ENSG00000142166

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000270139 ENSG00000142166 HEK293_OSMI2_2hA HEK293_TMG_2hB IFNAR1 protein_coding protein_coding 17.7035 5.830939 26.96431 0.2580249 0.6410513 2.207314 10.868338 3.993543 16.013439 0.1682990 0.2903673 2.0008346 0.67189583 0.6867000 0.59406667 -0.09263333 1.641971e-01 1.249979e-08 FALSE TRUE
ENST00000442071 ENSG00000142166 HEK293_OSMI2_2hA HEK293_TMG_2hB IFNAR1 protein_coding protein_coding 17.7035 5.830939 26.96431 0.2580249 0.6410513 2.207314 1.951121 0.000000 3.338798 0.0000000 0.8588593 8.3874995 0.06630417 0.0000000 0.12546667 0.12546667 2.395355e-07 1.249979e-08 FALSE FALSE
ENST00000652513 ENSG00000142166 HEK293_OSMI2_2hA HEK293_TMG_2hB IFNAR1 protein_coding protein_coding 17.7035 5.830939 26.96431 0.2580249 0.6410513 2.207314 1.835951 1.280115 2.069050 0.0897901 0.2888942 0.6884248 0.12782500 0.2196667 0.07643333 -0.14323333 2.464284e-06 1.249979e-08 FALSE TRUE
ENST00000652654 ENSG00000142166 HEK293_OSMI2_2hA HEK293_TMG_2hB IFNAR1 protein_coding nonsense_mediated_decay 17.7035 5.830939 26.96431 0.2580249 0.6410513 2.207314 1.667772 0.000000 2.947242 0.0000000 0.6714518 8.2081083 0.06753750 0.0000000 0.10896667 0.10896667 1.249979e-08 1.249979e-08   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000142166 E001 0.000000       21 33324140 33324172 33 +      
ENSG00000142166 E002 1.524125 0.0128440361 3.005768e-01 4.422923e-01 21 33324173 33324428 256 + 0.409 0.207 -1.357
ENSG00000142166 E003 3.032328 0.0052157062 8.600689e-01 9.137518e-01 21 33324429 33324638 210 + 0.557 0.537 -0.093
ENSG00000142166 E004 0.147249 0.0438653606 1.000000e+00   21 33324639 33324903 265 + 0.070 0.001 -6.448
ENSG00000142166 E005 1.538495 0.1106711470 3.685907e-01 5.130028e-01 21 33324904 33324969 66 + 0.408 0.208 -1.342
ENSG00000142166 E006 2.129681 0.0290255393 1.319877e-01 2.368786e-01 21 33324970 33324971 2 + 0.513 0.207 -1.883
ENSG00000142166 E007 4.193585 0.0186457282 5.839612e-03 1.818939e-02 21 33324972 33324972 1 + 0.755 0.207 -2.939
ENSG00000142166 E008 5.785563 0.0034637070 1.570803e-02 4.208066e-02 21 33324973 33324974 2 + 0.850 0.452 -1.730
ENSG00000142166 E009 24.634019 0.0007446219 2.306916e-02 5.789431e-02 21 33324975 33325004 30 + 1.391 1.211 -0.630
ENSG00000142166 E010 65.869248 0.0003979768 2.134146e-03 7.640122e-03 21 33325005 33325131 127 + 1.800 1.652 -0.501
ENSG00000142166 E011 113.699865 0.0002403690 3.398074e-04 1.539571e-03 21 33335524 33335647 124 + 2.030 1.901 -0.431
ENSG00000142166 E012 112.135576 0.0002952220 9.918175e-03 2.855923e-02 21 33340999 33341077 79 + 2.015 1.927 -0.293
ENSG00000142166 E013 134.268193 0.0003851233 6.837223e-02 1.406779e-01 21 33341078 33341174 97 + 2.083 2.033 -0.170
ENSG00000142166 E014 76.074270 0.0003405971 6.566699e-03 2.009995e-02 21 33343268 33343272 5 + 1.855 1.738 -0.396
ENSG00000142166 E015 151.512490 0.0009025534 8.092693e-02 1.609829e-01 21 33343273 33343422 150 + 2.135 2.088 -0.159
ENSG00000142166 E016 151.948495 0.0003279391 5.564010e-01 6.850491e-01 21 33343535 33343676 142 + 2.125 2.121 -0.014
ENSG00000142166 E017 151.330114 0.0002254598 3.124327e-01 4.549639e-01 21 33345246 33345360 115 + 2.127 2.109 -0.060
ENSG00000142166 E018 14.273658 0.0012517215 3.523217e-12 7.903645e-11 21 33348550 33349077 528 + 0.860 1.498 2.287
ENSG00000142166 E019 178.983321 0.0002182447 8.089572e-01 8.786618e-01 21 33349091 33349290 200 + 2.193 2.201 0.028
ENSG00000142166 E020 161.611640 0.0010733199 3.314065e-01 4.750136e-01 21 33349389 33349543 155 + 2.138 2.187 0.162
ENSG00000142166 E021 168.523416 0.0002095186 7.003022e-01 7.998153e-01 21 33352758 33352908 151 + 2.164 2.191 0.092
ENSG00000142166 E022 161.272217 0.0002674442 1.003168e-01 1.909599e-01 21 33353638 33353783 146 + 2.133 2.200 0.224
ENSG00000142166 E023 88.524481 0.0003548830 5.476800e-01 6.776857e-01 21 33355316 33355330 15 + 1.880 1.921 0.139
ENSG00000142166 E024 967.744607 0.0018034140 2.632860e-03 9.168722e-03 21 33355331 33359864 4534 + 2.909 2.977 0.227