ENSG00000141258

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000268989 ENSG00000141258 HEK293_OSMI2_2hA HEK293_TMG_2hB SGSM2 protein_coding protein_coding 21.30412 28.86586 13.52964 2.64189 0.3271149 -1.092674 2.1891718 2.8335083 2.2177049 0.50116597 0.06872632 -0.3521139 0.10764167 0.09740000 0.16403333 0.066633333 3.080810e-02 1.408563e-39 FALSE TRUE
ENST00000426855 ENSG00000141258 HEK293_OSMI2_2hA HEK293_TMG_2hB SGSM2 protein_coding protein_coding 21.30412 28.86586 13.52964 2.64189 0.3271149 -1.092674 5.7938641 7.7130278 3.6100209 0.86509152 0.16575249 -1.0931685 0.27111250 0.26953333 0.26706667 -0.002466667 1.000000e+00 1.408563e-39 FALSE TRUE
ENST00000572841 ENSG00000141258 HEK293_OSMI2_2hA HEK293_TMG_2hB SGSM2 protein_coding retained_intron 21.30412 28.86586 13.52964 2.64189 0.3271149 -1.092674 1.9531889 2.9460962 1.0876206 0.84665494 0.49051264 -1.4293137 0.08922917 0.10220000 0.08006667 -0.022133333 8.128384e-01 1.408563e-39 FALSE TRUE
ENST00000572925 ENSG00000141258 HEK293_OSMI2_2hA HEK293_TMG_2hB SGSM2 protein_coding protein_coding 21.30412 28.86586 13.52964 2.64189 0.3271149 -1.092674 3.4848049 8.0717839 0.0000000 0.79825225 0.00000000 -9.6585300 0.12946250 0.28043333 0.00000000 -0.280433333 1.408563e-39 1.408563e-39   FALSE
ENST00000573062 ENSG00000141258 HEK293_OSMI2_2hA HEK293_TMG_2hB SGSM2 protein_coding nonsense_mediated_decay 21.30412 28.86586 13.52964 2.64189 0.3271149 -1.092674 1.3863756 1.1889323 1.1329637 0.50079287 0.12419781 -0.0689706 0.07300833 0.03886667 0.08413333 0.045266667 1.285001e-01 1.408563e-39 TRUE TRUE
ENST00000573717 ENSG00000141258 HEK293_OSMI2_2hA HEK293_TMG_2hB SGSM2 protein_coding retained_intron 21.30412 28.86586 13.52964 2.64189 0.3271149 -1.092674 0.6241238 0.2022362 0.7938874 0.10650489 0.16252541 1.9213231 0.03527917 0.00670000 0.05830000 0.051600000 2.192629e-02 1.408563e-39 FALSE FALSE
ENST00000573851 ENSG00000141258 HEK293_OSMI2_2hA HEK293_TMG_2hB SGSM2 protein_coding protein_coding 21.30412 28.86586 13.52964 2.64189 0.3271149 -1.092674 1.8681266 2.6918843 0.7667597 0.08115884 0.14384966 -1.7984257 0.08517083 0.09436667 0.05680000 -0.037566667 1.604553e-01 1.408563e-39 FALSE FALSE
ENST00000574563 ENSG00000141258 HEK293_OSMI2_2hA HEK293_TMG_2hB SGSM2 protein_coding protein_coding 21.30412 28.86586 13.52964 2.64189 0.3271149 -1.092674 1.0298076 1.2620353 1.0821727 0.30942410 0.08767280 -0.2199377 0.05472500 0.04260000 0.07996667 0.037366667 3.761058e-02 1.408563e-39 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000141258 E001 0.3634088 0.4370616921 5.614694e-01   17 2337498 2337500 3 + 0.000 0.171 9.490
ENSG00000141258 E002 0.3634088 0.4370616921 5.614694e-01   17 2337501 2337505 5 + 0.000 0.171 11.851
ENSG00000141258 E003 0.9545949 0.0176115359 1.135605e-01 2.107221e-01 17 2337506 2337513 8 + 0.442 0.170 -1.884
