ENSG00000140577

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000268184 ENSG00000140577 HEK293_OSMI2_2hA HEK293_TMG_2hB CRTC3 protein_coding protein_coding 13.08328 16.43159 10.71909 1.074247 0.4421817 -0.6158225 7.2861569 10.3108718 6.273693 0.8989455 0.5017322 -0.7158802 0.54701667 0.62566667 0.5835000 -0.04216667 6.635021e-01 3.201688e-22 FALSE TRUE
ENST00000420329 ENSG00000140577 HEK293_OSMI2_2hA HEK293_TMG_2hB CRTC3 protein_coding protein_coding 13.08328 16.43159 10.71909 1.074247 0.4421817 -0.6158225 2.1940934 0.2265451 2.335734 0.2265451 0.4285039 3.3098520 0.17482917 0.01243333 0.2221333 0.20970000 1.468183e-02 3.201688e-22 FALSE TRUE
ENST00000692149 ENSG00000140577 HEK293_OSMI2_2hA HEK293_TMG_2hB CRTC3 protein_coding nonsense_mediated_decay 13.08328 16.43159 10.71909 1.074247 0.4421817 -0.6158225 0.9644633 4.0386042 0.000000 0.3491929 0.0000000 -8.6612808 0.06182917 0.24956667 0.0000000 -0.24956667 3.201688e-22 3.201688e-22 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000140577 E001 1.4790604 0.0089599787 2.720372e-05 1.634889e-04 15 90529856 90529922 67 + 0.693 0.000 -12.419
ENSG00000140577 E002 1.3338878 0.0101576227 7.325709e-05 3.958453e-04 15 90529923 90529924 2 + 0.657 0.000 -14.715
ENSG00000140577 E003 2.8192884 0.0066758304 1.164688e-04 5.976991e-04 15 90529925 90529934 10 + 0.839 0.257 -2.868
ENSG00000140577 E004 7.2928851 0.0351257106 9.032075e-08 9.243074e-07 15 90529935 90529952 18 + 1.224 0.462 -3.054
ENSG00000140577 E005 7.9947991 0.0432751456 9.386802e-07 7.833927e-06 15 90529953 90529957 5 + 1.244 0.538 -2.755
ENSG00000140577 E006 12.4357979 0.0057876917 1.619474e-09 2.309579e-08 15 90529958 90529967 10 + 1.377 0.806 -2.079
ENSG00000140577 E007 14.6877699 0.0464211275 5.555236e-05 3.094178e-04 15 90529968 90529974 7 + 1.418 0.927 -1.759
ENSG00000140577 E008 21.3694917 0.0156516387 2.722379e-06 2.057390e-05 15 90529975 90530013 39 + 1.537 1.139 -1.390
ENSG00000140577 E009 25.9023523 0.0041199262 7.027363e-07 6.031136e-06 15 90530014 90530062 49 + 1.584 1.262 -1.113
ENSG00000140577 E010 18.6643086 0.0019247317 1.936391e-02 5.012276e-02 15 90530063 90530071 9 + 1.362 1.214 -0.520
ENSG00000140577 E011 68.6451148 0.0006699734 5.819508e-04 2.467024e-03 15 90530072 90530203 132 + 1.888 1.778 -0.370
ENSG00000140577 E012 0.5202097 0.0208103719 3.292477e-01 4.727527e-01 15 90530532 90530747 216 + 0.252 0.104 -1.545
ENSG00000140577 E013 0.2934659 0.0302239965 7.300615e-01   15 90539617 90540038 422 + 0.144 0.103 -0.549
ENSG00000140577 E014 55.2634992 0.0033592212 8.825236e-01 9.288001e-01 15 90540039 90540081 43 + 1.704 1.738 0.115
ENSG00000140577 E015 65.4853770 0.0003982603 9.001940e-01 9.403136e-01 15 90540082 90540137 56 + 1.770 1.816 0.155
ENSG00000140577 E016 73.8735609 0.0060661326 9.349229e-01 9.630394e-01 15 90593636 90593711 76 + 1.824 1.870 0.156
ENSG00000140577 E017 46.4319463 0.0171873879 8.477026e-01 9.054331e-01 15 90593712 90593712 1 + 1.618 1.677 0.200
ENSG00000140577 E018 63.8674821 0.0080571587 9.209380e-01 9.541154e-01 15 90593713 90593755 43 + 1.769 1.806 0.125
ENSG00000140577 E019 33.4591594 0.0152074848 9.169272e-02 1.777785e-01 15 90593756 90595577 1822 + 1.584 1.486 -0.335
ENSG00000140577 E020 0.5985731 0.0273497260 1.436523e-01 2.529769e-01 15 90597843 90597873 31 + 0.339 0.103 -2.135
ENSG00000140577 E021 0.5922303 0.0179968321 1.415808e-01 2.501929e-01 15 90597874 90597928 55 + 0.338 0.103 -2.133
