ENSG00000141543

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000576547 ENSG00000141543 HEK293_OSMI2_2hA HEK293_TMG_2hB EIF4A3 protein_coding protein_coding 210.9855 259.0078 223.5339 23.19522 1.616359 -0.2124934 15.43201 13.60954 21.796359 2.768904 2.549891 0.6790706 0.07280833 0.05446667 0.09763333 0.04316667 2.419299e-01 2.570997e-07 FALSE TRUE
ENST00000647795 ENSG00000141543 HEK293_OSMI2_2hA HEK293_TMG_2hB EIF4A3 protein_coding protein_coding 210.9855 259.0078 223.5339 23.19522 1.616359 -0.2124934 87.55918 53.39170 176.867360 14.468853 4.763401 1.7277917 0.39691250 0.21300000 0.79133333 0.57833333 2.570997e-07 2.570997e-07 FALSE TRUE
MSTRG.15218.3 ENSG00000141543 HEK293_OSMI2_2hA HEK293_TMG_2hB EIF4A3 protein_coding   210.9855 259.0078 223.5339 23.19522 1.616359 -0.2124934 18.84789 30.78923 6.883184 4.207753 1.443751 -2.1596520 0.08895417 0.11786667 0.03076667 -0.08710000 8.490031e-07 2.570997e-07 FALSE TRUE
MSTRG.15218.8 ENSG00000141543 HEK293_OSMI2_2hA HEK293_TMG_2hB EIF4A3 protein_coding   210.9855 259.0078 223.5339 23.19522 1.616359 -0.2124934 81.91346 155.09386 3.069911 30.221675 3.069911 -5.6542020 0.40786250 0.59126667 0.01376667 -0.57750000 4.560271e-03 2.570997e-07 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000141543 E001 38.875377 2.328900e-03 4.886719e-02 1.071411e-01 17 80134369 80135254 886 - 1.520 1.641 0.411
ENSG00000141543 E002 51.634158 1.622568e-03 4.881965e-07 4.325275e-06 17 80135255 80135263 9 - 1.525 1.803 0.946
ENSG00000141543 E003 78.519801 5.649045e-03 1.500990e-08 1.790668e-07 17 80135264 80135298 35 - 1.664 1.992 1.107
ENSG00000141543 E004 296.196172 3.229024e-04 8.743226e-24 8.468190e-22 17 80135299 80135348 50 - 2.307 2.541 0.779
ENSG00000141543 E005 1763.576673 1.471977e-03 1.970593e-16 8.160066e-15 17 80135349 80135506 158 - 3.130 3.287 0.521
ENSG00000141543 E006 20.682784 1.023256e-03 4.280513e-02 9.619006e-02 17 80135507 80136003 497 - 1.419 1.266 -0.534
ENSG00000141543 E007 2165.363338 1.144225e-03 1.435715e-12 3.426455e-11 17 80136004 80136131 128 - 3.248 3.363 0.382
ENSG00000141543 E008 4.210150 1.093729e-02 4.693396e-01 6.085892e-01 17 80136132 80136227 96 - 0.648 0.755 0.447
ENSG00000141543 E009 1882.833463 1.572456e-03 6.522380e-10 1.001739e-08 17 80136228 80136335 108 - 3.186 3.302 0.385
ENSG00000141543 E010 16.334949 6.870227e-03 2.705441e-02 6.610634e-02 17 80136336 80136802 467 - 1.343 1.138 -0.725
ENSG00000141543 E011 15.774578 2.709302e-02 1.800000e-04 8.779955e-04 17 80136803 80137385 583 - 1.432 0.983 -1.595
ENSG00000141543 E012 2061.038062 8.416768e-05 8.537840e-10 1.281061e-08 17 80137386 80137501 116 - 3.274 3.324 0.165
ENSG00000141543 E013 8.209315 4.148975e-03 4.935253e-01 6.305428e-01 17 80137502 80137603 102 - 1.008 0.928 -0.297
ENSG00000141543 E014 2536.297136 6.878030e-04 9.948232e-01 1.000000e+00 17 80138142 80138280 139 - 3.397 3.389 -0.027
ENSG00000141543 E015 5.170125 4.126753e-03 6.670498e-05 3.641803e-04 17 80138281 80139020 740 - 1.039 0.507 -2.167
ENSG00000141543 E016 2284.203957 1.921294e-04 1.973828e-01 3.229514e-01 17 80139021 80139162 142 - 3.360 3.342 -0.059
ENSG00000141543 E017 15.044913 1.152095e-03 1.358383e-05 8.749124e-05 17 80139163 80139487 325 - 1.393 1.017 -1.333
ENSG00000141543 E018 927.191559 2.735748e-04 7.899686e-01 8.656458e-01 17 80139670 80139685 16 - 2.959 2.959 -0.001
ENSG00000141543 E019 1317.762782 1.193942e-04 5.384238e-01 6.698545e-01 17 80139686 80139750 65 - 3.109 3.112 0.008
ENSG00000141543 E020 1896.094936 8.487440e-05 3.663107e-01 5.106435e-01 17 80140008 80140140 133 - 3.276 3.264 -0.041
ENSG00000141543 E021 6.189729 4.415000e-02 1.680817e-02 4.451080e-02 17 80140141 80140476 336 - 1.040 0.684 -1.379
ENSG00000141543 E022 1384.328264 5.061088e-04 1.665412e-01 2.836991e-01 17 80141319 80141381 63 - 3.147 3.119 -0.092
ENSG00000141543 E023 6.497679 2.473112e-03 5.332083e-04 2.284006e-03 17 80141695 80141781 87 - 1.082 0.663 -1.624
ENSG00000141543 E024 1482.692325 7.142387e-05 6.680558e-04 2.781744e-03 17 80141782 80141848 67 - 3.185 3.148 -0.122
ENSG00000141543 E025 1397.803481 4.714429e-04 1.424802e-04 7.143129e-04 17 80144172 80144244 73 - 3.171 3.111 -0.199
ENSG00000141543 E026 1378.831685 2.853963e-03 4.331239e-08 4.730974e-07 17 80146793 80147004 212 - 3.220 3.059 -0.535
ENSG00000141543 E027 261.701366 1.267703e-02 1.972319e-11 3.912565e-10 17 80147005 80147022 18 - 2.617 2.198 -1.398
ENSG00000141543 E028 112.912496 3.812554e-02 3.467018e-05 2.029277e-04 17 80147023 80147060 38 - 2.261 1.826 -1.458
ENSG00000141543 E029 246.751955 1.506920e-02 1.581834e-15 5.827961e-14 17 80147061 80147080 20 - 2.634 2.097 -1.793
ENSG00000141543 E030 178.308628 1.987630e-02 5.701104e-17 2.525268e-15 17 80147081 80147100 20 - 2.528 1.873 -2.191
ENSG00000141543 E031 131.606019 2.123262e-02 1.475657e-17 7.070454e-16 17 80147101 80147132 32 - 2.410 1.706 -2.362
ENSG00000141543 E032 66.196518 2.704287e-02 5.570263e-14 1.642187e-12 17 80147133 80147151 19 - 2.122 1.386 -2.491
ENSG00000141543 E033 2.269959 1.013172e-01 4.732348e-02 1.044054e-01 17 80147404 80147567 164 - 0.717 0.320 -1.950