ENSG00000141258 E004 3.5481968 0.0084831384 1.726894e-04 8.467835e-04 17 2337514 2337598 85 + 0.930 0.387 -2.388
ENSG00000141258 E005 5.1046705 0.0035729763 2.660777e-03 9.254880e-03 17 2337599 2337611 13 + 0.973 0.588 -1.546
ENSG00000141258 E006 6.3957049 0.0310647981 2.639912e-02 6.474342e-02 17 2337612 2337617 6 + 1.013 0.709 -1.175
ENSG00000141258 E007 7.9499145 0.0342210524 1.036324e-01 1.960266e-01 17 2337618 2337621 4 + 1.049 0.833 -0.809
ENSG00000141258 E008 33.5171660 0.0006392850 6.503572e-04 2.717309e-03 17 2337622 2337745 124 + 1.620 1.426 -0.665
ENSG00000141258 E009 48.1945873 0.0009189383 3.186098e-04 1.455292e-03 17 2343545 2343620 76 + 1.764 1.590 -0.590
ENSG00000141258 E010 72.5645515 0.0004263579 7.826768e-04 3.197459e-03 17 2361637 2361776 140 + 1.909 1.780 -0.434
ENSG00000141258 E011 39.8104266 0.0005117248 2.666003e-02 6.528357e-02 17 2361777 2361799 23 + 1.647 1.535 -0.380
ENSG00000141258 E012 70.7323247 0.0003753258 6.679173e-01 7.750539e-01 17 2362109 2362270 162 + 1.817 1.813 -0.012
ENSG00000141258 E013 51.2156646 0.0004494797 6.025343e-01 7.234971e-01 17 2362838 2362905 68 + 1.647 1.690 0.146
ENSG00000141258 E014 17.7357685 0.0367395874 2.042125e-01 3.314437e-01 17 2362906 2362988 83 + 1.311 1.190 -0.429
ENSG00000141258 E015 87.7135507 0.0003772658 2.424128e-01 3.770010e-01 17 2362989 2363134 146 + 1.927 1.896 -0.105
ENSG00000141258 E016 76.5962514 0.0245061812 9.933378e-01 9.999939e-01 17 2363465 2363561 97 + 1.838 1.859 0.070
ENSG00000141258 E017 62.2818318 0.0184175927 6.261310e-01 7.421272e-01 17 2363562 2363599 38 + 1.773 1.757 -0.053
ENSG00000141258 E018 2.8283964 0.0668502272 7.616902e-02 1.533833e-01 17 2363845 2363999 155 + 0.734 0.431 -1.381
ENSG00000141258 E019 3.3359075 0.0793538118 6.504825e-02 1.351168e-01 17 2364000 2364058 59 + 0.799 0.470 -1.440
ENSG00000141258 E020 85.1687119 0.0120179512 6.129671e-01 7.317851e-01 17 2364059 2364183 125 + 1.903 1.893 -0.034
ENSG00000141258 E021 8.9980503 0.0019118838 2.209580e-04 1.053581e-03 17 2364184 2364595 412 + 1.193 0.814 -1.405
ENSG00000141258 E022 62.9282381 0.0029593881 1.733890e-02 4.568698e-02 17 2364596 2364663 68 + 1.834 1.727 -0.360
ENSG00000141258 E023 4.4673771 0.0037931569 3.792778e-04 1.694487e-03 17 2364664 2364896 233 + 0.973 0.499 -1.962
ENSG00000141258 E024 84.0107246 0.0067710295 1.611911e-01 2.766549e-01 17 2364897 2365057 161 + 1.929 1.870 -0.197
ENSG00000141258 E025 81.4497910 0.0063565100 1.395636e-01 2.474280e-01 17 2365215 2365341 127 + 1.920 1.856 -0.216
ENSG00000141258 E026 7.0889713 0.0023434413 4.007686e-02 9.117201e-02 17 2366528 2367270 743 + 1.012 0.781 -0.883
ENSG00000141258 E027 23.0918064 0.0008831409 7.123005e-02 1.453822e-01 17 2367271 2367405 135 + 1.418 1.300 -0.412
ENSG00000141258 E028 82.5445730 0.0012565897 1.758519e-01 2.957907e-01 17 2371262 2371415 154 + 1.911 1.868 -0.145
