ENSG00000143514

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000343537 ENSG00000143514 HEK293_OSMI2_2hA HEK293_TMG_2hB TP53BP2 protein_coding protein_coding 19.57506 16.10576 20.06848 0.1427289 0.4950051 0.3171782 15.1005629 11.217034 17.0806144 1.2944145 0.3159175 0.6062274 0.76325833 0.69636667 0.85230000 0.15593333 2.635129e-01 4.274466e-05 FALSE TRUE
ENST00000391878 ENSG00000143514 HEK293_OSMI2_2hA HEK293_TMG_2hB TP53BP2 protein_coding protein_coding 19.57506 16.10576 20.06848 0.1427289 0.4950051 0.3171782 1.1584262 0.836016 1.4311817 0.5765770 0.2387059 0.7684953 0.05865417 0.05246667 0.07103333 0.01856667 6.320562e-01 4.274466e-05 FALSE TRUE
ENST00000483398 ENSG00000143514 HEK293_OSMI2_2hA HEK293_TMG_2hB TP53BP2 protein_coding nonsense_mediated_decay 19.57506 16.10576 20.06848 0.1427289 0.4950051 0.3171782 1.9853230 2.744118 0.6065779 0.4920822 0.1651356 -2.1592352 0.10755833 0.17043333 0.02986667 -0.14056667 4.274466e-05 4.274466e-05 FALSE TRUE
MSTRG.3110.1 ENSG00000143514 HEK293_OSMI2_2hA HEK293_TMG_2hB TP53BP2 protein_coding   19.57506 16.10576 20.06848 0.1427289 0.4950051 0.3171782 0.3439054 0.946644 0.0000000 0.8529027 0.0000000 -6.5799103 0.02000000 0.05810000 0.00000000 -0.05810000 2.229335e-01 4.274466e-05 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000143514 E001 1.3713738 0.2025133090 3.804687e-01 5.247464e-01 1 223779868 223779892 25 - 0.271 0.493 1.284
ENSG00000143514 E002 1.2263029 0.1223177576 8.162143e-01 8.837160e-01 1 223779893 223779898 6 - 0.334 0.388 0.317
ENSG00000143514 E003 162.6955074 0.0120362856 3.043862e-07 2.812915e-06 1 223779899 223780369 471 - 2.027 2.353 1.089
ENSG00000143514 E004 295.2821676 0.0033084460 9.669896e-16 3.658743e-14 1 223780370 223780644 275 - 2.310 2.594 0.949
ENSG00000143514 E005 209.6556864 0.0012286493 1.262377e-13 3.536729e-12 1 223780645 223780788 144 - 2.194 2.423 0.765
ENSG00000143514 E006 168.6634066 0.0005887304 4.393664e-11 8.229030e-10 1 223780789 223780894 106 - 2.117 2.322 0.684
ENSG00000143514 E007 227.8083456 0.0024821042 1.867007e-06 1.462291e-05 1 223784115 223784314 200 - 2.269 2.439 0.569
ENSG00000143514 E008 202.0735586 0.0037307007 3.087180e-04 1.415056e-03 1 223789008 223789174 167 - 2.229 2.380 0.503
ENSG00000143514 E009 157.8340725 0.0025735394 5.301823e-03 1.675253e-02 1 223792389 223792522 134 - 2.142 2.258 0.386
ENSG00000143514 E010 159.1172500 0.0003067273 5.746937e-02 1.222960e-01 1 223793303 223793440 138 - 2.171 2.238 0.224
ENSG00000143514 E011 85.5390004 0.0003988278 1.262291e-01 2.289059e-01 1 223795815 223795837 23 - 1.904 1.975 0.241
ENSG00000143514 E012 81.4128855 0.0003162945 2.491590e-01 3.849246e-01 1 223795838 223795862 25 - 1.886 1.944 0.195
ENSG00000143514 E013 221.3236493 0.0001841981 1.032476e-05 6.842252e-05 1 223795863 223796123 261 - 2.284 2.403 0.395
ENSG00000143514 E014 117.6863605 0.0002486391 7.606738e-02 1.532193e-01 1 223796124 223796173 50 - 2.037 2.108 0.237
ENSG00000143514 E015 265.7756363 0.0001835008 4.648758e-01 6.045562e-01 1 223796174 223796590 417 - 2.427 2.424 -0.010
ENSG00000143514 E016 256.3490105 0.0009387018 1.769269e-04 8.650770e-04 1 223798215 223798677 463 - 2.448 2.364 -0.279
