ENSG00000140650

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000268261 ENSG00000140650 HEK293_OSMI2_2hA HEK293_TMG_2hB   protein_coding protein_coding 29.0547 39.2757 29.47591 2.181289 0.5226597 -0.4139785 20.4350222 31.026245 18.567558 2.1849936 0.4178238 -0.7403931 0.68815000 0.78866667 0.6298 -0.15886667 3.136562e-07 1.516358e-16 FALSE TRUE
ENST00000566604 ENSG00000140650 HEK293_OSMI2_2hA HEK293_TMG_2hB   protein_coding nonsense_mediated_decay 29.0547 39.2757 29.47591 2.181289 0.5226597 -0.4139785 0.8431042 0.000000 2.140524 0.0000000 0.4343727 7.7485446 0.03170833 0.00000000 0.0731 0.07310000 1.516358e-16 1.516358e-16 TRUE FALSE
ENST00000682008 ENSG00000140650 HEK293_OSMI2_2hA HEK293_TMG_2hB   protein_coding protein_coding 29.0547 39.2757 29.47591 2.181289 0.5226597 -0.4139785 2.8251915 1.980304 3.867686 0.1658708 0.6493800 0.9622070 0.10391667 0.05063333 0.1316 0.08096667 3.332421e-03 1.516358e-16 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000140650 E001 0.1515154 0.0432797006 3.320636e-01   16 8788823 8788839 17 + 0.142 0.000 -9.852
ENSG00000140650 E002 0.2998086 0.0291118637 7.593920e-01   16 8788840 8789011 172 + 0.142 0.105 -0.508
ENSG00000140650 E003 0.0000000       16 8795210 8795662 453 +      
ENSG00000140650 E004 2.0961786 0.0073873622 3.126155e-01 4.551392e-01 16 8797716 8797809 94 + 0.570 0.421 -0.727
ENSG00000140650 E005 9.2768812 0.0019604043 4.416674e-03 1.430985e-02 16 8797810 8797820 11 + 1.152 0.878 -1.007
ENSG00000140650 E006 11.4418568 0.0016698380 1.125715e-02 3.177866e-02 16 8797821 8797827 7 + 1.207 0.989 -0.790
ENSG00000140650 E007 27.0844684 0.0055221759 9.501969e-06 6.348787e-05 16 8797828 8797836 9 + 1.599 1.298 -1.039
ENSG00000140650 E008 27.5649372 0.0044090421 4.029215e-06 2.928198e-05 16 8797837 8797837 1 + 1.608 1.304 -1.047
ENSG00000140650 E009 35.7785018 0.0012186075 2.722011e-05 1.635667e-04 16 8797838 8797838 1 + 1.677 1.456 -0.753
ENSG00000140650 E010 45.5058784 0.0059340343 1.488533e-02 4.023256e-02 16 8797839 8797841 3 + 1.730 1.602 -0.432
ENSG00000140650 E011 52.5868410 0.0005446813 6.692289e-05 3.652543e-04 16 8797842 8797849 8 + 1.813 1.648 -0.558
ENSG00000140650 E012 79.9635222 0.0030278979 1.138954e-04 5.856508e-04 16 8797850 8797866 17 + 1.985 1.833 -0.513
ENSG00000140650 E013 140.5366392 0.0015181369 5.365500e-07 4.717930e-06 16 8797867 8797911 45 + 2.222 2.077 -0.485
ENSG00000140650 E014 157.1754245 0.0002690969 6.814805e-08 7.149828e-07 16 8797912 8797948 37 + 2.259 2.134 -0.418
ENSG00000140650 E015 247.5219058 0.0028303714 4.963524e-04 2.146076e-03 16 8801799 8801910 112 + 2.433 2.345 -0.295
ENSG00000140650 E016 0.2214452 0.0454323917 6.958907e-01   16 8802081 8802145 65 + 0.000 0.105 10.281
ENSG00000140650 E017 0.5483223 0.0218232142 8.257477e-01 8.902843e-01 16 8802146 8802260 115 + 0.142 0.190 0.498
ENSG00000140650 E018 0.6213751 0.0240406042 1.598454e-01 2.749144e-01 16 8802261 8802289 29 + 0.335 0.105 -2.089
ENSG00000140650 E019 1.1008118 0.0112325701 3.589526e-01 5.032897e-01 16 8802290 8802389 100 + 0.407 0.260 -0.920
ENSG00000140650 E020 189.0835464 0.0008230892 3.277556e-02 7.735819e-02 16 8804767 8804843 77 + 2.284 2.251 -0.112
