ENSG00000142556

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000270649 ENSG00000142556 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF614 protein_coding protein_coding 4.662119 0.7972551 7.723002 0.05293392 0.2316267 3.259932 3.75260056 0.7181811 6.2537624 0.047910755 0.15022266 3.104660 0.84298750 0.90083333 0.81120000 -0.08963333 0.295249395 0.009313684 FALSE TRUE
ENST00000356322 ENSG00000142556 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF614 protein_coding protein_coding 4.662119 0.7972551 7.723002 0.05293392 0.2316267 3.259932 0.06808881 0.0524627 0.1071633 0.008631173 0.07953582 0.907454 0.03479583 0.06783333 0.01333333 -0.05450000 0.251082946 0.009313684 FALSE TRUE
ENST00000595189 ENSG00000142556 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF614 protein_coding nonsense_mediated_decay 4.662119 0.7972551 7.723002 0.05293392 0.2316267 3.259932 0.82197853 0.0266113 1.3620761 0.013979391 0.19969189 5.227928 0.12038750 0.03133333 0.17546667 0.14413333 0.009313684 0.009313684   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000142556 E001 1.3159143 0.0192040169 5.034293e-03 1.602452e-02 19 52012765 52013190 426 - 0.145 0.722 3.426
ENSG00000142556 E002 0.1451727 0.0432985632 6.393159e-01   19 52013191 52013250 60 - 0.041 0.001 -5.977
ENSG00000142556 E003 0.1451727 0.0432985632 6.393159e-01   19 52013251 52013252 2 - 0.041 0.001 -5.977
ENSG00000142556 E004 317.8886178 0.0067423169 2.742657e-10 4.490822e-09 19 52013324 52017000 3677 - 2.259 2.466 0.693
ENSG00000142556 E005 18.3617921 0.0210120919 2.127714e-01 3.418066e-01 19 52017001 52017095 95 - 1.094 0.979 -0.421
ENSG00000142556 E006 10.6282126 0.0040001331 1.744354e-01 2.939542e-01 19 52017096 52017116 21 - 0.884 0.721 -0.641
ENSG00000142556 E007 26.1840745 0.0077743241 3.705346e-01 5.149706e-01 19 52017117 52017359 243 - 1.227 1.203 -0.088
ENSG00000142556 E008 5.2404752 0.0039702467 3.475236e-01 4.917525e-01 19 52017576 52017697 122 - 0.631 0.496 -0.620
ENSG00000142556 E009 18.6030466 0.0012388718 9.539175e-03 2.763423e-02 19 52018008 52018103 96 - 1.111 0.801 -1.161
ENSG00000142556 E010 14.1105715 0.0013030210 1.753422e-03 6.451234e-03 19 52018368 52018392 25 - 1.010 0.496 -2.115
ENSG00000142556 E011 20.7864667 0.0009883787 2.085890e-05 1.288359e-04 19 52018393 52018494 102 - 1.168 0.496 -2.684
ENSG00000142556 E012 34.7409388 0.0115581170 1.031104e-04 5.364703e-04 19 52025731 52025961 231 - 1.370 0.928 -1.586
ENSG00000142556 E013 18.2640848 0.0195844025 5.699398e-02 1.214494e-01 19 52028242 52028379 138 - 1.097 0.870 -0.844
ENSG00000142556 E014 0.0000000       19 52029856 52030240 385 -