ENSG00000143515

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000368489 ENSG00000143515 HEK293_OSMI2_2hA HEK293_TMG_2hB ATP8B2 protein_coding protein_coding 31.94253 32.89833 31.40659 0.231285 1.443167 -0.06692638 13.651880 7.901924 20.463479 2.672270 2.000772 1.371656 0.42749167 0.2404667 0.6575000 0.4170333 0.01737079 0.0008416595 FALSE TRUE
ENST00000505882 ENSG00000143515 HEK293_OSMI2_2hA HEK293_TMG_2hB ATP8B2 protein_coding retained_intron 31.94253 32.89833 31.40659 0.231285 1.443167 -0.06692638 1.725276 3.926659 0.000000 2.374456 0.000000 -8.620828 0.05295833 0.1187333 0.0000000 -0.1187333 0.16570385 0.0008416595 FALSE TRUE
ENST00000672630 ENSG00000143515 HEK293_OSMI2_2hA HEK293_TMG_2hB ATP8B2 protein_coding protein_coding 31.94253 32.89833 31.40659 0.231285 1.443167 -0.06692638 13.119346 17.753146 7.447685 1.408989 2.116380 -1.252087 0.41142917 0.5400000 0.2328667 -0.3071333 0.01973453 0.0008416595 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000143515 E001 0.1515154 0.0430253352 4.739419e-01   1 154325525 154325534 10 + 0.120 0.000 -9.479
ENSG00000143515 E002 3.7771201 0.0041442429 4.875694e-05 2.754845e-04 1 154325535 154325542 8 + 0.902 0.301 -2.805
ENSG00000143515 E003 10.3720274 0.0086260865 8.747475e-07 7.354144e-06 1 154325543 154325552 10 + 1.259 0.728 -1.983
ENSG00000143515 E004 21.9413934 0.1400224084 8.772260e-03 2.571413e-02 1 154325553 154325604 52 + 1.527 1.126 -1.402
ENSG00000143515 E005 48.0132295 0.0215305640 8.974215e-04 3.605272e-03 1 154325605 154325702 98 + 1.803 1.556 -0.838
ENSG00000143515 E006 0.0000000       1 154327800 154327861 62 +      
ENSG00000143515 E007 76.6459380 0.0071666911 2.721081e-04 1.266275e-03 1 154328105 154328172 68 + 1.970 1.800 -0.574
ENSG00000143515 E008 0.0000000       1 154328799 154328799 1 +      
ENSG00000143515 E009 0.2987644 0.0269677742 1.913174e-01   1 154328800 154329075 276 + 0.214 0.000 -10.486
ENSG00000143515 E010 2.5486485 0.0154576561 3.002853e-01 4.419526e-01 1 154330325 154330395 71 + 0.621 0.477 -0.667
ENSG00000143515 E011 97.4990715 0.0038674883 9.833568e-05 5.141272e-04 1 154330396 154330454 59 + 2.063 1.917 -0.490
ENSG00000143515 E012 1.1093195 0.0113655514 4.468519e-02 9.962547e-02 1 154330455 154330702 248 + 0.463 0.125 -2.514
ENSG00000143515 E013 129.9792471 0.0023554051 7.258371e-04 2.991834e-03 1 154330815 154330880 66 + 2.162 2.067 -0.317
ENSG00000143515 E014 129.4210646 0.0040940161 2.165582e-02 5.496189e-02 1 154330881 154330928 48 + 2.144 2.083 -0.207
ENSG00000143515 E015 208.7582939 0.0018044139 3.909962e-06 2.849183e-05 1 154331048 154331146 99 + 2.373 2.264 -0.364
ENSG00000143515 E016 162.4459393 0.0076853325 2.078826e-02 5.314235e-02 1 154331444 154331486 43 + 2.251 2.174 -0.255
ENSG00000143515 E017 115.8250268 0.0107055381 1.022873e-01 1.939994e-01 1 154331487 154331505 19 + 2.096 2.038 -0.195
ENSG00000143515 E018 3.9187387 0.1285524634 3.814099e-01 5.257117e-01 1 154331506 154331605 100 + 0.784 0.598 -0.778
ENSG00000143515 E019 164.9364318 0.0092669513 9.102921e-03 2.654089e-02 1 154331606 154331678 73 + 2.269 2.169 -0.336