ENSG00000141258 E029 2.1476770 0.0081787402 9.004403e-05 4.752088e-04 17 2371416 2371820 405 + 0.800 0.170 -3.469
ENSG00000141258 E030 1.7381807 0.0233981252 1.107485e-01 2.066297e-01 17 2371863 2372189 327 + 0.563 0.293 -1.462
ENSG00000141258 E031 54.9209655 0.0006350541 6.112240e-01 7.303761e-01 17 2372190 2372254 65 + 1.715 1.704 -0.035
ENSG00000141258 E032 7.9264055 0.0479889402 1.329686e-01 2.382441e-01 17 2372255 2372342 88 + 1.048 0.835 -0.803
ENSG00000141258 E033 81.9980725 0.0020569669 1.811651e-01 3.026799e-01 17 2372343 2372488 146 + 1.906 1.862 -0.148
ENSG00000141258 E034 10.2806667 0.0017098612 1.028482e-04 5.352236e-04 17 2372489 2372580 92 + 1.240 0.859 -1.393
ENSG00000141258 E035 27.5126236 0.0006907987 2.380326e-10 3.932894e-09 17 2372581 2372952 372 + 1.651 1.250 -1.384
ENSG00000141258 E036 104.3537016 0.0034478875 3.491831e-01 4.934006e-01 17 2372953 2373081 129 + 1.996 1.974 -0.076
ENSG00000141258 E037 10.4031046 0.0239796561 1.392702e-07 1.376550e-06 17 2373082 2373330 249 + 1.347 0.709 -2.367
ENSG00000141258 E038 116.7304581 0.0105971737 9.039191e-01 9.428700e-01 17 2373331 2373513 183 + 2.010 2.041 0.106
ENSG00000141258 E039 48.6854026 0.0074958221 3.903805e-16 1.555032e-14 17 2373514 2374580 1067 + 1.941 1.431 -1.732
ENSG00000141258 E040 190.0444755 0.0006077705 9.355479e-01 9.634280e-01 17 2375492 2375875 384 + 2.234 2.247 0.046
ENSG00000141258 E041 46.3102924 0.0005189510 6.536329e-01 7.640593e-01 17 2376137 2376140 4 + 1.647 1.637 -0.031
ENSG00000141258 E042 112.1850569 0.0005912236 7.634211e-01 8.467275e-01 17 2376141 2376261 121 + 2.013 2.018 0.016
ENSG00000141258 E043 4.2261602 0.0367850476 8.012538e-02 1.597440e-01 17 2376360 2376732 373 + 0.857 0.588 -1.110
ENSG00000141258 E044 114.1078294 0.0011281556 6.400620e-01 7.533788e-01 17 2376733 2376815 83 + 1.998 2.033 0.115
ENSG00000141258 E045 149.2361434 0.0003739448 2.956604e-01 4.368121e-01 17 2376959 2377068 110 + 2.104 2.153 0.164
ENSG00000141258 E046 121.3990941 0.0002795709 1.078880e-01 2.024049e-01 17 2377857 2377912 56 + 1.998 2.070 0.240
ENSG00000141258 E047 102.9880159 0.0003195964 7.520593e-01 8.383765e-01 17 2377913 2377953 41 + 1.960 1.987 0.092
ENSG00000141258 E048 168.3483267 0.0002615751 2.112792e-02 5.386744e-02 17 2379036 2379167 132 + 2.130 2.215 0.283
ENSG00000141258 E049 101.1596010 0.0031786812 1.923001e-01 3.166480e-01 17 2379168 2379203 36 + 1.920 1.998 0.260
ENSG00000141258 E050 24.5091482 0.0013240569 4.208143e-03 1.372624e-02 17 2379204 2379431 228 + 1.193 1.437 0.853
ENSG00000141258 E051 115.1508258 0.0051407939 4.943412e-02 1.081563e-01 17 2379432 2379493 62 + 1.947 2.063 0.390
ENSG00000141258 E052 344.4148094 0.0030996233 1.650145e-06 1.306755e-05 17 2379494 2380264 771 + 2.373 2.550 0.592
ENSG00000141258 E053 413.0611475 0.0039514808 4.757620e-10 7.475466e-09 17 2380265 2381058 794 + 2.408 2.642 0.778