ENSG00000142327

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000270357 ENSG00000142327 HEK293_OSMI2_2hA HEK293_TMG_2hB RNPEPL1 protein_coding protein_coding 9.51161 16.09276 6.171677 1.172999 0.2394105 -1.381238 2.6292076 3.5532467 2.7509000 0.43889531 0.33495238 -0.3680537 0.26858750 0.21920000 0.45010000 0.23090000 1.537707e-02 8.977909e-05 FALSE TRUE
ENST00000460884 ENSG00000142327 HEK293_OSMI2_2hA HEK293_TMG_2hB RNPEPL1 protein_coding retained_intron 9.51161 16.09276 6.171677 1.172999 0.2394105 -1.381238 0.2410918 0.0000000 0.4541401 0.00000000 0.24592523 5.5364886 0.03531667 0.00000000 0.07520000 0.07520000 9.755496e-02 8.977909e-05 FALSE FALSE
ENST00000486058 ENSG00000142327 HEK293_OSMI2_2hA HEK293_TMG_2hB RNPEPL1 protein_coding retained_intron 9.51161 16.09276 6.171677 1.172999 0.2394105 -1.381238 0.9027295 0.5856207 0.4989188 0.09327405 0.05043788 -0.2269585 0.11633750 0.03626667 0.08043333 0.04416667 1.779380e-02 8.977909e-05 FALSE TRUE
ENST00000493398 ENSG00000142327 HEK293_OSMI2_2hA HEK293_TMG_2hB RNPEPL1 protein_coding retained_intron 9.51161 16.09276 6.171677 1.172999 0.2394105 -1.381238 0.4174659 0.4811083 0.4076083 0.24631388 0.09267266 -0.2338908 0.05441250 0.02783333 0.06726667 0.03943333 5.345074e-01 8.977909e-05 TRUE FALSE
ENST00000498042 ENSG00000142327 HEK293_OSMI2_2hA HEK293_TMG_2hB RNPEPL1 protein_coding retained_intron 9.51161 16.09276 6.171677 1.172999 0.2394105 -1.381238 0.4775854 0.2366620 0.2812006 0.16695729 0.15248447 0.2394780 0.06197083 0.01380000 0.04423333 0.03043333 7.272416e-01 8.977909e-05   FALSE
MSTRG.20170.3 ENSG00000142327 HEK293_OSMI2_2hA HEK293_TMG_2hB RNPEPL1 protein_coding   9.51161 16.09276 6.171677 1.172999 0.2394105 -1.381238 0.8198278 1.5235453 0.5207591 0.18754183 0.15309370 -1.5307416 0.07988750 0.09590000 0.08300000 -0.01290000 8.814573e-01 8.977909e-05 FALSE TRUE
MSTRG.20170.5 ENSG00000142327 HEK293_OSMI2_2hA HEK293_TMG_2hB RNPEPL1 protein_coding   9.51161 16.09276 6.171677 1.172999 0.2394105 -1.381238 1.1931119 2.3996098 0.4588877 0.17272309 0.05716556 -2.3614853 0.12274583 0.14963333 0.07420000 -0.07543333 6.009131e-03 8.977909e-05 FALSE TRUE
MSTRG.20170.6 ENSG00000142327 HEK293_OSMI2_2hA HEK293_TMG_2hB RNPEPL1 protein_coding   9.51161 16.09276 6.171677 1.172999 0.2394105 -1.381238 2.3835117 6.2223466 0.1620180 0.11839985 0.10204053 -5.1791441 0.21214167 0.39173333 0.02560000 -0.36613333 8.977909e-05 8.977909e-05 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000142327 E001 0.000000       2 240565804 240565941 138 +      
ENSG00000142327 E002 3.571191 0.0066101067 1.488205e-13 4.127906e-12 2 240568484 240568538 55 + 1.134 0.077 -6.030
ENSG00000142327 E003 8.249900 0.0020222299 4.031631e-23 3.624110e-21 2 240568539 240568860 322 + 1.437 0.334 -4.507
ENSG00000142327 E004 79.035413 0.0003850732 6.102245e-08 6.462001e-07 2 240568861 240569114 254 + 1.964 1.766 -0.667
ENSG00000142327 E005 12.441265 0.0015133397 3.570885e-02 8.302062e-02 2 240570015 240570087 73 + 1.184 1.003 -0.652
ENSG00000142327 E006 10.305636 0.0113054509 8.032325e-02 1.600694e-01 2 240570088 240570136 49 + 1.098 0.924 -0.641
