ENSG00000143321

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000357325 ENSG00000143321 HEK293_OSMI2_2hA HEK293_TMG_2hB HDGF protein_coding protein_coding 528.7396 966.4529 295.3677 40.20144 8.186079 -1.710153 415.78902 804.4005 210.2674 21.74353 4.860227 -1.9356384 0.7555750 0.8334000 0.7121000 -0.1213 6.532193e-06 1.072806e-09 FALSE TRUE
ENST00000482651 ENSG00000143321 HEK293_OSMI2_2hA HEK293_TMG_2hB HDGF protein_coding processed_transcript 528.7396 966.4529 295.3677 40.20144 8.186079 -1.710153 89.58317 129.0185 70.4428 15.85094 3.083433 -0.8729606 0.1966417 0.1325333 0.2383333 0.1058 1.510698e-05 1.072806e-09 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000143321 E001 48.744374 5.835664e-03 8.580069e-11 1.531871e-09 1 156742109 156742109 1 - 0.981 1.639 2.311
ENSG00000143321 E002 7541.348142 1.860567e-03 5.986313e-16 2.328725e-14 1 156742110 156742525 416 - 3.605 3.775 0.567
ENSG00000143321 E003 10007.046112 2.006964e-04 1.500493e-29 2.421328e-27 1 156742526 156743012 487 - 3.797 3.887 0.301
ENSG00000143321 E004 3676.307798 1.536244e-04 9.390931e-02 1.812450e-01 1 156743013 156743155 143 - 3.422 3.441 0.063
ENSG00000143321 E005 2702.531330 7.048535e-05 9.897907e-01 9.976955e-01 1 156743156 156743170 15 - 3.302 3.305 0.008
ENSG00000143321 E006 6466.671963 3.695369e-05 6.328281e-01 7.476187e-01 1 156743171 156743306 136 - 3.678 3.684 0.018
ENSG00000143321 E007 3982.644593 1.921063e-04 3.923508e-02 8.961692e-02 1 156743307 156743344 38 - 3.453 3.477 0.078
ENSG00000143321 E008 5517.937552 1.236833e-04 3.357120e-03 1.131283e-02 1 156743345 156743455 111 - 3.592 3.618 0.088
ENSG00000143321 E009 93.049904 1.658896e-03 1.773917e-05 1.113050e-04 1 156743456 156743651 196 - 2.002 1.803 -0.669
ENSG00000143321 E010 3699.114360 7.127646e-05 1.960608e-03 7.102481e-03 1 156743652 156743708 57 - 3.416 3.445 0.095
ENSG00000143321 E011 4229.955154 5.634638e-04 4.676706e-01 6.071691e-01 1 156743709 156743802 94 - 3.489 3.502 0.043
ENSG00000143321 E012 3728.520772 5.788649e-05 7.830969e-01 8.607646e-01 1 156743803 156743840 38 - 3.439 3.444 0.015
ENSG00000143321 E013 3818.288502 4.319375e-05 8.200660e-02 1.626881e-01 1 156743841 156743878 38 - 3.463 3.452 -0.037
ENSG00000143321 E014 7737.412515 3.072119e-05 8.471346e-17 3.680897e-15 1 156744163 156744348 186 - 3.795 3.753 -0.141
ENSG00000143321 E015 18.691618 9.635666e-04 1.372276e-01 2.442207e-01 1 156744349 156744369 21 - 1.037 1.198 0.581
ENSG00000143321 E016 33.652789 9.845392e-03 1.820411e-06 1.429045e-05 1 156744501 156744570 70 - 1.697 1.319 -1.299
ENSG00000143321 E017 5619.045875 3.345221e-04 2.045516e-24 2.111808e-22 1 156745008 156745146 139 - 3.703 3.604 -0.329
ENSG00000143321 E018 38.122526 5.544049e-04 1.688079e-07 1.641354e-06 1 156745147 156745296 150 - 1.719 1.392 -1.121
ENSG00000143321 E019 3240.832398 1.276427e-03 2.467778e-16 1.012591e-14 1 156745297 156745373 77 - 3.502 3.355 -0.488
ENSG00000143321 E020 5.861458 2.845711e-03 1.738817e-01 2.932370e-01 1 156747435 156747471 37 - 0.880 0.687 -0.772
ENSG00000143321 E021 2046.119183 3.396502e-03 9.314555e-22 7.211338e-20 1 156751343 156751675 333 - 3.401 3.124 -0.923
ENSG00000143321 E022 12.604570 5.120915e-03 1.081605e-01 2.028157e-01 1 156752038 156752228 191 - 1.152 0.972 -0.653
ENSG00000143321 E023 73.908245 3.747780e-04 2.286067e-02 5.744946e-02 1 156752229 156752448 220 - 1.838 1.725 -0.381
ENSG00000143321 E024 6.745845 2.257231e-02 2.854505e-01 4.257722e-01 1 156755537 156755664 128 - 0.916 0.762 -0.601
ENSG00000143321 E025 1.772534 8.349003e-03 4.357123e-01 5.779021e-01 1 156758983 156759219 237 - 0.474 0.328 -0.812
ENSG00000143321 E026 2.647546 1.606816e-02 2.786097e-01 4.182683e-01 1 156766790 156766925 136 - 0.633 0.442 -0.900
ENSG00000143321 E027 4.388732 3.713946e-03 1.781056e-01 2.987646e-01 1 156767339 156767476 138 - 0.797 0.590 -0.866