ENSG00000143674

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000366622 ENSG00000143674 HEK293_OSMI2_2hA HEK293_TMG_2hB MAP3K21 protein_coding protein_coding 10.25412 6.681876 13.3906 0.5978044 0.4236208 1.001815 2.300017 3.7077001 1.0023543 0.57325344 0.17676128 -1.876696 0.29251250 0.55110000 0.07430000 -0.47680000 6.915624e-16 6.915624e-16 FALSE TRUE
ENST00000366623 ENSG00000143674 HEK293_OSMI2_2hA HEK293_TMG_2hB MAP3K21 protein_coding protein_coding 10.25412 6.681876 13.3906 0.5978044 0.4236208 1.001815 1.063947 0.5644190 1.2776980 0.03627912 0.06255135 1.164619 0.10654583 0.08666667 0.09556667 0.00890000 8.107273e-01 6.915624e-16 FALSE TRUE
ENST00000366624 ENSG00000143674 HEK293_OSMI2_2hA HEK293_TMG_2hB MAP3K21 protein_coding protein_coding 10.25412 6.681876 13.3906 0.5978044 0.4236208 1.001815 1.436272 0.2012361 3.8603242 0.20123613 0.27610261 4.195526 0.12277917 0.03653333 0.28783333 0.25130000 4.735632e-02 6.915624e-16 FALSE TRUE
MSTRG.3291.3 ENSG00000143674 HEK293_OSMI2_2hA HEK293_TMG_2hB MAP3K21 protein_coding   10.25412 6.681876 13.3906 0.5978044 0.4236208 1.001815 0.914460 0.4647407 0.6432783 0.23401085 0.28439226 0.460558 0.07789167 0.06723333 0.04730000 -0.01993333 8.531350e-01 6.915624e-16 FALSE TRUE
MSTRG.3291.4 ENSG00000143674 HEK293_OSMI2_2hA HEK293_TMG_2hB MAP3K21 protein_coding   10.25412 6.681876 13.3906 0.5978044 0.4236208 1.001815 4.539423 1.7437804 6.6069473 0.29444449 0.44515574 1.915698 0.40029167 0.25846667 0.49503333 0.23656667 1.769707e-03 6.915624e-16 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000143674 E001 5.584204 0.0033799967 7.631273e-04 3.125786e-03 1 233327724 233327767 44 + 0.863 0.434 -1.875
ENSG00000143674 E002 48.003764 0.0410204224 5.443164e-05 3.038993e-04 1 233327768 233328665 898 + 1.715 1.382 -1.143
ENSG00000143674 E003 8.855353 0.0023386744 8.304038e-03 2.455515e-02 1 233328666 233328733 68 + 0.995 0.789 -0.786
ENSG00000143674 E004 18.407225 0.0010887707 3.846651e-05 2.226509e-04 1 233328734 233328833 100 + 1.302 1.053 -0.889
ENSG00000143674 E005 42.566589 0.0120276171 5.464663e-03 1.719450e-02 1 233346442 233346622 181 + 1.614 1.529 -0.290
ENSG00000143674 E006 57.144907 0.0070209609 3.172342e-04 1.449638e-03 1 233353807 233353955 149 + 1.741 1.645 -0.323
ENSG00000143674 E007 71.344534 0.0274659664 1.442866e-02 3.919679e-02 1 233354836 233355011 176 + 1.830 1.756 -0.250
ENSG00000143674 E008 77.857276 0.0080829158 8.821666e-05 4.666047e-04 1 233362053 233362293 241 + 1.873 1.773 -0.337
ENSG00000143674 E009 61.473239 0.0003675550 4.679952e-07 4.163823e-06 1 233372038 233372160 123 + 1.779 1.670 -0.368
ENSG00000143674 E010 16.195234 0.0011470989 9.639589e-01 9.812693e-01 1 233372161 233372638 478 + 1.151 1.286 0.478
ENSG00000143674 E011 7.921795 0.0022506056 9.124158e-01 9.484833e-01 1 233372639 233372782 144 + 0.863 1.007 0.540
ENSG00000143674 E012 37.546058 0.0022245743 2.073730e-02 5.303211e-02 1 233372783 233374134 1352 + 1.445 1.729 0.969
ENSG00000143674 E013 4.309429 0.0043570248 1.173212e-01 2.161274e-01 1 233375783 233375915 133 + 0.555 0.896 1.406
ENSG00000143674 E014 72.153372 0.0010431428 1.521385e-04 7.564480e-04 1 233375916 233376066 151 + 1.828 1.794 -0.114
ENSG00000143674 E015 56.414466 0.0007694537 4.839922e-02 1.063292e-01 1 233376430 233376527 98 + 1.699 1.743 0.148
ENSG00000143674 E016 194.068451 0.0002592556 7.253732e-10 1.104572e-08 1 233378931 233379710 780 + 2.250 2.230 -0.067
ENSG00000143674 E017 730.100588 0.0174666140 2.414627e-07 2.278430e-06 1 233382305 233385148 2844 + 2.699 3.025 1.085