ENSG00000141522

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000269321 ENSG00000141522 HEK293_OSMI2_2hA HEK293_TMG_2hB ARHGDIA protein_coding protein_coding 457.2328 810.3365 250.9161 34.50341 7.627532 -1.691276 377.647082 732.1927602 161.32635 21.3919145 1.346388 -2.182172 0.77624583 0.904700000 0.64446667 -0.2602333 4.885849e-09 4.885849e-09 FALSE TRUE
ENST00000580033 ENSG00000141522 HEK293_OSMI2_2hA HEK293_TMG_2hB ARHGDIA protein_coding nonsense_mediated_decay 457.2328 810.3365 250.9161 34.50341 7.627532 -1.691276 9.262401 5.8681975 20.94421 2.5573041 2.395225 1.833794 0.03261667 0.007000000 0.08400000 0.0770000 2.299240e-03 4.885849e-09   TRUE
ENST00000583111 ENSG00000141522 HEK293_OSMI2_2hA HEK293_TMG_2hB ARHGDIA protein_coding retained_intron 457.2328 810.3365 250.9161 34.50341 7.627532 -1.691276 12.489093 0.8549399 22.76691 0.6954411 5.494171 4.718829 0.04433333 0.001033333 0.08953333 0.0885000 5.174716e-04 4.885849e-09 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000141522 E001 35.087478 1.251429e-02 5.945803e-01 7.167882e-01 17 81867719 81867720 2 - 1.464 1.406 -0.201
ENSG00000141522 E002 3251.008492 2.279513e-03 2.592756e-08 2.957549e-07 17 81867721 81867896 176 - 3.250 3.384 0.443
ENSG00000141522 E003 2440.309761 2.196006e-03 1.105493e-06 9.093890e-06 17 81867897 81867919 23 - 3.140 3.258 0.391
ENSG00000141522 E004 7714.111609 1.642803e-03 2.276257e-08 2.626249e-07 17 81867920 81868259 340 - 3.652 3.755 0.342
ENSG00000141522 E005 3551.664906 1.291191e-03 6.190192e-04 2.603137e-03 17 81868260 81868339 80 - 3.352 3.413 0.202
ENSG00000141522 E006 2322.053556 9.060556e-04 2.703486e-02 6.606819e-02 17 81868340 81868365 26 - 3.191 3.225 0.114
ENSG00000141522 E007 4064.128078 1.595494e-04 9.851246e-19 5.452203e-17 17 81868366 81868505 140 - 3.393 3.473 0.265
ENSG00000141522 E008 3351.682455 1.626855e-04 2.131564e-23 1.969846e-21 17 81868506 81868584 79 - 3.293 3.392 0.327
ENSG00000141522 E009 2157.923499 9.616041e-05 6.012393e-15 2.046039e-13 17 81868585 81868610 26 - 3.114 3.198 0.279
ENSG00000141522 E010 1845.270415 1.174643e-04 1.232911e-14 4.003622e-13 17 81868611 81868630 20 - 3.041 3.132 0.302
ENSG00000141522 E011 3885.165033 4.880310e-04 3.638174e-13 9.556867e-12 17 81868631 81868869 239 - 3.365 3.455 0.302
ENSG00000141522 E012 2275.022430 4.708108e-05 2.611421e-01 3.987699e-01 17 81868870 81868921 52 - 3.205 3.210 0.015
ENSG00000141522 E013 2315.580548 1.867958e-04 1.552213e-05 9.872164e-05 17 81868922 81868943 22 - 3.264 3.208 -0.187
ENSG00000141522 E014 2924.794514 1.720796e-04 1.133149e-03 4.415022e-03 17 81868944 81868988 45 - 3.353 3.312 -0.135
ENSG00000141522 E015 2863.188748 7.003485e-05 8.935740e-03 2.611736e-02 17 81868989 81869003 15 - 3.335 3.304 -0.106
ENSG00000141522 E016 3366.890756 1.943077e-04 2.292538e-02 5.758993e-02 17 81869004 81869048 45 - 3.405 3.375 -0.099
ENSG00000141522 E017 2358.613817 1.385328e-04 1.219481e-04 6.227336e-04 17 81869049 81869055 7 - 3.266 3.217 -0.163
ENSG00000141522 E018 2591.903818 1.652003e-04 2.431979e-08 2.788760e-07 17 81869056 81869075 20 - 3.322 3.255 -0.222
ENSG00000141522 E019 6.383758 2.613244e-03 6.258923e-03 1.929104e-02 17 81869166 81869172 7 - 1.036 0.664 -1.448
ENSG00000141522 E020 3296.598114 3.172599e-04 1.454416e-06 1.166761e-05 17 81869173 81869236 64 - 3.423 3.361 -0.205
ENSG00000141522 E021 36.415917 5.794307e-04 1.878556e-28 2.765244e-26 17 81869237 81869301 65 - 1.910 1.241 -2.290
ENSG00000141522 E022 28.143631 7.161969e-04 1.565083e-11 3.156945e-10 17 81869302 81869329 28 - 1.689 1.205 -1.669
ENSG00000141522 E023 3881.555680 2.180632e-03 3.686122e-04 1.652800e-03 17 81869330 81869406 77 - 3.516 3.429 -0.290
ENSG00000141522 E024 83.488492 3.224441e-03 1.268206e-15 4.736009e-14 17 81869407 81869437 31 - 2.096 1.686 -1.382
ENSG00000141522 E025 103.738027 1.577018e-02 8.204706e-07 6.940023e-06 17 81869438 81869522 85 - 2.156 1.795 -1.212
ENSG00000141522 E026 89.831977 1.534065e-02 6.598516e-05 3.607390e-04 17 81869523 81869541 19 - 2.050 1.749 -1.011
ENSG00000141522 E027 4103.956607 2.976872e-03 1.870295e-04 9.081216e-04 17 81869542 81869625 84 - 3.554 3.450 -0.345
ENSG00000141522 E028 11.108376 1.690248e-03 1.537076e-02 4.132937e-02 17 81869626 81869740 115 - 1.178 0.899 -1.024
ENSG00000141522 E029 6142.263907 2.097295e-03 3.709837e-08 4.102127e-07 17 81869741 81869957 217 - 3.744 3.621 -0.409
ENSG00000141522 E030 85.837827 5.483040e-03 8.007293e-15 2.672582e-13 17 81869958 81870265 308 - 2.123 1.691 -1.454
ENSG00000141522 E031 386.945810 7.966506e-03 4.791763e-05 2.712073e-04 17 81870636 81871297 662 - 2.606 2.405 -0.672
ENSG00000141522 E032 2476.843408 1.419131e-03 7.842554e-12 1.660982e-10 17 81871298 81871378 81 - 3.365 3.223 -0.473