ENSG00000142731

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000270861 ENSG00000142731 HEK293_OSMI2_2hA HEK293_TMG_2hB PLK4 protein_coding protein_coding 17.8677 3.428904 32.78762 0.209673 0.3867856 3.25357 9.5444966 2.28717595 16.9522060 0.12590487 0.3720071 2.8843905 0.56255000 0.66843333 0.51740000 -0.15103333 0.0125264111 0.0002904005 FALSE TRUE
ENST00000503914 ENSG00000142731 HEK293_OSMI2_2hA HEK293_TMG_2hB PLK4 protein_coding retained_intron 17.8677 3.428904 32.78762 0.209673 0.3867856 3.25357 1.2376100 0.00000000 2.8866689 0.00000000 1.0298074 8.1782510 0.04331667 0.00000000 0.08803333 0.08803333 0.0007086063 0.0002904005 FALSE FALSE
ENST00000507454 ENSG00000142731 HEK293_OSMI2_2hA HEK293_TMG_2hB PLK4 protein_coding retained_intron 17.8677 3.428904 32.78762 0.209673 0.3867856 3.25357 1.6782861 0.08323298 3.5894332 0.04433904 0.2339037 5.2707855 0.10134167 0.02416667 0.10963333 0.08546667 0.0002904005 0.0002904005   FALSE
ENST00000510192 ENSG00000142731 HEK293_OSMI2_2hA HEK293_TMG_2hB PLK4 protein_coding retained_intron 17.8677 3.428904 32.78762 0.209673 0.3867856 3.25357 0.7763434 0.62426590 0.9298403 0.34442722 0.1782108 0.5673278 0.09523333 0.17440000 0.02826667 -0.14613333 0.5131083127 0.0002904005 FALSE TRUE
ENST00000510605 ENSG00000142731 HEK293_OSMI2_2hA HEK293_TMG_2hB PLK4 protein_coding retained_intron 17.8677 3.428904 32.78762 0.209673 0.3867856 3.25357 2.1810065 0.22482679 4.0711287 0.22482679 0.6900745 4.1192993 0.07657083 0.07390000 0.12406667 0.05016667 0.3488987416 0.0002904005 FALSE FALSE
MSTRG.25442.2 ENSG00000142731 HEK293_OSMI2_2hA HEK293_TMG_2hB PLK4 protein_coding   17.8677 3.428904 32.78762 0.209673 0.3867856 3.25357 1.5322882 0.20416472 2.6383592 0.09238381 0.5814258 3.6283060 0.08182500 0.05756667 0.08036667 0.02280000 0.7256948966 0.0002904005 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000142731 E001 0.5932625 0.1559819573 8.764993e-01 9.247407e-01 4 127880139 127880881 743 + 0.178 0.000 -10.481
ENSG00000142731 E002 0.8856842 0.0643551797 4.727095e-01 6.117578e-01 4 127880882 127880892 11 + 0.246 0.000 -11.024
ENSG00000142731 E003 1.3338878 0.0096765512 2.396855e-01 3.737413e-01 4 127880893 127880898 6 + 0.331 0.000 -11.609
ENSG00000142731 E004 7.6772286 0.0022194144 2.640930e-04 1.233308e-03 4 127880899 127880908 10 + 0.880 0.000 -14.140
ENSG00000142731 E005 16.9934495 0.0010388518 8.709342e-05 4.614107e-04 4 127880909 127880926 18 + 1.178 0.545 -2.489
ENSG00000142731 E006 18.9695387 0.0012248728 1.087466e-04 5.623355e-04 4 127880927 127880928 2 + 1.220 0.638 -2.222
ENSG00000142731 E007 92.9159208 0.0003908599 4.802116e-07 4.262305e-06 4 127880929 127881164 236 + 1.869 1.557 -1.058
ENSG00000142731 E008 0.4375944 0.0256889406 1.000000e+00 1.000000e+00 4 127881232 127881370 139 + 0.140 0.000 -10.024
ENSG00000142731 E009 71.9418901 0.0007454266 3.570298e-05 2.082586e-04 4 127881831 127881926 96 + 1.758 1.468 -0.987
ENSG00000142731 E010 34.9844603 0.0021570510 2.278878e-01 3.599102e-01 4 127883262 127883265 4 + 1.435 1.323 -0.388