ENSG00000140650 E021 209.9949930 0.0016679704 7.459136e-01 8.338118e-01 16 8806316 8806407 92 + 2.298 2.315 0.056
ENSG00000140650 E022 6.9550277 0.0691104037 1.636187e-01 2.798480e-01 16 8806408 8807034 627 + 0.997 0.810 -0.707
ENSG00000140650 E023 54.9266991 0.0063011261 9.451326e-04 3.772220e-03 16 8807564 8811078 3515 + 1.830 1.656 -0.592
ENSG00000140650 E024 183.1369204 0.0002316428 9.796768e-01 9.913460e-01 16 8811079 8811178 100 + 2.233 2.263 0.098
ENSG00000140650 E025 111.9677811 0.0003340727 6.867202e-01 7.891751e-01 16 8811638 8811641 4 + 2.033 2.049 0.051
ENSG00000140650 E026 202.9624932 0.0001906285 1.976509e-01 3.232845e-01 16 8811642 8811713 72 + 2.300 2.296 -0.012
ENSG00000140650 E027 240.9404668 0.0001741112 3.085722e-01 4.508567e-01 16 8812991 8813106 116 + 2.367 2.372 0.017
ENSG00000140650 E028 2.0991973 0.0305050969 5.413515e-01 6.723490e-01 16 8813107 8813385 279 + 0.407 0.537 0.658
ENSG00000140650 E029 0.8416995 0.0238184355 5.072046e-02 1.104490e-01 16 8829193 8829258 66 + 0.000 0.374 12.770
ENSG00000140650 E030 0.9211812 0.0136968632 4.840035e-02 1.063292e-01 16 8842207 8842379 173 + 0.000 0.374 12.919
ENSG00000140650 E031 860.1739057 0.0010897942 8.496459e-09 1.061130e-07 16 8847724 8848765 1042 + 2.839 2.964 0.416
ENSG00000140650 E032 554.8458056 0.0029820294 1.148187e-13 3.234989e-12 16 8848766 8849325 560 + 2.568 2.804 0.786
ENSG00000140650 E033 5.4678612 0.0331516844 9.655921e-01 9.823726e-01 16 8849326 8849338 13 + 0.781 0.794 0.051
ENSG00000140650 E034 6.3180710 0.0352422115 3.105064e-01 4.529142e-01 16 8849339 8849368 30 + 0.923 0.794 -0.499
ENSG00000140650 E035 18.4401383 0.0202435060 4.422848e-01 5.839357e-01 16 8849369 8849655 287 + 1.301 1.245 -0.196
ENSG00000140650 E036 15.1599769 0.0223605922 8.304813e-01 8.936485e-01 16 8849849 8849993 145 + 1.163 1.207 0.155
ENSG00000140650 E037 12.8076529 0.0284021472 2.210553e-01 3.516784e-01 16 8849994 8850932 939 + 1.197 1.069 -0.458
ENSG00000140650 E038 3.8194096 0.0047025910 1.610809e-01 2.765307e-01 16 8850933 8851050 118 + 0.781 0.602 -0.746
ENSG00000140650 E039 5.2639235 0.0031516719 9.095429e-02 1.766464e-01 16 8853312 8853525 214 + 0.902 0.707 -0.768
ENSG00000140650 E040 6.7847339 0.0026582063 6.810999e-01 7.848921e-01 16 8853526 8853636 111 + 0.902 0.878 -0.091
ENSG00000140650 E041 2.5486471 0.0099420491 3.036724e-01 4.456291e-01 16 8854264 8854356 93 + 0.613 0.465 -0.695
ENSG00000140650 E042 17.6077442 0.0043637823 2.202184e-01 3.506792e-01 16 8855034 8855217 184 + 1.163 1.298 0.476
ENSG00000140650 E043 3.8624850 0.0160086206 2.659598e-01 4.040844e-01 16 8855218 8855990 773 + 0.752 0.604 -0.625
ENSG00000140650 E044 2.3355206 0.0105930387 2.948172e-01 4.359198e-01 16 8855991 8856412 422 + 0.613 0.464 -0.701
ENSG00000140650 E045 6.7677649 0.0191142170 2.424203e-01 3.770088e-01 16 8859813 8859946 134 + 0.752 0.937 0.718
ENSG00000140650 E046 10.2643971 0.0016816717 2.917629e-02 7.034234e-02 16 8859947 8860127 181 + 0.857 1.123 0.986
ENSG00000140650 E047 14.4629182 0.0012834564 2.407610e-02 5.996952e-02 16 8860128 8860536 409 + 1.013 1.254 0.864
ENSG00000140650 E048 7.2211638 0.0026167016 1.363342e-01 2.429440e-01 16 8862026 8862534 509 + 0.752 0.964 0.818