ENSG00000143164

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000312263 ENSG00000143164 HEK293_OSMI2_2hA HEK293_TMG_2hB DCAF6 protein_coding protein_coding 13.36236 8.275452 18.56998 0.9150668 0.2597709 1.165096 7.6333058 4.7197144 9.64347166 0.2694557 0.16881234 1.029295 0.57271250 0.58486667 0.519700000 -0.06516667 0.743926516 0.000185084 FALSE TRUE
ENST00000367843 ENSG00000143164 HEK293_OSMI2_2hA HEK293_TMG_2hB DCAF6 protein_coding protein_coding 13.36236 8.275452 18.56998 0.9150668 0.2597709 1.165096 3.7525695 1.5472545 6.20978593 0.2510358 0.23863858 1.997860 0.26408333 0.18500000 0.334866667 0.14986667 0.000185084 0.000185084 FALSE TRUE
ENST00000489398 ENSG00000143164 HEK293_OSMI2_2hA HEK293_TMG_2hB DCAF6 protein_coding retained_intron 13.36236 8.275452 18.56998 0.9150668 0.2597709 1.165096 0.3768135 1.0535513 0.02158507 1.0535513 0.02158507 -5.073503 0.04234167 0.10426667 0.001133333 -0.10313333 0.829581646 0.000185084 FALSE TRUE
MSTRG.2533.8 ENSG00000143164 HEK293_OSMI2_2hA HEK293_TMG_2hB DCAF6 protein_coding   13.36236 8.275452 18.56998 0.9150668 0.2597709 1.165096 0.9689858 0.7275069 1.78742343 0.4353987 0.50246155 1.285202 0.07461250 0.09886667 0.095766667 -0.00310000 0.884013287 0.000185084 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000143164 E001 0.7427016 0.0156051345 1.004798e-01 1.911857e-01 1 167935783 167936112 330 + 0.321 0.000 -12.527
ENSG00000143164 E002 0.2944980 0.3309556187 5.979392e-01   1 167936274 167936509 236 + 0.158 0.000 -11.201
ENSG00000143164 E003 1.4801046 0.0088891685 1.002767e-01 1.909067e-01 1 167936559 167936660 102 + 0.473 0.171 -2.025
ENSG00000143164 E004 2.2497729 0.0066058760 2.958732e-01 4.370140e-01 1 167936661 167936672 12 + 0.559 0.389 -0.855
ENSG00000143164 E005 2.1025239 0.0068295074 3.750922e-01 5.195059e-01 1 167936673 167936682 10 + 0.532 0.389 -0.729
ENSG00000143164 E006 4.9821796 0.0033106649 2.676257e-02 6.549656e-02 1 167936683 167936707 25 + 0.853 0.534 -1.340
ENSG00000143164 E007 9.8106748 0.0108691130 2.876879e-01 4.282058e-01 1 167936708 167936870 163 + 1.052 0.942 -0.408
ENSG00000143164 E008 31.0137430 0.0127046774 3.381657e-03 1.138398e-02 1 167936871 167937008 138 + 1.557 1.310 -0.850
ENSG00000143164 E009 0.2966881 0.0290785164 4.994489e-01   1 167937153 167937252 100 + 0.158 0.000 -11.202
ENSG00000143164 E010 39.7420856 0.0120225962 1.066139e-02 3.036059e-02 1 167951800 167951861 62 + 1.651 1.467 -0.629
ENSG00000143164 E011 50.3637604 0.0004849630 1.578600e-06 1.255398e-05 1 167966629 167966721 93 + 1.764 1.518 -0.838
ENSG00000143164 E012 0.0000000       1 167968490 167968533 44 +      
ENSG00000143164 E013 0.1482932 0.0407946951 2.319650e-01   1 167969204 167969259 56 + 0.000 0.171 12.216
ENSG00000143164 E014 85.5164006 0.0004649073 9.193701e-07 7.690388e-06 1 167974830 167975015 186 + 1.976 1.789 -0.630
ENSG00000143164 E015 67.1844503 0.0008768663 1.410374e-03 5.340939e-03 1 167987495 167987608 114 + 1.855 1.723 -0.447
