ENSG00000143458

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000368918 ENSG00000143458 HEK293_OSMI2_2hA HEK293_TMG_2hB GABPB2 protein_coding protein_coding 3.711009 2.89733 5.234277 0.3551685 0.2858252 0.8510491 1.4138979 1.00311945 1.9553572 0.1545492 0.14852061 0.9559873 0.38263333 0.36370000 0.37733333 0.01363333 0.95286938 0.03642304 FALSE  
ENST00000486076 ENSG00000143458 HEK293_OSMI2_2hA HEK293_TMG_2hB GABPB2 protein_coding processed_transcript 3.711009 2.89733 5.234277 0.3551685 0.2858252 0.8510491 0.2607280 0.21947230 0.3417612 0.0124870 0.10691214 0.6162762 0.07045833 0.07900000 0.06490000 -0.01410000 0.90929831 0.03642304 FALSE  
MSTRG.2198.1 ENSG00000143458 HEK293_OSMI2_2hA HEK293_TMG_2hB GABPB2 protein_coding   3.711009 2.89733 5.234277 0.3551685 0.2858252 0.8510491 0.4283129 0.60513517 0.3919651 0.3605742 0.39196507 -0.6138333 0.13890417 0.23010000 0.08380000 -0.14630000 0.52186659 0.03642304 FALSE  
MSTRG.2198.2 ENSG00000143458 HEK293_OSMI2_2hA HEK293_TMG_2hB GABPB2 protein_coding   3.711009 2.89733 5.234277 0.3551685 0.2858252 0.8510491 0.3146466 0.25322811 0.7672330 0.1370944 0.18010213 1.5620335 0.07698750 0.09386667 0.14366667 0.04980000 0.67857264 0.03642304 FALSE  
MSTRG.2198.3 ENSG00000143458 HEK293_OSMI2_2hA HEK293_TMG_2hB GABPB2 protein_coding   3.711009 2.89733 5.234277 0.3551685 0.2858252 0.8510491 0.5549329 0.72242035 0.2037237 0.7224204 0.20372371 -1.7769249 0.15327083 0.20280000 0.03773333 -0.16506667 0.92548292 0.03642304 FALSE  
MSTRG.2198.5 ENSG00000143458 HEK293_OSMI2_2hA HEK293_TMG_2hB GABPB2 protein_coding   3.711009 2.89733 5.234277 0.3551685 0.2858252 0.8510491 0.2441685 0.06990889 0.4979918 0.0200013 0.07259027 2.6683773 0.06224583 0.02376667 0.09683333 0.07306667 0.03642304 0.03642304 FALSE  
MSTRG.2198.9 ENSG00000143458 HEK293_OSMI2_2hA HEK293_TMG_2hB GABPB2 protein_coding   3.711009 2.89733 5.234277 0.3551685 0.2858252 0.8510491 0.2955368 0.00000000 0.8616637 0.0000000 0.43433047 6.4456997 0.06618750 0.00000000 0.15613333 0.15613333 0.24743935 0.03642304 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000143458 E001 1.099765 0.0129938977 2.348097e-02 5.874479e-02 1 151070517 151070553 37 + 0.099 0.487 3.009
ENSG00000143458 E002 1.244938 0.0248370872 7.836074e-02 1.568482e-01 1 151070554 151070564 11 + 0.180 0.487 2.012
ENSG00000143458 E003 1.244938 0.0248370872 7.836074e-02 1.568482e-01 1 151070565 151070577 13 + 0.180 0.487 2.012
ENSG00000143458 E004 4.760641 0.0033350380 8.245268e-01 8.894580e-01 1 151070578 151070760 183 + 0.750 0.775 0.100
ENSG00000143458 E005 4.065057 0.0328391523 4.966681e-01 6.333025e-01 1 151070761 151070776 16 + 0.750 0.633 -0.488
ENSG00000143458 E006 7.658180 0.0340373654 1.880378e-01 3.112501e-01 1 151070777 151070803 27 + 1.010 0.827 -0.693
ENSG00000143458 E007 25.961795 0.0008514544 3.086461e-01 4.509160e-01 1 151070804 151070934 131 + 1.458 1.382 -0.262
ENSG00000143458 E008 4.133138 0.0041293813 7.263956e-01 8.194328e-01 1 151073039 151073157 119 + 0.729 0.673 -0.232
ENSG00000143458 E009 1.960457 0.0982198107 7.863183e-01 8.631386e-01 1 151088158 151088189 32 + 0.447 0.487 0.204
ENSG00000143458 E010 32.281286 0.0006943455 6.175903e-01 7.353628e-01 1 151088190 151088297 108 + 1.513 1.537 0.082
ENSG00000143458 E011 2.652922 0.0058003293 4.652613e-01 6.049786e-01 1 151088470 151088517 48 + 0.611 0.486 -0.578
ENSG00000143458 E012 19.921094 0.0009482151 1.504966e-01 2.623595e-01 1 151090406 151090457 52 + 1.272 1.375 0.358
ENSG00000143458 E013 24.779764 0.0008719923 8.614568e-02 1.692283e-01 1 151090458 151090573 116 + 1.358 1.469 0.384
ENSG00000143458 E014 18.818451 0.0010715620 9.696238e-01 9.849982e-01 1 151093192 151093245 54 + 1.295 1.292 -0.013
ENSG00000143458 E015 29.669379 0.0007123511 9.331539e-01 9.618656e-01 1 151093246 151093386 141 + 1.488 1.487 -0.003
ENSG00000143458 E016 32.110250 0.0006895255 1.649477e-01 2.816303e-01 1 151097852 151098002 151 + 1.555 1.463 -0.315
ENSG00000143458 E017 16.649513 0.0348074028 8.988568e-01 9.394867e-01 1 151103562 151103675 114 + 1.254 1.244 -0.034
ENSG00000143458 E018 42.492014 0.0041035566 1.167110e-01 2.152653e-01 1 151107037 151107222 186 + 1.676 1.576 -0.343
ENSG00000143458 E019 32.155940 0.0006427679 2.023799e-01 3.292000e-01 1 151117392 151117516 125 + 1.549 1.463 -0.293
ENSG00000143458 E020 40.916295 0.0051252634 2.243466e-01 3.556248e-01 1 151117957 151118139 183 + 1.649 1.564 -0.292
ENSG00000143458 E021 266.322034 0.0167531932 3.686663e-02 8.521250e-02 1 151118140 151124186 6047 + 2.464 2.358 -0.353
ENSG00000143458 E022 116.647594 0.0111433761 7.635553e-08 7.932512e-07 1 151124187 151125542 1356 + 1.924 2.209 0.956
ENSG00000143458 E023 1.483225 0.0089071248 2.227781e-01 3.536916e-01 1 151128901 151129029 129 + 0.485 0.261 -1.316