ENSG00000143476

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000366991 ENSG00000143476 HEK293_OSMI2_2hA HEK293_TMG_2hB DTL protein_coding protein_coding 29.52845 14.87672 31.37573 3.111239 0.5485869 1.076083 18.844172 12.766315 21.000413 1.299196 0.7496236 0.71763271 0.6720667 0.8932333 0.6701333 -0.2231000 2.826596e-01 3.416587e-45 FALSE TRUE
ENST00000496442 ENSG00000143476 HEK293_OSMI2_2hA HEK293_TMG_2hB DTL protein_coding processed_transcript 29.52845 14.87672 31.37573 3.111239 0.5485869 1.076083 4.075830 1.802004 1.878501 1.802004 0.9404549 0.05965491 0.1342458 0.0855000 0.0589000 -0.0266000 6.822305e-01 3.416587e-45 FALSE FALSE
MSTRG.3012.2 ENSG00000143476 HEK293_OSMI2_2hA HEK293_TMG_2hB DTL protein_coding   29.52845 14.87672 31.37573 3.111239 0.5485869 1.076083 5.315716 0.000000 7.224160 0.000000 0.1888507 9.49868163 0.1421708 0.0000000 0.2305667 0.2305667 3.416587e-45 3.416587e-45 TRUE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000143476 E001 3.7665970 0.0044945953 3.739799e-02 8.622291e-02 1 212035553 212035747 195 + 0.755 0.448 -1.375
ENSG00000143476 E002 76.0086823 0.0003797906 2.489871e-01 3.847241e-01 1 212035748 212035845 98 + 1.882 1.873 -0.032
ENSG00000143476 E003 126.6578094 0.0002811033 6.049366e-03 1.874075e-02 1 212035846 212035942 97 + 2.115 2.063 -0.174
ENSG00000143476 E004 157.9619134 0.0002954545 6.237870e-06 4.344973e-05 1 212042993 212043118 126 + 2.226 2.129 -0.325
ENSG00000143476 E005 157.8812597 0.0012511804 3.016459e-06 2.255611e-05 1 212044660 212044758 99 + 2.235 2.114 -0.405
ENSG00000143476 E006 131.7453304 0.0002680827 7.384125e-06 5.055292e-05 1 212047151 212047212 62 + 2.152 2.044 -0.361
ENSG00000143476 E007 173.8004633 0.0014671783 6.042971e-06 4.223191e-05 1 212047297 212047417 121 + 2.273 2.158 -0.385
ENSG00000143476 E008 136.7756700 0.0062648255 4.817045e-03 1.542163e-02 1 212062884 212062949 66 + 2.168 2.059 -0.365
ENSG00000143476 E009 152.8207624 0.0033324587 2.040689e-03 7.352931e-03 1 212064917 212065029 113 + 2.210 2.116 -0.314
ENSG00000143476 E010 129.5919929 0.0002343358 1.586777e-01 2.733644e-01 1 212066812 212066885 74 + 2.111 2.105 -0.021
ENSG00000143476 E011 121.5230998 0.0002593638 2.031549e-01 3.301713e-01 1 212068224 212068306 83 + 2.083 2.080 -0.011
ENSG00000143476 E012 100.8189098 0.0003005943 5.058336e-01 6.415608e-01 1 212068307 212068327 21 + 1.995 2.009 0.049
ENSG00000143476 E013 158.2462834 0.0004320834 4.266869e-03 1.389005e-02 1 212068599 212068703 105 + 2.211 2.163 -0.161
ENSG00000143476 E014 0.8136755 0.0145807488 8.626028e-01 9.153965e-01 1 212072074 212072100 27 + 0.231 0.280 0.361
ENSG00000143476 E015 175.5766090 0.0023362194 8.160342e-03 2.418952e-02 1 212072101 212072213 113 + 2.259 2.196 -0.209
ENSG00000143476 E016 166.0924321 0.0002529559 4.540628e-02 1.009397e-01 1 212078173 212078262 90 + 2.221 2.203 -0.063
ENSG00000143476 E017 0.4418608 0.0258458121 2.208298e-01 3.514204e-01 1 212080369 212080614 246 + 0.231 0.000 -10.447
ENSG00000143476 E018 170.7684193 0.0002483944 6.287253e-01 7.442150e-01 1 212080615 212080750 136 + 2.207 2.259 0.173
ENSG00000143476 E019 338.2698081 0.0002890169 2.634045e-01 4.013028e-01 1 212100252 212100645 394 + 2.499 2.560 0.203
ENSG00000143476 E020 172.6248599 0.0002291391 8.718319e-01 9.215666e-01 1 212100646 212100700 55 + 2.217 2.260 0.145
ENSG00000143476 E021 394.6140771 0.0009987292 1.011348e-03 3.997764e-03 1 212100701 212101084 384 + 2.544 2.656 0.373
ENSG00000143476 E022 893.4907364 0.0047485176 2.134382e-08 2.475525e-07 1 212102842 212105013 2172 + 2.855 3.051 0.652
ENSG00000143476 E023 0.1472490 0.0441418990 8.734195e-01   1 212107355 212107400 46 + 0.091 0.000 -8.862