ENSG00000143294

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000271526 ENSG00000143294 HEK293_OSMI2_2hA HEK293_TMG_2hB PRCC protein_coding protein_coding 126.0305 212.12 92.05895 1.356654 0.6697143 -1.204162 82.259357 130.3326650 59.9719120 9.6207366 1.7760453 -1.119710 0.66078333 0.614800000 0.65136667 0.03656667 0.8117199669 0.0001183542 FALSE TRUE
ENST00000473387 ENSG00000143294 HEK293_OSMI2_2hA HEK293_TMG_2hB PRCC protein_coding processed_transcript 126.0305 212.12 92.05895 1.356654 0.6697143 -1.204162 9.301460 0.2597449 19.6438607 0.2597449 0.1388984 6.187073 0.10354167 0.001233333 0.21340000 0.21216667 0.0001183542 0.0001183542 FALSE FALSE
ENST00000526188 ENSG00000143294 HEK293_OSMI2_2hA HEK293_TMG_2hB PRCC protein_coding protein_coding 126.0305 212.12 92.05895 1.356654 0.6697143 -1.204162 9.049034 20.5923435 5.4845125 3.1579101 0.7213358 -1.906745 0.06208333 0.097033333 0.05956667 -0.03746667 0.1942461435 0.0001183542 FALSE TRUE
MSTRG.2381.2 ENSG00000143294 HEK293_OSMI2_2hA HEK293_TMG_2hB PRCC protein_coding   126.0305 212.12 92.05895 1.356654 0.6697143 -1.204162 17.489032 50.3134557 0.9095317 7.8690948 0.9095317 -5.774188 0.10463333 0.236900000 0.00990000 -0.22700000 0.0136278884 0.0001183542 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000143294 E001 0.7019140 1.755447e-02 3.076013e-01 4.498232e-01 1 156750610 156750731 122 + 0.319 0.143 -1.477
ENSG00000143294 E002 0.1482932 4.118157e-02 1.000000e+00   1 156760722 156760777 56 + 0.001 0.077 6.658
ENSG00000143294 E003 3.2272029 6.300348e-02 6.129586e-01 7.317796e-01 1 156767339 156767347 9 + 0.629 0.549 -0.359
ENSG00000143294 E004 6.6788829 9.103489e-02 8.743349e-01 9.232080e-01 1 156767348 156767416 69 + 0.770 0.836 0.257
ENSG00000143294 E005 36.0720354 2.901511e-02 2.669604e-01 4.052341e-01 1 156767417 156767534 118 + 1.566 1.468 -0.336
ENSG00000143294 E006 101.9845446 1.579121e-02 7.133174e-06 4.901376e-05 1 156767535 156767552 18 + 2.146 1.845 -1.012
ENSG00000143294 E007 394.8863772 5.957467e-03 1.542965e-01 2.674898e-01 1 156767553 156767583 31 + 2.554 2.510 -0.148
ENSG00000143294 E008 1547.4522514 3.476660e-03 8.703880e-02 1.706539e-01 1 156767584 156768108 525 + 3.139 3.106 -0.109
ENSG00000143294 E009 725.0000753 8.063530e-04 1.255411e-05 8.155330e-05 1 156768109 156768239 131 + 2.838 2.766 -0.242
ENSG00000143294 E010 516.5596607 3.049660e-04 1.210018e-03 4.674626e-03 1 156782282 156782329 48 + 2.673 2.624 -0.164
ENSG00000143294 E011 1553.1759542 7.058239e-05 3.861691e-13 1.010338e-11 1 156786608 156786874 267 + 3.161 3.100 -0.203
ENSG00000143294 E012 646.6116964 3.776540e-04 1.369341e-04 6.899770e-04 1 156786875 156786890 16 + 2.778 2.723 -0.181
ENSG00000143294 E013 1828.3365410 7.670625e-05 2.470390e-04 1.162955e-03 1 156786891 156787174 284 + 3.202 3.180 -0.074
ENSG00000143294 E014 24.2927781 1.766292e-03 1.540424e-05 9.804427e-05 1 156791067 156791162 96 + 1.547 1.240 -1.064
ENSG00000143294 E015 18.9288889 2.346634e-02 2.626503e-02 6.447968e-02 1 156791376 156791696 321 + 1.396 1.176 -0.773
ENSG00000143294 E016 595.8577688 3.856480e-04 3.264106e-03 1.104438e-02 1 156791697 156791729 33 + 2.643 2.713 0.233
ENSG00000143294 E017 793.9968467 1.189195e-04 1.135788e-03 4.423850e-03 1 156791730 156791792 63 + 2.775 2.838 0.210
ENSG00000143294 E018 1398.8530506 4.925254e-04 5.904601e-04 2.498791e-03 1 156794665 156794808 144 + 3.020 3.084 0.215
ENSG00000143294 E019 26.5854511 1.158498e-01 2.562782e-01 3.932317e-01 1 156795812 156797275 1464 + 1.441 1.335 -0.369
ENSG00000143294 E020 669.3330785 1.573086e-03 2.495090e-02 6.177063e-02 1 156797276 156797278 3 + 2.696 2.766 0.235
ENSG00000143294 E021 1126.2611610 1.800620e-03 1.107343e-03 4.327649e-03 1 156797279 156797341 63 + 2.905 2.996 0.302
ENSG00000143294 E022 2043.7082444 1.882703e-03 5.269454e-09 6.846460e-08 1 156800374 156800815 442 + 3.126 3.263 0.455