ENSG00000142731 E011 69.3908628 0.0011365251 5.242457e-02 1.134764e-01 4 127883266 127883357 92 + 1.726 1.596 -0.443
ENSG00000142731 E012 4.6021989 0.1547088694 1.423135e-01 2.511959e-01 4 127883358 127883438 81 + 0.682 0.262 -2.203
ENSG00000142731 E013 64.2253966 0.0003555403 4.870807e-04 2.110757e-03 4 127883439 127883553 115 + 1.706 1.456 -0.854
ENSG00000142731 E014 325.4449909 0.0025538994 5.604540e-03 1.756490e-02 4 127885708 127886626 919 + 2.387 2.285 -0.340
ENSG00000142731 E015 77.1745657 0.0003458651 8.810322e-01 9.277370e-01 4 127886627 127886728 102 + 1.752 1.756 0.012
ENSG00000142731 E016 3.6644151 0.0045817021 4.705664e-01 6.097967e-01 4 127887334 127887395 62 + 0.579 0.427 -0.739
ENSG00000142731 E017 79.9872209 0.0038828952 1.965500e-01 3.219253e-01 4 127887396 127887496 101 + 1.783 1.694 -0.301
ENSG00000142731 E018 130.9066251 0.0040881045 8.198239e-01 8.862631e-01 4 127889866 127890099 234 + 1.985 1.967 -0.061
ENSG00000142731 E019 60.2675452 0.0004767198 6.329229e-01 7.477167e-01 4 127890100 127890129 30 + 1.658 1.622 -0.121
ENSG00000142731 E020 86.7677632 0.0005217799 9.450911e-01 9.695595e-01 4 127890130 127890236 107 + 1.805 1.804 -0.005
ENSG00000142731 E021 7.7684903 0.0020855844 3.820254e-03 1.263704e-02 4 127890997 127891091 95 + 0.873 0.264 -2.952
ENSG00000142731 E022 55.8091078 0.0004088073 7.521615e-01 8.384460e-01 4 127891092 127891115 24 + 1.621 1.596 -0.087
ENSG00000142731 E023 64.8755338 0.0003581112 4.290640e-01 5.717925e-01 4 127891116 127891170 55 + 1.673 1.716 0.143
ENSG00000142731 E024 38.8515953 0.0010044494 1.521005e-01 2.645455e-01 4 127891171 127891196 26 + 1.445 1.547 0.350
ENSG00000142731 E025 71.9564934 0.0003884463 1.515469e-01 2.637730e-01 4 127891579 127891681 103 + 1.711 1.786 0.254
ENSG00000142731 E026 55.4314398 0.0024179913 3.707478e-01 5.151686e-01 4 127892365 127892398 34 + 1.601 1.657 0.189
ENSG00000142731 E027 97.2309403 0.0002774703 7.449771e-01 8.331386e-01 4 127892399 127892514 116 + 1.851 1.862 0.038
ENSG00000142731 E028 12.4212918 0.0079474821 1.525064e-02 4.105301e-02 4 127892515 127892693 179 + 1.042 0.638 -1.585
ENSG00000142731 E029 7.1167673 0.1479353211 8.078705e-01 8.779300e-01 4 127893200 127893284 85 + 0.799 0.718 -0.324
ENSG00000142731 E030 109.5143560 0.0002489271 6.861491e-02 1.410684e-01 4 127893285 127893418 134 + 1.891 1.968 0.258
ENSG00000142731 E031 92.2587269 0.0003375058 2.269842e-02 5.712737e-02 4 127893513 127893604 92 + 1.813 1.918 0.355
ENSG00000142731 E032 117.0253923 0.0017544481 2.493774e-02 6.174422e-02 4 127893734 127893881 148 + 1.916 2.016 0.335
ENSG00000142731 E033 120.6707924 0.0002805009 9.873133e-04 3.915338e-03 4 127894953 127895093 141 + 1.924 2.056 0.443
ENSG00000142731 E034 103.0405115 0.0002785958 8.228331e-04 3.341068e-03 4 127896801 127896907 107 + 1.853 1.998 0.486
ENSG00000142731 E035 142.2530970 0.0021149212 6.633366e-10 1.017548e-08 4 127898439 127899224 786 + 1.968 2.216 0.831