ENSG00000143622

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000368322 ENSG00000143622 HEK293_OSMI2_2hA HEK293_TMG_2hB RIT1 protein_coding protein_coding 12.95615 10.36737 14.91461 1.607385 0.5058412 0.524252 2.8145742 2.9560395 3.2547640 0.39572116 0.60028961 0.1384409 0.21485417 0.28763333 0.21953333 -0.06810000 5.230680e-01 6.298319e-09 FALSE TRUE
ENST00000368323 ENSG00000143622 HEK293_OSMI2_2hA HEK293_TMG_2hB RIT1 protein_coding protein_coding 12.95615 10.36737 14.91461 1.607385 0.5058412 0.524252 3.1923418 2.0866642 4.2127541 0.39882715 0.02533233 1.0100884 0.24247500 0.20013333 0.28310000 0.08296667 4.297173e-02 6.298319e-09 FALSE TRUE
ENST00000461050 ENSG00000143622 HEK293_OSMI2_2hA HEK293_TMG_2hB RIT1 protein_coding nonsense_mediated_decay 12.95615 10.36737 14.91461 1.607385 0.5058412 0.524252 0.3040091 1.3135846 0.0000000 0.65681379 0.00000000 -7.0483066 0.02584167 0.13220000 0.00000000 -0.13220000 1.222940e-01 6.298319e-09 FALSE FALSE
ENST00000539040 ENSG00000143622 HEK293_OSMI2_2hA HEK293_TMG_2hB RIT1 protein_coding protein_coding 12.95615 10.36737 14.91461 1.607385 0.5058412 0.524252 3.2716070 2.6305333 3.1778564 0.99359609 0.38938084 0.2717573 0.26512500 0.23616667 0.21183333 -0.02433333 9.536785e-01 6.298319e-09 FALSE TRUE
ENST00000609492 ENSG00000143622 HEK293_OSMI2_2hA HEK293_TMG_2hB RIT1 protein_coding protein_coding 12.95615 10.36737 14.91461 1.607385 0.5058412 0.524252 1.1391557 0.4431729 1.3999514 0.21348567 0.30225890 1.6375120 0.08830000 0.05080000 0.09403333 0.04323333 4.589533e-01 6.298319e-09 FALSE FALSE
ENST00000650659 ENSG00000143622 HEK293_OSMI2_2hA HEK293_TMG_2hB RIT1 protein_coding retained_intron 12.95615 10.36737 14.91461 1.607385 0.5058412 0.524252 0.7249637 0.4676823 0.1977795 0.46768234 0.19777946 -1.2009985 0.06292500 0.04773333 0.01246667 -0.03526667 9.814939e-01 6.298319e-09   FALSE
ENST00000651853 ENSG00000143622 HEK293_OSMI2_2hA HEK293_TMG_2hB RIT1 protein_coding protein_coding 12.95615 10.36737 14.91461 1.607385 0.5058412 0.524252 1.3319196 0.4696929 2.6353912 0.07181241 0.24797387 2.4632980 0.08763750 0.04530000 0.17643333 0.13113333 6.298319e-09 6.298319e-09 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000143622 E001 67.8464957 0.0003683451 2.092330e-02 5.342772e-02 1 155897808 155898836 1029 - 1.889 1.789 -0.337
ENSG00000143622 E002 172.7603638 0.0007558461 1.458491e-13 4.050824e-12 1 155898837 155900182 1346 - 2.331 2.122 -0.700
ENSG00000143622 E003 49.8468443 0.0004595165 2.320268e-02 5.816806e-02 1 155900183 155900273 91 - 1.663 1.770 0.360
ENSG00000143622 E004 73.2528576 0.0004643095 8.391008e-04 3.398579e-03 1 155900274 155900397 124 - 1.817 1.947 0.439
ENSG00000143622 E005 115.6008697 0.0004277183 5.468932e-05 3.051401e-04 1 155900398 155900593 196 - 2.015 2.139 0.416
ENSG00000143622 E006 52.4140555 0.0011299832 9.468665e-02 1.824146e-01 1 155900594 155900618 25 - 1.699 1.777 0.265
ENSG00000143622 E007 72.5774649 0.0004081856 1.694309e-01 2.874506e-01 1 155904311 155904373 63 - 1.851 1.903 0.175
ENSG00000143622 E008 92.0281328 0.0002973119 8.886928e-01 9.328217e-01 1 155904374 155904502 129 - 1.975 1.977 0.004
ENSG00000143622 E009 0.4417471 0.6681730681 4.145041e-01 5.579192e-01 1 155904603 155904605 3 - 0.264 0.000 -11.137
ENSG00000143622 E010 0.4417471 0.6681730681 4.145041e-01 5.579192e-01 1 155904606 155904610 5 - 0.264 0.000 -11.137
ENSG00000143622 E011 0.5932625 0.1458391480 1.079403e-01 2.024751e-01 1 155904612 155904730 119 - 0.326 0.000 -11.554
ENSG00000143622 E012 70.4483860 0.0009871836 8.865403e-01 9.314389e-01 1 155904731 155904804 74 - 1.860 1.860 0.000
ENSG00000143622 E013 0.5138669 0.0216310810 7.827887e-01 8.605389e-01 1 155910095 155910449 355 - 0.193 0.140 -0.562
ENSG00000143622 E014 59.0728783 0.0004833271 8.247478e-01 8.895961e-01 1 155910450 155910506 57 - 1.781 1.786 0.019
ENSG00000143622 E015 80.9114138 0.0004001282 2.196823e-01 3.500060e-01 1 155910656 155910804 149 - 1.898 1.941 0.143
ENSG00000143622 E016 2.9173308 0.0061476409 2.132851e-01 3.424082e-01 1 155910805 155910877 73 - 0.666 0.462 -0.942
ENSG00000143622 E017 3.4238254 0.0057835455 1.816283e-02 4.748046e-02 1 155910878 155910915 38 - 0.781 0.400 -1.733
ENSG00000143622 E018 42.2518886 0.0005693440 9.457366e-01 9.699461e-01 1 155911243 155911271 29 - 1.647 1.641 -0.022
ENSG00000143622 E019 50.1012325 0.0004448771 9.760205e-01 9.890270e-01 1 155911272 155911404 133 - 1.715 1.713 -0.007