0.7789051 10.05274 7.697882 13.43000 0.5715094 1.317761 0.8021262 0.1218042 0.1227667 0.1219333 -0.0008333333 1.00000000 6.478931e-05 TRUE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000143727 E001 0.0000000       2 264140 264498 359 +      
ENSG00000143727 E002 0.5879639 0.0218261113 5.316221e-01 6.640482e-01 2 264723 264868 146 + 0.252 0.147 -0.967
ENSG00000143727 E003 3.5587797 0.0043791403 1.577171e-01 2.720867e-01 2 264869 264892 24 + 0.737 0.534 -0.884
ENSG00000143727 E004 5.0461694 0.0178218074 2.480366e-01 3.836183e-01 2 264893 264899 7 + 0.848 0.700 -0.591
ENSG00000143727 E005 34.9328160 0.0006191073 2.197276e-01 3.500575e-01 2 264900 264915 16 + 1.580 1.522 -0.199
ENSG00000143727 E006 38.4788205 0.0005286330 2.800299e-01 4.198313e-01 2 264916 264918 3 + 1.617 1.572 -0.156
ENSG00000143727 E007 47.5872475 0.0004775465 3.979517e-01 5.416898e-01 2 264919 264925 7 + 1.700 1.672 -0.095
ENSG00000143727 E008 62.0643423 0.0004911892 2.767830e-01 4.162619e-01 2 264926 264935 10 + 1.817 1.783 -0.115
ENSG00000143727 E009 115.0232166 0.0014934358 3.646600e-02 8.447098e-02 2 264936 264946 11 + 2.092 2.024 -0.227
ENSG00000143727 E010 201.7557176 0.0017395704 4.979555e-08 5.370996e-07 2 264947 264964 18 + 2.371 2.205 -0.556
ENSG00000143727 E011 395.1397165 0.0017262234 7.803529e-04 3.188928e-03 2 264965 265007 43 + 2.630 2.549 -0.269
ENSG00000143727 E012 1.1532381 0.0184883151 8.881962e-01 9.325413e-01 2 265205 265278 74 + 0.312 0.345 0.204
ENSG00000143727 E013 0.7696683 0.0165656082 9.880294e-01 9.965616e-01 2 269301 269347 47 + 0.252 0.257 0.034
ENSG00000143727 E014 538.9550928 0.0001769772 2.454589e-07 2.312585e-06 2 271866 271939 74 + 2.761 2.693 -0.225
ENSG00000143727 E015 384.2946758 0.0002240779 6.414600e-04 2.685524e-03 2 272037 272065 29 + 2.608 2.558 -0.166
ENSG00000143727 E016 482.5998660 0.0001208499 2.438889e-01 3.787618e-01 2 272066 272150 85 + 2.671 2.705 0.114
ENSG00000143727 E017 3.8008395 0.0069941771 1.006352e-02 2.891620e-02 2 272151 272191 41 + 0.814 0.417 -1.773
ENSG00000143727 E018 144.6248166 0.0003511989 3.481985e-08 3.871194e-07 2 272192 272305 114 + 2.225 2.069 -0.524
ENSG00000143727 E019 21.6968578 0.0008495780 1.634398e-14 5.225855e-13 2 272306 273155 850 + 1.539 0.872 -2.381
ENSG00000143727 E020 16.2656921 0.0028125242 6.453555e-08 6.801705e-07 2 274573 275139 567 + 1.392 0.873 -1.872
ENSG00000143727 E021 524.3000988 0.0006128615 2.701030e-01 4.086848e-01 2 275140 275201 62 + 2.707 2.742 0.115
ENSG00000143727 E022 11.1162264 0.0018926352 2.024896e-04 9.745210e-04 2 275202 275611 410 + 1.210 0.823 -1.431
ENSG00000143727 E023 660.3444709 0.0017654156 7.287745e-03 2.196969e-02 2 276980 277085 106 + 2.787 2.862 0.250
ENSG00000143727 E024 2134.5168075 0.0003188856 1.987645e-15 7.223352e-14 2 277227 278283 1057 + 3.294 3.373 0.260