ENSG00000143514 E017 74.5855964 0.0003696265 2.511286e-04 1.180067e-03 1 223799899 223799916 18 - 1.938 1.799 -0.468
ENSG00000143514 E018 128.9224960 0.0004000531 1.023819e-03 4.040905e-03 1 223799917 223800047 131 - 2.155 2.062 -0.308
ENSG00000143514 E019 103.1519896 0.0026753282 7.059218e-02 1.443246e-01 1 223800700 223800775 76 - 2.044 1.981 -0.213
ENSG00000143514 E020 84.0923543 0.0051030111 6.352802e-01 7.496227e-01 1 223800776 223800810 35 - 1.931 1.921 -0.033
ENSG00000143514 E021 0.2998086 0.0297110017 8.806045e-01   1 223801939 223802115 177 - 0.110 0.135 0.342
ENSG00000143514 E022 133.3255513 0.0048443553 7.542659e-02 1.522021e-01 1 223802116 223802300 185 - 2.156 2.089 -0.221
ENSG00000143514 E023 64.7232686 0.0003878243 3.247450e-02 7.676333e-02 1 223802301 223802344 44 - 1.852 1.771 -0.275
ENSG00000143514 E024 0.0000000       1 223802345 223802428 84 -      
ENSG00000143514 E025 0.0000000       1 223802429 223802669 241 -      
ENSG00000143514 E026 0.0000000       1 223802670 223802730 61 -      
ENSG00000143514 E027 140.5460350 0.0024836962 1.031906e-04 5.368139e-04 1 223802731 223802895 165 - 2.208 2.074 -0.450
ENSG00000143514 E028 4.1748548 0.0037219304 2.990872e-01 4.405692e-01 1 223802896 223803223 328 - 0.772 0.634 -0.572
ENSG00000143514 E029 153.9310522 0.0076908968 2.065194e-03 7.426677e-03 1 223803271 223803452 182 - 2.251 2.111 -0.467
ENSG00000143514 E030 79.3575613 0.0174666500 9.043863e-03 2.638698e-02 1 223804174 223804207 34 - 1.981 1.807 -0.587
ENSG00000143514 E031 74.4867703 0.0114513922 3.518070e-03 1.177894e-02 1 223804208 223804234 27 - 1.954 1.778 -0.593
ENSG00000143514 E032 131.8019910 0.0063649915 3.735529e-05 2.168883e-04 1 223804235 223804348 114 - 2.203 2.014 -0.634
ENSG00000143514 E033 104.9959850 0.0049958002 6.504245e-05 3.561094e-04 1 223806846 223806910 65 - 2.101 1.921 -0.606
ENSG00000143514 E034 80.8480730 0.0003122858 1.588101e-07 1.551790e-06 1 223806911 223806947 37 - 1.996 1.799 -0.663
ENSG00000143514 E035 79.9531448 0.0003095856 4.396030e-09 5.786650e-08 1 223810431 223810463 33 - 2.003 1.779 -0.754
ENSG00000143514 E036 90.4880788 0.0003182166 1.027976e-08 1.264181e-07 1 223810464 223810513 50 - 2.048 1.843 -0.689
ENSG00000143514 E037 0.1482932 0.0418049640 4.013963e-01   1 223814176 223814239 64 - 0.000 0.135 10.536
ENSG00000143514 E038 106.8481426 0.0002511717 3.311454e-16 1.334493e-14 1 223814240 223814353 114 - 2.145 1.869 -0.925
ENSG00000143514 E039 8.5099180 0.1145859473 3.566414e-01 5.010281e-01 1 223818206 223818337 132 - 1.023 0.899 -0.464
ENSG00000143514 E040 0.4439371 0.0215555088 1.212600e-01 2.217193e-01 1 223821069 223821219 151 - 0.271 0.000 -11.710
ENSG00000143514 E041 94.7050424 0.0003184076 1.550057e-13 4.288794e-12 1 223821220 223821367 148 - 2.090 1.826 -0.888
ENSG00000143514 E042 0.0000000       1 223825885 223826069 185 -      
ENSG00000143514 E043 62.0497324 0.0012621826 2.778616e-12 6.343704e-11 1 223845654 223846022 369 - 1.928 1.597 -1.119
ENSG00000143514 E044 0.4418608 0.0262527850 1.224698e-01 2.234698e-01 1 223846023 223846912 890 - 0.271 0.000 -11.710