ENSG00000142528

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000270617 ENSG00000142528 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF473 protein_coding protein_coding 7.425078 5.112075 9.564234 0.531172 0.4882788 0.9024288 4.8856215 2.6454992 6.6638024 0.5313946 0.09589133 1.3295258 0.6488083 0.5076667 0.70030000 0.19263333 0.10818284 0.02043068 FALSE TRUE
ENST00000391821 ENSG00000142528 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF473 protein_coding protein_coding 7.425078 5.112075 9.564234 0.531172 0.4882788 0.9024288 0.6783194 0.2932766 0.7893201 0.2215294 0.41738856 1.3981393 0.0922750 0.0536000 0.08726667 0.03366667 0.91832443 0.02043068 FALSE TRUE
ENST00000445728 ENSG00000142528 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF473 protein_coding protein_coding 7.425078 5.112075 9.564234 0.531172 0.4882788 0.9024288 1.1263006 1.7928098 1.1843265 0.1188447 0.44702209 -0.5940499 0.1664250 0.3641333 0.12013333 -0.24400000 0.02043068 0.02043068 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000142528 E001 0.2214452 0.0538919972 2.821654e-01   19 50025714 50025721 8 + 0.000 0.163 10.513
ENSG00000142528 E002 0.5880631 0.5147288533 4.131345e-01 5.566627e-01 19 50025722 50025795 74 + 0.091 0.293 2.053
ENSG00000142528 E003 0.0000000       19 50025806 50025892 87 +      
ENSG00000142528 E004 0.7332358 0.6758945963 6.617581e-01 7.701688e-01 19 50025893 50026004 112 + 0.165 0.296 1.076
ENSG00000142528 E005 13.6557741 0.0012450576 2.041170e-02 5.235564e-02 19 50026005 50026039 35 + 1.214 1.042 -0.619
ENSG00000142528 E006 19.7001680 0.0009194577 6.103692e-03 1.888249e-02 19 50026040 50026057 18 + 1.364 1.192 -0.604
ENSG00000142528 E007 30.7581955 0.0006986270 1.720467e-02 4.539492e-02 19 50026058 50026113 56 + 1.526 1.423 -0.356
ENSG00000142528 E008 22.6071145 0.0013266016 2.673153e-02 6.543406e-02 19 50026114 50026122 9 + 1.402 1.283 -0.412
ENSG00000142528 E009 0.3289534 0.0293397788 6.875651e-01   19 50026123 50026173 51 + 0.091 0.163 0.966
ENSG00000142528 E010 32.2537680 0.0026537308 8.959967e-03 2.617969e-02 19 50030892 50031013 122 + 1.555 1.430 -0.430
ENSG00000142528 E011 41.5748169 0.0005645621 3.307956e-04 1.503845e-03 19 50031014 50031091 78 + 1.673 1.522 -0.514
ENSG00000142528 E012 57.5196302 0.0132396987 3.561272e-02 8.283168e-02 19 50039161 50039287 127 + 1.794 1.698 -0.323
ENSG00000142528 E013 0.8803962 0.0138675860 4.960796e-02 1.084640e-01 19 50040534 50040629 96 + 0.091 0.450 2.967
ENSG00000142528 E014 2.0691101 0.0082793330 4.942117e-01 6.311772e-01 19 50041092 50041729 638 + 0.420 0.571 0.742
ENSG00000142528 E015 53.0459721 0.0025582959 5.254036e-03 1.662177e-02 19 50041730 50041819 90 + 1.760 1.662 -0.333
ENSG00000142528 E016 2.8764586 0.0053026240 3.403485e-01 4.843194e-01 19 50041820 50042784 965 + 0.629 0.515 -0.521
ENSG00000142528 E017 722.7092698 0.0026082254 1.443040e-09 2.075776e-08 19 50044670 50048776 4107 + 2.805 2.912 0.357
ENSG00000142528 E018 0.6265839 0.0202597399 9.664873e-03 2.793984e-02 19 50053190 50053414 225 + 0.000 0.449 14.235