ENSG00000142327 E007 86.840124 0.0066042356 4.574803e-04 1.996505e-03 2 240572423 240572482 60 + 1.977 1.820 -0.528
ENSG00000142327 E008 113.741493 0.0072225092 6.531498e-03 2.000505e-02 2 240572483 240572563 81 + 2.055 1.951 -0.350
ENSG00000142327 E009 145.443975 0.0028426477 4.193462e-01 5.625938e-01 2 240573110 240573261 152 + 2.077 2.083 0.019
ENSG00000142327 E010 104.920240 0.0003041049 7.055682e-01 8.039007e-01 2 240573775 240573869 95 + 1.900 1.952 0.174
ENSG00000142327 E011 73.089359 0.0012931164 6.918428e-01 7.932191e-01 2 240573870 240573891 22 + 1.772 1.788 0.055
ENSG00000142327 E012 7.784454 0.0026791173 2.122911e-03 7.605073e-03 2 240573892 240574112 221 + 1.098 0.766 -1.254
ENSG00000142327 E013 222.162608 0.0003246543 9.250582e-02 1.790343e-01 2 240574113 240574348 236 + 2.274 2.264 -0.034
ENSG00000142327 E014 9.393916 0.0018641939 1.678981e-10 2.852461e-09 2 240574349 240574514 166 + 1.339 0.704 -2.362
ENSG00000142327 E015 124.807184 0.0003374200 9.545643e-02 1.835641e-01 2 240574515 240574553 39 + 2.038 2.013 -0.084
ENSG00000142327 E016 140.045614 0.0003083755 4.202912e-02 9.477450e-02 2 240574554 240574612 59 + 2.095 2.061 -0.113
ENSG00000142327 E017 98.431502 0.0003792306 1.703196e-01 2.885887e-01 2 240574613 240574628 16 + 1.935 1.914 -0.069
ENSG00000142327 E018 4.715574 0.0210925489 3.737509e-03 1.240382e-02 2 240574629 240574721 93 + 0.965 0.568 -1.607
ENSG00000142327 E019 3.207572 0.0059740848 3.327605e-02 7.833901e-02 2 240574722 240574818 97 + 0.774 0.467 -1.354
ENSG00000142327 E020 2.543335 0.0098208131 6.766441e-02 1.394745e-01 2 240574819 240574845 27 + 0.685 0.406 -1.313
ENSG00000142327 E021 5.161826 0.0032621797 1.365206e-03 5.193353e-03 2 240574846 240575029 184 + 0.990 0.591 -1.599
ENSG00000142327 E022 136.377532 0.0002673504 8.739836e-01 9.230067e-01 2 240575030 240575073 44 + 2.024 2.066 0.140
ENSG00000142327 E023 143.882389 0.0002474857 1.392916e-01 2.470822e-01 2 240575074 240575142 69 + 2.089 2.075 -0.048
ENSG00000142327 E024 5.636969 0.0029670101 9.685503e-03 2.798837e-02 2 240575143 240575297 155 + 0.965 0.651 -1.243
ENSG00000142327 E025 6.112075 0.0027753840 2.368899e-01 3.705778e-01 2 240575298 240575501 204 + 0.880 0.751 -0.507
ENSG00000142327 E026 156.066394 0.0036497781 2.260730e-01 3.576851e-01 2 240575502 240575610 109 + 2.044 2.135 0.304
ENSG00000142327 E027 19.402759 0.0120410893 8.783870e-05 4.648912e-04 2 240575611 240576534 924 + 1.462 1.128 -1.168
ENSG00000142327 E028 108.130661 0.0067869314 2.275175e-01 3.594361e-01 2 240576535 240576567 33 + 1.875 1.980 0.353
ENSG00000142327 E029 249.157265 0.0002808267 2.939063e-02 7.075431e-02 2 240576568 240576765 198 + 2.239 2.335 0.321
ENSG00000142327 E030 87.604814 0.0003355205 9.565270e-01 9.767375e-01 2 240576848 240576849 2 + 1.839 1.873 0.113
ENSG00000142327 E031 203.424587 0.0003116547 6.170709e-03 1.906211e-02 2 240576850 240576990 141 + 2.132 2.252 0.404
ENSG00000142327 E032 808.489812 0.0109871960 9.458457e-05 4.966122e-04 2 240577599 240581372 3774 + 2.660 2.869 0.697