ENSG00000143164 E016 72.2463871 0.0006545080 8.157629e-04 3.315680e-03 1 167991204 167991339 136 + 1.886 1.753 -0.447
ENSG00000143164 E017 114.8398202 0.0003108866 4.986904e-05 2.811433e-04 1 167993226 167993440 215 + 2.083 1.959 -0.415
ENSG00000143164 E018 101.1460770 0.0003355340 2.066408e-03 7.430057e-03 1 168002482 168002575 94 + 2.021 1.926 -0.319
ENSG00000143164 E019 113.9303141 0.0013438255 4.905316e-04 2.124400e-03 1 168003870 168003989 120 + 2.078 1.962 -0.391
ENSG00000143164 E020 168.6088811 0.0003128626 7.048572e-05 3.824696e-04 1 168004533 168004793 261 + 2.241 2.146 -0.318
ENSG00000143164 E021 6.2002880 0.0092938221 6.207965e-01 7.378726e-01 1 168015781 168015886 106 + 0.853 0.802 -0.200
ENSG00000143164 E022 3.5515196 0.0069105615 2.761751e-01 4.155887e-01 1 168015887 168015951 65 + 0.694 0.534 -0.702
ENSG00000143164 E023 5.0866879 0.0299868396 5.667185e-01 6.936307e-01 1 168019545 168019667 123 + 0.730 0.833 0.408
ENSG00000143164 E024 1.1395059 0.0511998199 1.272870e-02 3.527419e-02 1 168019668 168019961 294 + 0.086 0.537 3.480
ENSG00000143164 E025 32.5857156 0.0006127876 1.998477e-04 9.630787e-04 1 168022988 168023047 60 + 1.579 1.348 -0.796
ENSG00000143164 E026 2.5894492 0.0097569537 4.398553e-01 5.815915e-01 1 168023048 168024050 1003 + 0.585 0.468 -0.556
ENSG00000143164 E027 101.6256464 0.0003237301 8.219110e-02 1.629646e-01 1 168038371 168038488 118 + 2.007 1.964 -0.145
ENSG00000143164 E028 0.1451727 0.0431761422 1.000000e+00   1 168042730 168043024 295 + 0.086 0.000 -10.201
ENSG00000143164 E029 109.8455167 0.0083764198 2.292121e-01 3.615014e-01 1 168043025 168043140 116 + 2.043 1.992 -0.169
ENSG00000143164 E030 96.5936718 0.0003406866 1.420515e-01 2.508386e-01 1 168044585 168044671 87 + 1.982 1.948 -0.116
ENSG00000143164 E031 155.2657232 0.0002262181 6.323392e-01 7.472135e-01 1 168044900 168045080 181 + 2.164 2.199 0.119
ENSG00000143164 E032 149.0247702 0.0002456305 1.509260e-02 4.069409e-02 1 168045081 168045227 147 + 2.124 2.218 0.312
ENSG00000143164 E033 0.4386386 0.1511841465 1.000000e+00 1.000000e+00 1 168050892 168050933 42 + 0.157 0.170 0.136
ENSG00000143164 E034 123.6826038 0.0003011138 1.831979e-04 8.915508e-04 1 168063621 168063759 139 + 2.023 2.165 0.476
ENSG00000143164 E035 1.0372116 0.0117572104 8.324322e-01 8.950158e-01 1 168063760 168063967 208 + 0.321 0.294 -0.178
ENSG00000143164 E036 129.9879746 0.0003156787 1.291088e-05 8.357573e-05 1 168065590 168065746 157 + 2.039 2.198 0.531
ENSG00000143164 E037 60.3490985 0.0008025283 5.453824e-02 1.171848e-01 1 168066377 168066382 6 + 1.728 1.840 0.378
ENSG00000143164 E038 104.5014863 0.0020765915 2.061334e-04 9.903218e-04 1 168066383 168066465 83 + 1.939 2.110 0.573
ENSG00000143164 E039 119.8664534 0.0045778990 4.911739e-06 3.501992e-05 1 168068358 168068463 106 + 1.972 2.201 0.769
ENSG00000143164 E040 191.6939556 0.0089847441 1.128237e-08 1.377553e-07 1 168075371 168075843 473 + 2.131 2.443 1.043