ENSG00000142149

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000270112 ENSG00000142149 HEK293_OSMI2_2hA HEK293_TMG_2hB HUNK protein_coding protein_coding 2.334343 2.517495 3.149172 0.02037827 0.3302599 0.3218382 1.11597795 0.7109656 1.9948356 0.10355054 0.07388067 1.4754817 0.46006250 0.2830667 0.64183333 0.35876667 1.628135e-04 1.273712e-08 FALSE TRUE
ENST00000430354 ENSG00000142149 HEK293_OSMI2_2hA HEK293_TMG_2hB HUNK protein_coding protein_coding 2.334343 2.517495 3.149172 0.02037827 0.3302599 0.3218382 0.03967235 0.0000000 0.2169542 0.00000000 0.21695416 4.5043290 0.01066667 0.0000000 0.05696667 0.05696667 8.306240e-01 1.273712e-08 FALSE TRUE
MSTRG.21227.2 ENSG00000142149 HEK293_OSMI2_2hA HEK293_TMG_2hB HUNK protein_coding   2.334343 2.517495 3.149172 0.02037827 0.3302599 0.3218382 1.15515363 1.7492572 0.9373818 0.08512735 0.04116821 -0.8929486 0.51770833 0.6945000 0.30120000 -0.39330000 1.273712e-08 1.273712e-08 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000142149 E001 20.1297492 0.0010274812 6.403171e-10 9.850276e-09 21 31873020 31873896 877 + 1.493 1.022 -1.662
ENSG00000142149 E002 10.7029387 0.0015641230 1.121923e-02 3.169192e-02 21 31873897 31873935 39 + 1.164 0.939 -0.824
ENSG00000142149 E003 16.6520529 0.0011158671 1.484791e-02 4.014842e-02 21 31924468 31924551 84 + 1.322 1.151 -0.600
ENSG00000142149 E004 29.9493354 0.0077226269 1.232921e-01 2.246652e-01 21 31924552 31924760 209 + 1.524 1.440 -0.290
ENSG00000142149 E005 16.5563103 0.0016297875 6.381414e-02 1.330406e-01 21 31940165 31940220 56 + 1.297 1.173 -0.436
ENSG00000142149 E006 25.3501845 0.0070501583 1.948144e-02 5.037537e-02 21 31946036 31946171 136 + 1.485 1.333 -0.523
ENSG00000142149 E007 22.6284161 0.0010498168 3.480336e-03 1.166745e-02 21 31958843 31958970 128 + 1.455 1.277 -0.618
ENSG00000142149 E008 28.6117785 0.0008080510 1.786994e-01 2.995167e-01 21 31968250 31968385 136 + 1.501 1.448 -0.181
ENSG00000142149 E009 0.2214452 0.0396772218 4.181275e-01   21 31974178 31974554 377 + 0.000 0.136 10.365
ENSG00000142149 E010 31.0773412 0.0009399202 2.575628e-01 3.947041e-01 21 31974555 31974702 148 + 1.528 1.491 -0.125
ENSG00000142149 E011 15.3249034 0.0144962924 6.740426e-01 7.795761e-01 21 31974703 31974717 15 + 1.181 1.257 0.269
ENSG00000142149 E012 23.9635710 0.0009085112 9.579090e-01 9.776140e-01 21 31983526 31983609 84 + 1.383 1.419 0.124
ENSG00000142149 E013 16.2067795 0.0011606712 2.327593e-01 3.657135e-01 21 31990129 31990176 48 + 1.271 1.204 -0.236
ENSG00000142149 E014 32.1917158 0.0006130309 2.711684e-03 9.408626e-03 21 31995768 31995948 181 + 1.590 1.442 -0.506
ENSG00000142149 E015 397.3962428 0.0019208037 2.981847e-13 7.918446e-12 21 31998526 32004064 5539 + 2.526 2.668 0.470
ENSG00000142149 E016 0.4428904 0.5457199740 2.773378e-01 4.168946e-01 21 32044102 32044633 532 + 0.000 0.250 10.165