ENSG00000143515 E020 175.4122253 0.0008897758 5.022455e-08 5.414803e-07 1 154331954 154332024 71 + 2.306 2.179 -0.425
ENSG00000143515 E021 189.1725954 0.0031022573 7.223578e-07 6.185803e-06 1 154332618 154332697 80 + 2.348 2.201 -0.489
ENSG00000143515 E022 242.4241500 0.0001882367 5.510613e-10 8.573286e-09 1 154334107 154334265 159 + 2.437 2.330 -0.358
ENSG00000143515 E023 201.0496745 0.0001932860 1.540850e-06 1.228368e-05 1 154334503 154334591 89 + 2.347 2.262 -0.284
ENSG00000143515 E024 286.4588123 0.0014209003 1.739013e-04 8.522853e-04 1 154337348 154337544 197 + 2.490 2.424 -0.220
ENSG00000143515 E025 1.6390069 0.0357866632 3.282577e-02 7.746027e-02 1 154337545 154337846 302 + 0.587 0.221 -2.107
ENSG00000143515 E026 0.3268771 0.0294990551 9.798413e-01   1 154340537 154340543 7 + 0.120 0.125 0.071
ENSG00000143515 E027 310.4738957 0.0001952618 5.384172e-09 6.983536e-08 1 154340854 154341062 209 + 2.534 2.450 -0.280
ENSG00000143515 E028 4.2146948 0.0167599158 3.132187e-01 4.557687e-01 1 154341167 154341788 622 + 0.761 0.639 -0.507
ENSG00000143515 E029 162.2675680 0.0027114362 3.303754e-03 1.116000e-02 1 154342480 154342523 44 + 2.250 2.179 -0.239
ENSG00000143515 E030 260.4849216 0.0012252344 8.918726e-02 1.738907e-01 1 154342796 154342961 166 + 2.421 2.412 -0.030
ENSG00000143515 E031 304.1093512 0.0002470733 1.012411e-01 1.923600e-01 1 154343113 154343301 189 + 2.480 2.482 0.006
ENSG00000143515 E032 295.8269117 0.0002533144 1.060932e-02 3.023186e-02 1 154343453 154343568 116 + 2.479 2.460 -0.061
ENSG00000143515 E033 341.7239541 0.0001630359 1.730259e-02 4.560779e-02 1 154343893 154344039 147 + 2.537 2.527 -0.034
ENSG00000143515 E034 144.6316377 0.0002827169 3.153528e-01 4.581175e-01 1 154344040 154344057 18 + 2.160 2.166 0.021
ENSG00000143515 E035 12.6539721 0.0031389591 6.774688e-01 7.821827e-01 1 154344122 154344142 21 + 1.083 1.157 0.265
ENSG00000143515 E036 308.8912273 0.0001543623 1.994811e-02 5.137771e-02 1 154344143 154344254 112 + 2.494 2.483 -0.038
ENSG00000143515 E037 304.1917479 0.0008996179 3.959710e-01 5.397615e-01 1 154344395 154344500 106 + 2.473 2.488 0.052
ENSG00000143515 E038 378.8014261 0.0001873768 2.741943e-01 4.133237e-01 1 154344641 154344785 145 + 2.544 2.601 0.190
ENSG00000143515 E039 383.3020605 0.0001332767 1.226407e-02 3.416600e-02 1 154344971 154345154 184 + 2.535 2.618 0.276
ENSG00000143515 E040 377.9992377 0.0014976000 3.095588e-01 4.518765e-01 1 154345322 154345545 224 + 2.541 2.604 0.209
ENSG00000143515 E041 254.4162592 0.0002970356 6.950392e-01 7.957328e-01 1 154345800 154345883 84 + 2.388 2.415 0.090
ENSG00000143515 E042 485.2984756 0.0001586754 2.719130e-03 9.430143e-03 1 154346231 154346476 246 + 2.636 2.722 0.288
ENSG00000143515 E043 0.4481018 0.1528347571 6.356459e-01 7.498821e-01 1 154346615 154346619 5 + 0.120 0.219 1.045
ENSG00000143515 E044 394.8880846 0.0014642757 2.169357e-03 7.748008e-03 1 154346620 154346758 139 + 2.531 2.646 0.383
ENSG00000143515 E045 327.5785081 0.0014393716 9.836743e-05 5.142790e-04 1 154348408 154348538 131 + 2.434 2.574 0.469
ENSG00000143515 E046 1939.4972354 0.0043254246 7.775843e-12 1.649065e-10 1 154348840 154351304 2465 + 3.152 3.378 0.751