ENSG00000143457

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000271732 ENSG00000143457 HEK293_OSMI2_2hA HEK293_TMG_2hB GOLPH3L protein_coding protein_coding 16.95609 11.77974 23.72727 1.65253 0.6778715 1.009623 13.6375738 11.2782258 17.087615 1.5642615 0.5489541 0.5989763 0.83971250 0.9578 0.72023333 -0.23756667 1.111521e-24 1.111521e-24 FALSE TRUE
ENST00000427665 ENSG00000143457 HEK293_OSMI2_2hA HEK293_TMG_2hB GOLPH3L protein_coding protein_coding 16.95609 11.77974 23.72727 1.65253 0.6778715 1.009623 0.8563342 0.3185439 2.142828 0.1629450 0.1420760 2.7120753 0.04845000 0.0262 0.09023333 0.06403333 1.928617e-01 1.111521e-24 FALSE FALSE
MSTRG.2176.3 ENSG00000143457 HEK293_OSMI2_2hA HEK293_TMG_2hB GOLPH3L protein_coding   16.95609 11.77974 23.72727 1.65253 0.6778715 1.009623 2.1759445 0.1829669 4.160242 0.1187242 0.2635494 4.4337060 0.09219167 0.0160 0.17610000 0.16010000 1.070111e-03 1.111521e-24 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000143457 E001 794.8034559 0.0013423254 2.565318e-11 5.000430e-10 1 150646230 150648436 2207 - 2.851 2.958 0.357
ENSG00000143457 E002 262.8434136 0.0002056285 3.405014e-02 7.985710e-02 1 150648437 150648748 312 - 2.431 2.413 -0.060
ENSG00000143457 E003 1.6712722 0.0106332196 3.389159e-01 4.828217e-01 1 150649164 150649227 64 - 0.494 0.342 -0.818
ENSG00000143457 E004 131.5415535 0.0057605264 8.637634e-02 1.696041e-01 1 150661814 150661928 115 - 2.148 2.096 -0.173
ENSG00000143457 E005 122.6031814 0.0002706697 4.619449e-02 1.023953e-01 1 150663632 150663763 132 - 2.111 2.076 -0.120
ENSG00000143457 E006 4.2812926 0.0409003970 2.879587e-01 4.284951e-01 1 150694108 150694173 66 - 0.780 0.624 -0.646
ENSG00000143457 E007 0.7331366 0.0177970400 3.209317e-01 4.641045e-01 1 150694512 150694655 144 - 0.313 0.146 -1.402
ENSG00000143457 E008 177.0944851 0.0002355062 5.261920e-06 3.725864e-05 1 150694656 150694850 195 - 2.290 2.197 -0.311
ENSG00000143457 E009 108.6822376 0.0035666566 9.574830e-05 5.020545e-04 1 150696992 150697162 171 - 2.100 1.947 -0.512