ENSG00000143486

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000271764 ENSG00000143486 HEK293_OSMI2_2hA HEK293_TMG_2hB EIF2D protein_coding protein_coding 55.95252 63.62996 50.08289 8.005171 1.169271 -0.3453272 45.80822 57.31325 39.54848 7.421623 0.6223986 -0.5351337 0.8158917 0.8999 0.7900333 -0.1098667 3.62115e-09 3.62115e-09 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000143486 E001 0.3332198 0.0266370409 7.108753e-01   1 206571292 206571493 202 - 0.153 0.097 -0.744
ENSG00000143486 E002 0.2214452 0.0548268456 7.445750e-01   1 206572595 206572699 105 - 0.000 0.098 8.364
ENSG00000143486 E003 0.1482932 0.0411597534 7.492746e-01   1 206580692 206580768 77 - 0.000 0.097 8.355
ENSG00000143486 E004 0.7415677 0.0673731005 8.698940e-01 9.203094e-01 1 206580935 206581162 228 - 0.265 0.242 -0.176
ENSG00000143486 E005 392.7299871 0.0009160579 3.818081e-03 1.263161e-02 1 206591642 206591845 204 - 2.532 2.597 0.216
ENSG00000143486 E006 627.1064665 0.0001504320 9.630840e-10 1.432580e-08 1 206593619 206593793 175 - 2.716 2.809 0.310
ENSG00000143486 E007 98.7552482 0.0091038087 3.240359e-04 1.477014e-03 1 206594040 206595709 1670 - 2.110 1.888 -0.743
ENSG00000143486 E008 6.9791368 0.0028733143 5.525939e-01 6.818652e-01 1 206595710 206595717 8 - 0.934 0.862 -0.272
ENSG00000143486 E009 516.3339803 0.0001876750 1.165868e-07 1.170049e-06 1 206595718 206595830 113 - 2.634 2.724 0.298
ENSG00000143486 E010 268.3369630 0.0025749589 1.025638e-02 2.939725e-02 1 206595831 206595838 8 - 2.354 2.432 0.261
ENSG00000143486 E011 452.1748907 0.0001838651 5.668671e-04 2.409753e-03 1 206597100 206597195 96 - 2.596 2.657 0.203
ENSG00000143486 E012 463.7009994 0.0001645357 1.373426e-01 2.443685e-01 1 206599003 206599092 90 - 2.632 2.657 0.083
ENSG00000143486 E013 424.9656732 0.0001394474 3.550018e-04 1.599648e-03 1 206599463 206599612 150 - 2.567 2.632 0.215
ENSG00000143486 E014 8.8508249 0.0022477819 3.529891e-02 8.222495e-02 1 206599613 206599732 120 - 1.107 0.877 -0.851
ENSG00000143486 E015 389.7029929 0.0009334661 6.699680e-02 1.383791e-01 1 206599733 206599836 104 - 2.546 2.584 0.126
ENSG00000143486 E016 340.8224890 0.0001610958 5.231862e-02 1.132871e-01 1 206600263 206600308 46 - 2.542 2.502 -0.132
ENSG00000143486 E017 57.7536391 0.0098324796 2.638779e-02 6.472485e-02 1 206600309 206602335 2027 - 1.843 1.685 -0.536
ENSG00000143486 E018 245.1307490 0.0001944836 1.024831e-01 1.942911e-01 1 206602336 206602341 6 - 2.399 2.360 -0.131
ENSG00000143486 E019 441.5248609 0.0001694444 9.541630e-03 2.763965e-02 1 206602342 206602453 112 - 2.659 2.613 -0.155
ENSG00000143486 E020 14.8015209 0.0011876064 9.267229e-10 1.382183e-08 1 206602454 206602950 497 - 1.453 0.919 -1.909
ENSG00000143486 E021 444.8788158 0.0003373304 2.861900e-01 4.265931e-01 1 206602951 206603082 132 - 2.646 2.625 -0.070
ENSG00000143486 E022 404.7554809 0.0001588508 2.155077e-01 3.450305e-01 1 206603083 206603204 122 - 2.606 2.582 -0.080
ENSG00000143486 E023 14.3651816 0.0278776648 7.437222e-03 2.235556e-02 1 206603205 206603501 297 - 1.353 1.031 -1.146
ENSG00000143486 E024 2.7238964 0.0056954374 7.907105e-02 1.580262e-01 1 206604609 206604729 121 - 0.717 0.441 -1.259
ENSG00000143486 E025 11.7703337 0.0207120508 2.635603e-03 9.176358e-03 1 206604733 206605399 667 - 1.281 0.921 -1.303
ENSG00000143486 E026 384.0556854 0.0001431024 9.832640e-02 1.879757e-01 1 206605400 206605507 108 - 2.588 2.556 -0.107
ENSG00000143486 E027 228.5095758 0.0007847877 3.074456e-02 7.340960e-02 1 206608236 206608279 44 - 2.381 2.320 -0.206
ENSG00000143486 E028 236.0230171 0.0027712130 5.017067e-01 6.378468e-01 1 206608280 206608326 47 - 2.373 2.344 -0.096
ENSG00000143486 E029 355.0216072 0.0002444181 8.997490e-03 2.627312e-02 1 206609376 206609459 84 - 2.568 2.514 -0.180
ENSG00000143486 E030 1.9291488 0.1588449650 2.299179e-01 3.623551e-01 1 206611148 206611183 36 - 0.597 0.388 -1.029
ENSG00000143486 E031 501.6661770 0.0007389154 6.440560e-04 2.694547e-03 1 206611184 206611374 191 - 2.728 2.655 -0.244
ENSG00000143486 E032 277.2330976 0.0011279908 1.181390e-04 6.052921e-04 1 206612287 206612539 253 - 2.492 2.385 -0.356