ENSG00000143228

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000271452 ENSG00000143228 HEK293_OSMI2_2hA HEK293_TMG_2hB NUF2 protein_coding protein_coding 23.99341 6.923094 38.43655 0.7802312 0.9167685 2.471283 9.2579765 4.0015276 11.7612585 0.5085708 2.0264574 1.5530449 0.4524458 0.57700000 0.3086333 -0.26836667 1.245674e-02 2.792494e-19 FALSE TRUE
ENST00000367900 ENSG00000143228 HEK293_OSMI2_2hA HEK293_TMG_2hB NUF2 protein_coding protein_coding 23.99341 6.923094 38.43655 0.7802312 0.9167685 2.471283 7.8273792 1.9001013 12.9403425 0.7465504 1.8791325 2.7612692 0.3007458 0.25866667 0.3347667 0.07610000 7.384607e-01 2.792494e-19 FALSE TRUE
MSTRG.2496.2 ENSG00000143228 HEK293_OSMI2_2hA HEK293_TMG_2hB NUF2 protein_coding   23.99341 6.923094 38.43655 0.7802312 0.9167685 2.471283 0.8200987 0.4189286 0.6489653 0.4189286 0.1848615 0.6194649 0.0403375 0.07356667 0.0171000 -0.05646667 7.607536e-01 2.792494e-19 FALSE TRUE
MSTRG.2496.9 ENSG00000143228 HEK293_OSMI2_2hA HEK293_TMG_2hB NUF2 protein_coding   23.99341 6.923094 38.43655 0.7802312 0.9167685 2.471283 3.0403945 0.0000000 8.5084305 0.0000000 1.2057324 9.7344438 0.0757375 0.00000000 0.2202000 0.22020000 2.792494e-19 2.792494e-19 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000143228 E001 0.0000000       1 163266576 163266674 99 +      
ENSG00000143228 E002 0.0000000       1 163267270 163267344 75 +      
ENSG00000143228 E003 0.1515154 0.0429178317 1.000000e+00   1 163321889 163321889 1 + 0.060 0.001 -5.941
ENSG00000143228 E004 0.3332198 0.0330726617 2.559512e-01   1 163321890 163321899 10 + 0.060 0.234 2.272
ENSG00000143228 E005 0.7739347 0.0150380147 2.368108e-01 3.704908e-01 1 163321900 163321934 35 + 0.160 0.385 1.686
ENSG00000143228 E006 3.8688092 0.0040581702 1.545630e-01 2.678342e-01 1 163321935 163321953 19 + 0.658 0.386 -1.313
ENSG00000143228 E007 8.5587957 0.0023266653 6.558435e-02 1.360171e-01 1 163321954 163321963 10 + 0.940 0.660 -1.107
ENSG00000143228 E008 27.3266454 0.0028543809 9.555849e-06 6.381356e-05 1 163321964 163321985 22 + 1.423 0.948 -1.695
ENSG00000143228 E009 56.3602287 0.0065210253 3.512747e-03 1.176299e-02 1 163321986 163322045 60 + 1.700 1.481 -0.747
ENSG00000143228 E010 62.5568409 0.0009786223 3.079296e-02 7.351221e-02 1 163322046 163322066 21 + 1.730 1.606 -0.423
ENSG00000143228 E011 80.1061571 0.0060336301 1.892333e-01 3.127726e-01 1 163322067 163322212 146 + 1.827 1.750 -0.261
ENSG00000143228 E012 3.8330942 0.0808155808 2.195713e-01 3.498751e-01 1 163322867 163323095 229 + 0.658 0.383 -1.324
ENSG00000143228 E013 85.1717066 0.0003280986 2.226565e-02 5.622446e-02 1 163326032 163326074 43 + 1.858 1.749 -0.368
ENSG00000143228 E014 132.7782492 0.0002716098 6.352715e-05 3.487100e-04 1 163326075 163326174 100 + 2.057 1.897 -0.535
ENSG00000143228 E015 118.9374689 0.0003008861 8.504588e-04 3.439508e-03 1 163327488 163327562 75 + 2.007 1.869 -0.465
ENSG00000143228 E016 0.0000000       1 163327898 163327930 33 +      
ENSG00000143228 E017 106.5161637 0.0003548316 1.244687e-02 3.461013e-02 1 163328228 163328304 77 + 1.954 1.847 -0.360
ENSG00000143228 E018 5.9665110 0.0081440280 5.673996e-01 6.942206e-01 1 163328305 163328457 153 + 0.736 0.828 0.366
ENSG00000143228 E019 5.9729544 0.0053676077 5.685363e-01 6.951918e-01 1 163328458 163328530 73 + 0.736 0.828 0.365
ENSG00000143228 E020 2.5550956 0.0067674550 1.620395e-01 2.777687e-01 1 163328531 163328561 31 + 0.528 0.234 -1.728
ENSG00000143228 E021 120.3640192 0.0003124167 1.311488e-01 2.357525e-01 1 163328846 163328907 62 + 1.998 1.942 -0.189
ENSG00000143228 E022 137.7319823 0.0002372084 1.159257e-01 2.141317e-01 1 163336751 163336848 98 + 2.056 2.002 -0.181
ENSG00000143228 E023 0.8136755 0.0144163423 2.376815e-01 3.714665e-01 1 163338015 163338019 5 + 0.160 0.386 1.687
ENSG00000143228 E024 130.3459205 0.0002285134 2.868208e-01 4.272676e-01 1 163338020 163338093 74 + 2.028 1.992 -0.118
ENSG00000143228 E025 2.5237607 0.0058069830 1.607342e-01 2.760808e-01 1 163339281 163339380 100 + 0.528 0.234 -1.729
ENSG00000143228 E026 136.8634727 0.0011975995 9.914904e-01 9.987605e-01 1 163339381 163339477 97 + 2.040 2.046 0.021
ENSG00000143228 E027 1.5919784 0.0097133454 8.295735e-01 8.930017e-01 1 163340255 163340363 109 + 0.339 0.386 0.273
ENSG00000143228 E028 67.7477705 0.0015477832 4.967422e-01 6.333543e-01 1 163340364 163340367 4 + 1.747 1.714 -0.108
ENSG00000143228 E029 113.2361747 0.0020084123 6.029196e-01 7.237946e-01 1 163340368 163340426 59 + 1.952 1.984 0.107
ENSG00000143228 E030 145.0596112 0.0037721645 1.608491e-01 2.762284e-01 1 163343733 163343870 138 + 2.050 2.123 0.244
ENSG00000143228 E031 167.0796651 0.0027702901 3.700415e-02 8.548120e-02 1 163345678 163345818 141 + 2.105 2.199 0.316
ENSG00000143228 E032 1.3274553 0.2331154983 2.044253e-01 3.316926e-01 1 163345819 163346240 422 + 0.240 0.573 1.893
ENSG00000143228 E033 200.9187449 0.0013724150 2.151843e-02 5.468207e-02 1 163347763 163347938 176 + 2.187 2.273 0.287
ENSG00000143228 E034 167.9226015 0.0002612033 4.566846e-06 3.281156e-05 1 163348945 163349080 136 + 2.093 2.246 0.512
ENSG00000143228 E035 1.1812264 0.1363607034 7.637662e-01 8.470234e-01 1 163354846 163354899 54 + 0.309 0.233 -0.546
ENSG00000143228 E036 151.3951856 0.0018330637 2.129406e-12 4.951025e-11 1 163355335 163355764 430 + 2.020 2.284 0.883