ENSG00000140577 E022 1.3200789 0.0120170652 4.290830e-01 5.718099e-01 15 90597929 90598211 283 + 0.252 0.417 1.037
ENSG00000140577 E023 1.1426289 0.0111814453 3.225684e-01 4.658704e-01 15 90598346 90598419 74 + 0.411 0.257 -0.962
ENSG00000140577 E024 7.5127620 0.0021826211 3.108449e-02 7.407032e-02 15 90598450 90599535 1086 + 1.035 0.823 -0.798
ENSG00000140577 E025 2.6486535 0.0057887293 3.543606e-01 4.986485e-01 15 90600308 90600441 134 + 0.618 0.499 -0.546
ENSG00000140577 E026 0.6653823 0.0172671820 1.419844e-01 2.507529e-01 15 90600442 90600646 205 + 0.338 0.104 -2.130
ENSG00000140577 E027 29.4769108 0.0108388505 4.511597e-01 5.921438e-01 15 90602324 90602334 11 + 1.473 1.454 -0.063
ENSG00000140577 E028 37.9839899 0.0167503587 4.417218e-01 5.833672e-01 15 90602335 90602385 51 + 1.579 1.561 -0.061
ENSG00000140577 E029 41.8002486 0.0093160032 8.273533e-02 1.638336e-01 15 90604385 90604447 63 + 1.661 1.581 -0.271
ENSG00000140577 E030 56.4046198 0.0004616217 1.106352e-05 7.276850e-05 15 90607378 90607478 101 + 1.837 1.670 -0.563
ENSG00000140577 E031 0.6224193 0.0167483761 6.408772e-01 7.540288e-01 15 90613657 90614049 393 + 0.252 0.187 -0.548
ENSG00000140577 E032 42.4633249 0.0081213052 3.695017e-04 1.656122e-03 15 90614453 90614488 36 + 1.736 1.532 -0.693
ENSG00000140577 E033 20.9516896 0.0008878241 1.528057e-02 4.112422e-02 15 90617883 90617885 3 + 1.405 1.261 -0.500
ENSG00000140577 E034 56.4403376 0.0009807231 4.469600e-08 4.867792e-07 15 90617886 90617968 83 + 1.865 1.642 -0.755
ENSG00000140577 E035 0.8815421 0.0159638415 9.417120e-01 9.673501e-01 15 90617969 90618206 238 + 0.252 0.257 0.040
ENSG00000140577 E036 47.5084994 0.0004979604 7.336731e-06 5.026841e-05 15 90619741 90619790 50 + 1.778 1.591 -0.636
ENSG00000140577 E037 0.0000000       15 90624907 90625062 156 +      
ENSG00000140577 E038 108.2148021 0.0002768428 2.172312e-09 3.024013e-08 15 90625776 90625993 218 + 2.115 1.952 -0.546
ENSG00000140577 E039 112.8624226 0.0002637142 9.865682e-06 6.567657e-05 15 90629234 90629532 299 + 2.102 1.995 -0.360
ENSG00000140577 E040 0.5879639 0.0217831374 1.429565e-01 2.520679e-01 15 90637852 90638338 487 + 0.338 0.103 -2.134
ENSG00000140577 E041 81.5102295 0.0003387917 1.347210e-04 6.800015e-04 15 90638446 90638646 201 + 1.963 1.853 -0.370
ENSG00000140577 E042 66.1946917 0.0003640127 7.697858e-04 3.149142e-03 15 90638735 90638815 81 + 1.872 1.766 -0.359
ENSG00000140577 E043 86.1614672 0.0013242982 1.206033e-03 4.660940e-03 15 90641097 90641199 103 + 1.974 1.881 -0.313
ENSG00000140577 E044 35.4042323 0.0006520860 1.326482e-02 3.653651e-02 15 90641932 90641934 3 + 1.601 1.494 -0.368
ENSG00000140577 E045 387.0155241 0.0004846570 5.712009e-01 6.975453e-01 15 90641935 90642998 1064 + 2.548 2.579 0.103
ENSG00000140577 E046 171.4754671 0.0002175811 6.012528e-04 2.537833e-03 15 90642999 90643358 360 + 2.123 2.262 0.464
ENSG00000140577 E047 67.6565588 0.0004068194 3.908959e-02 8.935524e-02 15 90643359 90643416 58 + 1.724 1.858 0.454
ENSG00000140577 E048 60.3561780 0.0005248043 4.370644e-02 9.784870e-02 15 90643417 90643461 45 + 1.676 1.814 0.466
ENSG00000140577 E049 57.3944326 0.0018527038 2.768257e-01 4.163032e-01 15 90643462 90643498 37 + 1.683 1.780 0.328
ENSG00000140577 E050 549.7803201 0.0020955572 1.847414e-09 2.605428e-08 15 90643499 90644658 1160 + 2.593 2.782 0.629
ENSG00000140577 E051 419.8491937 0.0061257128 2.620165e-19 1.547809e-17 15 90644659 90645376 718 + 2.312 2.714 1.339