ENSG00000140600

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000427482 ENSG00000140600 HEK293_OSMI2_2hA HEK293_TMG_2hB SH3GL3 protein_coding protein_coding 9.147492 8.626904 13.44644 0.4355248 0.5617246 0.6397105 6.9428980 5.6756983 11.1259270 0.6453245 0.6612633 0.9698122 0.74211250 0.65506667 0.82660000 0.1715333 0.032242997 0.006965852 FALSE TRUE
ENST00000563901 ENSG00000140600 HEK293_OSMI2_2hA HEK293_TMG_2hB SH3GL3 protein_coding nonsense_mediated_decay 9.147492 8.626904 13.44644 0.4355248 0.5617246 0.6397105 0.7505021 0.4936155 1.3307595 0.1353254 0.3353004 1.4126558 0.08395417 0.05723333 0.09773333 0.0405000 0.463824469 0.006965852 FALSE TRUE
ENST00000564054 ENSG00000140600 HEK293_OSMI2_2hA HEK293_TMG_2hB SH3GL3 protein_coding processed_transcript 9.147492 8.626904 13.44644 0.4355248 0.5617246 0.6397105 1.1281213 2.1949472 0.5197311 0.3484713 0.2656581 -2.0574119 0.13778333 0.25773333 0.04046667 -0.2172667 0.006965852 0.006965852   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000140600 E001 0.000000       15 83447228 83447340 113 +      
ENSG00000140600 E002 3.591149 0.0043051597 1.881932e-03 6.858650e-03 15 83447341 83447375 35 + 0.800 0.273 -2.599
ENSG00000140600 E003 23.684638 0.0007880704 6.399265e-11 1.165134e-09 15 83447376 83447530 155 + 1.532 0.990 -1.915
ENSG00000140600 E004 25.637781 0.0008060226 4.686253e-06 3.357016e-05 15 83447531 83447578 48 + 1.526 1.189 -1.172
ENSG00000140600 E005 0.000000       15 83488061 83488296 236 +      
ENSG00000140600 E006 0.000000       15 83489189 83489314 126 +      
ENSG00000140600 E007 2.801204 0.0528590366 2.800316e-01 4.198313e-01 15 83490752 83490902 151 + 0.641 0.441 -0.943
ENSG00000140600 E008 40.834149 0.0005941182 6.190994e-06 4.317083e-05 15 83559253 83559321 69 + 1.701 1.443 -0.881
ENSG00000140600 E009 51.696003 0.0014549982 2.613812e-07 2.448593e-06 15 83565134 83565206 73 + 1.805 1.530 -0.933
ENSG00000140600 E010 0.294498 0.4171398555 5.377701e-01   15 83565207 83565883 677 + 0.171 0.000 -9.268
ENSG00000140600 E011 32.330915 0.0038175491 5.706983e-05 3.169102e-04 15 83568529 83568535 7 + 1.608 1.326 -0.973
ENSG00000140600 E012 75.958692 0.0119389228 1.907913e-01 3.147381e-01 15 83568536 83568672 137 + 1.909 1.834 -0.254
ENSG00000140600 E013 68.656415 0.0040325095 1.017879e-01 1.932245e-01 15 83572565 83572698 134 + 1.802 1.887 0.286
ENSG00000140600 E014 82.010855 0.0004249480 6.959160e-01 7.963785e-01 15 83576583 83576741 159 + 1.903 1.924 0.070
ENSG00000140600 E015 70.328575 0.0061729902 5.027318e-01 6.387429e-01 15 83586983 83587086 104 + 1.830 1.871 0.139
ENSG00000140600 E016 92.131335 0.0071577049 4.136757e-01 5.570979e-01 15 83588662 83588771 110 + 1.944 1.993 0.166
ENSG00000140600 E017 6.012866 0.0027917064 2.683974e-01 4.068515e-01 15 83609270 83609402 133 + 0.783 0.927 0.555
ENSG00000140600 E018 3.391266 0.0076512223 2.807579e-01 4.206373e-01 15 83609403 83609466 64 + 0.563 0.731 0.723
ENSG00000140600 E019 1.496024 0.3388063640 4.785874e-01 6.170001e-01 15 83611418 83611587 170 + 0.469 0.267 -1.195
ENSG00000140600 E020 213.875046 0.0007061607 3.581253e-19 2.080520e-17 15 83618082 83618743 662 + 2.230 2.438 0.693